A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System

Abstract By using near-infrared fluorescent protein (iRFP)-expressing hematopoietic cells, we established a novel, quantitative, in vivo, noninvasive atherosclerosis imaging system. This murine atherosclerosis imaging approach targets macrophages expressing iRFP in plaques. Low-density lipoprotein r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kaushalya Kulathunga, Michito Hamada, Yukiko Hiraishi, Mao Otake, Mai Thi Nhu Tran, Olivia Cheng, Junko Tanaka, Tomoki Sakasai, Shota Sakaguchi, Yuka Sugiyama, Bernd K. Fleischmann, Satoru Takahashi, Yoshihiro Miwa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	In Vivo Imaging System (IVIS) Near-infrared Fluorescent Protein IVIS Images Plaque Macrophages Atherosclerosis Induction Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d61aeb86c6e4b90a0b29fc94a27c589
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d61aeb86c6e4b90a0b29fc94a27c589
record_format	dspace
spelling	oai:doaj.org-article:4d61aeb86c6e4b90a0b29fc94a27c5892021-12-02T11:40:46ZA Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System10.1038/s41598-018-32456-52045-2322https://doaj.org/article/4d61aeb86c6e4b90a0b29fc94a27c5892018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32456-5https://doaj.org/toc/2045-2322Abstract By using near-infrared fluorescent protein (iRFP)-expressing hematopoietic cells, we established a novel, quantitative, in vivo, noninvasive atherosclerosis imaging system. This murine atherosclerosis imaging approach targets macrophages expressing iRFP in plaques. Low-density lipoprotein receptor-deficient (LDLR −/− ) mice transplanted with beta-actin promoter-derived iRFP transgenic (TG) mouse bone marrow (BM) cells (iRFP → LDLR −/− ) were used. Atherosclerosis was induced by a nonfluorescent 1.25% cholesterol diet (HCD). Atherosclerosis was compared among the three differently induced mouse groups. iRFP → LDLR −/− mice fed a normal diet (ND) and LDLR −/− mice transplanted with wild-type (WT) BM cells were used as controls. The in vivo imaging system (IVIS) detected an enhanced iRFP signal in the thoracic aorta of HCD-fed iRFP → LDLR −/− mice, whereas iRFP signals were not observed in the control mice. Time-course imaging showed a gradual increase in the signal area, which was correlated with atherosclerotic plaque progression. Oil red O (ORO) staining of aortas and histological analysis of plaques confirmed that the detected signal was strictly emitted from plaque-positive areas of the aorta. Our new murine atherosclerosis imaging system can noninvasively image atherosclerotic plaques in the aorta and generate longitudinal data, validating the ability of the system to monitor lesion progression.Kaushalya KulathungaMichito HamadaYukiko HiraishiMao OtakeMai Thi Nhu TranOlivia ChengJunko TanakaTomoki SakasaiShota SakaguchiYuka SugiyamaBernd K. FleischmannSatoru TakahashiYoshihiro MiwaNature PortfolioarticleIn Vivo Imaging System (IVIS)Near-infrared Fluorescent ProteinIVIS ImagesPlaque MacrophagesAtherosclerosis InductionMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	In Vivo Imaging System (IVIS) Near-infrared Fluorescent Protein IVIS Images Plaque Macrophages Atherosclerosis Induction Medicine R Science Q
spellingShingle	In Vivo Imaging System (IVIS) Near-infrared Fluorescent Protein IVIS Images Plaque Macrophages Atherosclerosis Induction Medicine R Science Q Kaushalya Kulathunga Michito Hamada Yukiko Hiraishi Mao Otake Mai Thi Nhu Tran Olivia Cheng Junko Tanaka Tomoki Sakasai Shota Sakaguchi Yuka Sugiyama Bernd K. Fleischmann Satoru Takahashi Yoshihiro Miwa A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
description	Abstract By using near-infrared fluorescent protein (iRFP)-expressing hematopoietic cells, we established a novel, quantitative, in vivo, noninvasive atherosclerosis imaging system. This murine atherosclerosis imaging approach targets macrophages expressing iRFP in plaques. Low-density lipoprotein receptor-deficient (LDLR −/− ) mice transplanted with beta-actin promoter-derived iRFP transgenic (TG) mouse bone marrow (BM) cells (iRFP → LDLR −/− ) were used. Atherosclerosis was induced by a nonfluorescent 1.25% cholesterol diet (HCD). Atherosclerosis was compared among the three differently induced mouse groups. iRFP → LDLR −/− mice fed a normal diet (ND) and LDLR −/− mice transplanted with wild-type (WT) BM cells were used as controls. The in vivo imaging system (IVIS) detected an enhanced iRFP signal in the thoracic aorta of HCD-fed iRFP → LDLR −/− mice, whereas iRFP signals were not observed in the control mice. Time-course imaging showed a gradual increase in the signal area, which was correlated with atherosclerotic plaque progression. Oil red O (ORO) staining of aortas and histological analysis of plaques confirmed that the detected signal was strictly emitted from plaque-positive areas of the aorta. Our new murine atherosclerosis imaging system can noninvasively image atherosclerotic plaques in the aorta and generate longitudinal data, validating the ability of the system to monitor lesion progression.
format	article
author	Kaushalya Kulathunga Michito Hamada Yukiko Hiraishi Mao Otake Mai Thi Nhu Tran Olivia Cheng Junko Tanaka Tomoki Sakasai Shota Sakaguchi Yuka Sugiyama Bernd K. Fleischmann Satoru Takahashi Yoshihiro Miwa
author_facet	Kaushalya Kulathunga Michito Hamada Yukiko Hiraishi Mao Otake Mai Thi Nhu Tran Olivia Cheng Junko Tanaka Tomoki Sakasai Shota Sakaguchi Yuka Sugiyama Bernd K. Fleischmann Satoru Takahashi Yoshihiro Miwa
author_sort	Kaushalya Kulathunga
title	A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
title_short	A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
title_full	A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
title_fullStr	A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
title_full_unstemmed	A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System
title_sort	novel irfp-incorporated in vivo murine atherosclerosis imaging system
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4d61aeb86c6e4b90a0b29fc94a27c589
work_keys_str_mv	AT kaushalyakulathunga anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT michitohamada anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT yukikohiraishi anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT maootake anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT maithinhutran anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT oliviacheng anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT junkotanaka anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT tomokisakasai anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT shotasakaguchi anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT yukasugiyama anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT berndkfleischmann anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT satorutakahashi anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT yoshihiromiwa anovelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT kaushalyakulathunga novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT michitohamada novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT yukikohiraishi novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT maootake novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT maithinhutran novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT oliviacheng novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT junkotanaka novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT tomokisakasai novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT shotasakaguchi novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT yukasugiyama novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT berndkfleischmann novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT satorutakahashi novelirfpincorporatedinvivomurineatherosclerosisimagingsystem AT yoshihiromiwa novelirfpincorporatedinvivomurineatherosclerosisimagingsystem
_version_	1718395514314031104

A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System

Ejemplares similares