Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds

Yinbo Peng,1 Chenlu Song,1 Chuanfeng Yang,1 Qige Guo,1 Min Yao1,2 1Department of Burns and Plastic Surgery, Shanghai Ninth People’s Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Department of D...

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Autores principales:	Peng Y, Song C, Yang C, Guo Q, Yao M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	silver nanoparticles LMWC-AgNPs MRSA PVP-AgNPs PVP biocompatibility Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4d6dea953cdb44e59bdc4f48c9f9b8f0
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spelling	oai:doaj.org-article:4d6dea953cdb44e59bdc4f48c9f9b8f02021-12-02T02:45:55ZLow molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds1178-2013https://doaj.org/article/4d6dea953cdb44e59bdc4f48c9f9b8f02017-01-01T00:00:00Zhttps://www.dovepress.com/low-molecular-weight-chitosan-coated-silver-nanoparticles-are-effectiv-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yinbo Peng,1 Chenlu Song,1 Chuanfeng Yang,1 Qige Guo,1 Min Yao1,2 1Department of Burns and Plastic Surgery, Shanghai Ninth People’s Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Department of Dermatology, Wellman Center for Photomedicine, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA Abstract: Silver nanoparticles (AgNPs) are being widely applied as topical wound materials; however, accumulated deposition of silver in the liver, spleen, and other main organs may lead to organ damage and dysfunction. We report here that low molecular weight chitosan-coated silver nanoparticles (LMWC-AgNPs) are effective against methicillin-resistant Staphylococcus aureus (MRSA), have better biocompatibility, and have lower body absorption characteristics when compared with polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs) and silver nanoparticles without surface stabilizer (uncoated-AgNPs) in a dorsal MRSA wound infection mouse model. LMWC-AgNPs were synthesized by reducing silver nitrate with low molecular weight chitosan as a stabilizer and reducing agent, while PVP-AgNPs were synthesized using polyvinylpyrrolidone as a stabilizer and ethanol as a reducing agent. AgNPs with different surface stabilizers were identified by UV-visible absorption spectrometry, and particle size was determined by transmission electron microscopy. UV-visible absorption spectra of LMWC-AgNPs, PVP-AgNPs and uncoated-AgNPs were similar and their sizes were in the range of 10–30 nm. In vitro experiments showed that the three types of AgNPs had similar MRSA-killing effects, with obvious effect at 4 µg/mL and 100% effect at 8 µg/mL. Bacteriostatic annulus experiments also showed that all the three types of AgNPs had similar antibacterial inhibitory effect at 10 µg/mL. Cell counting kit-8 assay and Hoechst/propidium iodide (PI) staining showed that LMWC-AgNPs were significantly less toxic to human fibroblasts than PVP-AgNPs and uncoated-AgNPs. Treatment of mice with MRSA wound infection demonstrated that the three types of AgNPs effectively controlled MRSA wound infection and promoted wound healing. After continuous application for 14 days, LMWC-AgNPs-treated mice showed significantly reduced liver dysfunction as demonstrated by the reduced alanine aminotransferase and aspartate aminotransferase levels and liver deposition of silver, in comparison to mice treated with uncoated-AgNPs or PVP-AgNPs. Our results demonstrated that LMWC-AgNPs had good anti-MRSA effects, while harboring a better biocompatibility and lowering the body’s absorption characteristics. Keywords: silver nanoparticles, LMWC-AgNPs, MRSA, PVP-AgNPs, PVP, biocompatibilityPeng YSong CYang CGuo QYao MDove Medical Pressarticlesilver nanoparticlesLMWC-AgNPsMRSAPVP-AgNPsPVPbiocompatibilityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 295-304 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles LMWC-AgNPs MRSA PVP-AgNPs PVP biocompatibility Medicine (General) R5-920
spellingShingle	silver nanoparticles LMWC-AgNPs MRSA PVP-AgNPs PVP biocompatibility Medicine (General) R5-920 Peng Y Song C Yang C Guo Q Yao M Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds
description	Yinbo Peng,1 Chenlu Song,1 Chuanfeng Yang,1 Qige Guo,1 Min Yao1,2 1Department of Burns and Plastic Surgery, Shanghai Ninth People’s Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Department of Dermatology, Wellman Center for Photomedicine, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA Abstract: Silver nanoparticles (AgNPs) are being widely applied as topical wound materials; however, accumulated deposition of silver in the liver, spleen, and other main organs may lead to organ damage and dysfunction. We report here that low molecular weight chitosan-coated silver nanoparticles (LMWC-AgNPs) are effective against methicillin-resistant Staphylococcus aureus (MRSA), have better biocompatibility, and have lower body absorption characteristics when compared with polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs) and silver nanoparticles without surface stabilizer (uncoated-AgNPs) in a dorsal MRSA wound infection mouse model. LMWC-AgNPs were synthesized by reducing silver nitrate with low molecular weight chitosan as a stabilizer and reducing agent, while PVP-AgNPs were synthesized using polyvinylpyrrolidone as a stabilizer and ethanol as a reducing agent. AgNPs with different surface stabilizers were identified by UV-visible absorption spectrometry, and particle size was determined by transmission electron microscopy. UV-visible absorption spectra of LMWC-AgNPs, PVP-AgNPs and uncoated-AgNPs were similar and their sizes were in the range of 10–30 nm. In vitro experiments showed that the three types of AgNPs had similar MRSA-killing effects, with obvious effect at 4 µg/mL and 100% effect at 8 µg/mL. Bacteriostatic annulus experiments also showed that all the three types of AgNPs had similar antibacterial inhibitory effect at 10 µg/mL. Cell counting kit-8 assay and Hoechst/propidium iodide (PI) staining showed that LMWC-AgNPs were significantly less toxic to human fibroblasts than PVP-AgNPs and uncoated-AgNPs. Treatment of mice with MRSA wound infection demonstrated that the three types of AgNPs effectively controlled MRSA wound infection and promoted wound healing. After continuous application for 14 days, LMWC-AgNPs-treated mice showed significantly reduced liver dysfunction as demonstrated by the reduced alanine aminotransferase and aspartate aminotransferase levels and liver deposition of silver, in comparison to mice treated with uncoated-AgNPs or PVP-AgNPs. Our results demonstrated that LMWC-AgNPs had good anti-MRSA effects, while harboring a better biocompatibility and lowering the body’s absorption characteristics. Keywords: silver nanoparticles, LMWC-AgNPs, MRSA, PVP-AgNPs, PVP, biocompatibility
format	article
author	Peng Y Song C Yang C Guo Q Yao M
author_facet	Peng Y Song C Yang C Guo Q Yao M
author_sort	Peng Y
title	Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds
title_short	Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds
title_full	Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds
title_fullStr	Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds
title_full_unstemmed	Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds
title_sort	low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of mrsa-infected wounds
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/4d6dea953cdb44e59bdc4f48c9f9b8f0
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Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds

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