Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus

In this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367–605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive con...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hyeon-Jeong Go, Byung-Joo Park, Hee-Seop Ahn, Dong-Hwi Kim, Da-Yoon Kim, Jae-Hyeong Kim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Yang-Kyu Choi, In-Soo Choi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Hepatitis E virus virus-like particle 239 amino acids viremia fecal viral shedding liver fibrosis Medicine R
Acceso en línea:	https://doaj.org/article/4d64f34af7ef44a28d19c52dc6ea7ff8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d64f34af7ef44a28d19c52dc6ea7ff8
record_format	dspace
spelling	oai:doaj.org-article:4d64f34af7ef44a28d19c52dc6ea7ff82021-11-25T19:10:42ZPigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus10.3390/vaccines91112652076-393Xhttps://doaj.org/article/4d64f34af7ef44a28d19c52dc6ea7ff82021-11-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1265https://doaj.org/toc/2076-393XIn this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367–605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive control, 100 μg VLP-, and 200 μg VLP-vaccinated groups for 10 weeks. The pigs in either of the vaccinated groups were administered the corresponding first and booster doses on weeks 0 and 2. At week 4, the positive control and two vaccinated groups were challenged with 10<sup>6</sup> HEV-3 genomic equivalent copies; viremia and fecal shedding of the virus were identified in pigs in the positive control and 100 μg VLP-vaccinated pigs showed transient viremia and fecal viral shedding. However, no viruses were detected in the serum or fecal samples of the 200 μg VLP-vaccinated pigs. The 100 and 200 μg VLP-vaccinated pigs had significantly higher (<i>p</i> < 0.01) anti-HEV antibodies than the negative control pigs from weeks 6–10 with normal levels of liver enzymes. The 200 μg VLP-vaccinated pigs showed statistically less liver tissue fibrosis (<i>p</i> < 0.05) than that of the positive control pigs. Thus, the novel baculovirus expression system-generated VLP vaccine dose-dependently protects against HEV-3 challenge and may be useful in other animal species, including humans.Hyeon-Jeong GoByung-Joo ParkHee-Seop AhnDong-Hwi KimDa-Yoon KimJae-Hyeong KimJoong-Bok LeeSeung-Yong ParkChang-Seon SongSang-Won LeeYang-Kyu ChoiIn-Soo ChoiMDPI AGarticleHepatitis E virusvirus-like particle239 amino acidsviremiafecal viral sheddingliver fibrosisMedicineRENVaccines, Vol 9, Iss 1265, p 1265 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hepatitis E virus virus-like particle 239 amino acids viremia fecal viral shedding liver fibrosis Medicine R
spellingShingle	Hepatitis E virus virus-like particle 239 amino acids viremia fecal viral shedding liver fibrosis Medicine R Hyeon-Jeong Go Byung-Joo Park Hee-Seop Ahn Dong-Hwi Kim Da-Yoon Kim Jae-Hyeong Kim Joong-Bok Lee Seung-Yong Park Chang-Seon Song Sang-Won Lee Yang-Kyu Choi In-Soo Choi Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus
description	In this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367–605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive control, 100 μg VLP-, and 200 μg VLP-vaccinated groups for 10 weeks. The pigs in either of the vaccinated groups were administered the corresponding first and booster doses on weeks 0 and 2. At week 4, the positive control and two vaccinated groups were challenged with 10<sup>6</sup> HEV-3 genomic equivalent copies; viremia and fecal shedding of the virus were identified in pigs in the positive control and 100 μg VLP-vaccinated pigs showed transient viremia and fecal viral shedding. However, no viruses were detected in the serum or fecal samples of the 200 μg VLP-vaccinated pigs. The 100 and 200 μg VLP-vaccinated pigs had significantly higher (<i>p</i> < 0.01) anti-HEV antibodies than the negative control pigs from weeks 6–10 with normal levels of liver enzymes. The 200 μg VLP-vaccinated pigs showed statistically less liver tissue fibrosis (<i>p</i> < 0.05) than that of the positive control pigs. Thus, the novel baculovirus expression system-generated VLP vaccine dose-dependently protects against HEV-3 challenge and may be useful in other animal species, including humans.
format	article
author	Hyeon-Jeong Go Byung-Joo Park Hee-Seop Ahn Dong-Hwi Kim Da-Yoon Kim Jae-Hyeong Kim Joong-Bok Lee Seung-Yong Park Chang-Seon Song Sang-Won Lee Yang-Kyu Choi In-Soo Choi
author_facet	Hyeon-Jeong Go Byung-Joo Park Hee-Seop Ahn Dong-Hwi Kim Da-Yoon Kim Jae-Hyeong Kim Joong-Bok Lee Seung-Yong Park Chang-Seon Song Sang-Won Lee Yang-Kyu Choi In-Soo Choi
author_sort	Hyeon-Jeong Go
title	Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus
title_short	Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus
title_full	Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus
title_fullStr	Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus
title_full_unstemmed	Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus
title_sort	pigs immunized with the virus-like particle vaccine are protected against the hepatitis e-3 virus
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4d64f34af7ef44a28d19c52dc6ea7ff8
work_keys_str_mv	AT hyeonjeonggo pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT byungjoopark pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT heeseopahn pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT donghwikim pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT dayoonkim pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT jaehyeongkim pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT joongboklee pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT seungyongpark pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT changseonsong pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT sangwonlee pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT yangkyuchoi pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus AT insoochoi pigsimmunizedwiththeviruslikeparticlevaccineareprotectedagainstthehepatitise3virus
_version_	1718410213787172864

Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus

Ejemplares similares