High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet
Abstract This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanopa...
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Nature Portfolio
2017
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oai:doaj.org-article:4d642a9d2b364ff8a1cd247ec8e4257a2021-12-02T11:40:43ZHigh-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet10.1038/s41598-017-04000-42045-2322https://doaj.org/article/4d642a9d2b364ff8a1cd247ec8e4257a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04000-4https://doaj.org/toc/2045-2322Abstract This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.Shihomi MasudaYuhki YanaseEiji UsukuraSou RyuzakiPangpang WangKoichi OkamotoThasaneeya KubokiSatoru KidoakiKaoru TamadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q |
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Medicine R Science Q Shihomi Masuda Yuhki Yanase Eiji Usukura Sou Ryuzaki Pangpang Wang Koichi Okamoto Thasaneeya Kuboki Satoru Kidoaki Kaoru Tamada High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| description |
Abstract This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics. |
| format |
article |
| author |
Shihomi Masuda Yuhki Yanase Eiji Usukura Sou Ryuzaki Pangpang Wang Koichi Okamoto Thasaneeya Kuboki Satoru Kidoaki Kaoru Tamada |
| author_facet |
Shihomi Masuda Yuhki Yanase Eiji Usukura Sou Ryuzaki Pangpang Wang Koichi Okamoto Thasaneeya Kuboki Satoru Kidoaki Kaoru Tamada |
| author_sort |
Shihomi Masuda |
| title |
High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_short |
High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_full |
High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_fullStr |
High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_full_unstemmed |
High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_sort |
high-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/4d642a9d2b364ff8a1cd247ec8e4257a |
| work_keys_str_mv |
AT shihomimasuda highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT yuhkiyanase highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT eijiusukura highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT souryuzaki highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT pangpangwang highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT koichiokamoto highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT thasaneeyakuboki highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT satorukidoaki highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet AT kaorutamada highresolutionimagingofacellattachednanointerfaceusingagoldnanoparticletwodimensionalsheet |
| _version_ |
1718395579822768128 |