Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.

<h4>Background</h4>The species Yersinia pestis is commonly divided into three classical biovars, Antiqua, Medievalis, and Orientalis, belonging to subspecies pestis pathogenic for human and the (atypical) non-human pathogenic biovar Microtus (alias Pestoides) including several non-pestis...

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Autores principales:	Yanjun Li, Yujun Cui, Yolande Hauck, Mikhail E Platonov, Erhei Dai, Yajun Song, Zhaobiao Guo, Christine Pourcel, Svetlana V Dentovskaya, Andrey P Anisimov, Ruifu Yang, Gilles Vergnaud
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d78feca326f41848b61ddf2722c0708
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spelling	oai:doaj.org-article:4d78feca326f41848b61ddf2722c07082021-11-25T06:21:59ZGenotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.1932-620310.1371/journal.pone.0006000https://doaj.org/article/4d78feca326f41848b61ddf2722c07082009-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19543392/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The species Yersinia pestis is commonly divided into three classical biovars, Antiqua, Medievalis, and Orientalis, belonging to subspecies pestis pathogenic for human and the (atypical) non-human pathogenic biovar Microtus (alias Pestoides) including several non-pestis subspecies. Recent progress in molecular typing methods enables large-scale investigations in the population structure of this species. It is now possible to test hypotheses about its evolution which were proposed decades ago. For instance the three classical biovars of different geographical distributions were suggested to originate from Central Asia. Most investigations so far have focused on the typical pestis subspecies representatives found outside of China, whereas the understanding of the emergence of this human pathogen requires the investigation of strains belonging to subspecies pestis from China and to the Microtus biovar.<h4>Methodology/principal findings</h4>Multi-locus VNTR analysis (MLVA) with 25 loci was performed on a collection of Y. pestis isolates originating from the majority of the known foci worldwide and including typical rhamnose-negative subspecies pestis as well as rhamnose-positive subspecies pestis and biovar Microtus. More than 500 isolates from China, the Former Soviet Union (FSU), Mongolia and a number of other foci around the world were characterized and resolved into 350 different genotypes. The data revealed very close relationships existing between some isolates from widely separated foci as well as very high diversity which can conversely be observed between nearby foci.<h4>Conclusions/significance</h4>The results obtained are in full agreement with the view that the Y. pestis subsp. pestis pathogenic for humans emerged in the Central Asia region between China, Kazakhstan, Russia and Mongolia, only three clones of which spread out of Central Asia. The relationships among the strains in China, Central Asia and the rest of the world based on the MLVA25 assay provide an unprecedented view on the expansion and microevolution of Y. pestis.Yanjun LiYujun CuiYolande HauckMikhail E PlatonovErhei DaiYajun SongZhaobiao GuoChristine PourcelSvetlana V DentovskayaAndrey P AnisimovRuifu YangGilles VergnaudPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 6, p e6000 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanjun Li Yujun Cui Yolande Hauck Mikhail E Platonov Erhei Dai Yajun Song Zhaobiao Guo Christine Pourcel Svetlana V Dentovskaya Andrey P Anisimov Ruifu Yang Gilles Vergnaud Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.
description	<h4>Background</h4>The species Yersinia pestis is commonly divided into three classical biovars, Antiqua, Medievalis, and Orientalis, belonging to subspecies pestis pathogenic for human and the (atypical) non-human pathogenic biovar Microtus (alias Pestoides) including several non-pestis subspecies. Recent progress in molecular typing methods enables large-scale investigations in the population structure of this species. It is now possible to test hypotheses about its evolution which were proposed decades ago. For instance the three classical biovars of different geographical distributions were suggested to originate from Central Asia. Most investigations so far have focused on the typical pestis subspecies representatives found outside of China, whereas the understanding of the emergence of this human pathogen requires the investigation of strains belonging to subspecies pestis from China and to the Microtus biovar.<h4>Methodology/principal findings</h4>Multi-locus VNTR analysis (MLVA) with 25 loci was performed on a collection of Y. pestis isolates originating from the majority of the known foci worldwide and including typical rhamnose-negative subspecies pestis as well as rhamnose-positive subspecies pestis and biovar Microtus. More than 500 isolates from China, the Former Soviet Union (FSU), Mongolia and a number of other foci around the world were characterized and resolved into 350 different genotypes. The data revealed very close relationships existing between some isolates from widely separated foci as well as very high diversity which can conversely be observed between nearby foci.<h4>Conclusions/significance</h4>The results obtained are in full agreement with the view that the Y. pestis subsp. pestis pathogenic for humans emerged in the Central Asia region between China, Kazakhstan, Russia and Mongolia, only three clones of which spread out of Central Asia. The relationships among the strains in China, Central Asia and the rest of the world based on the MLVA25 assay provide an unprecedented view on the expansion and microevolution of Y. pestis.
format	article
author	Yanjun Li Yujun Cui Yolande Hauck Mikhail E Platonov Erhei Dai Yajun Song Zhaobiao Guo Christine Pourcel Svetlana V Dentovskaya Andrey P Anisimov Ruifu Yang Gilles Vergnaud
author_facet	Yanjun Li Yujun Cui Yolande Hauck Mikhail E Platonov Erhei Dai Yajun Song Zhaobiao Guo Christine Pourcel Svetlana V Dentovskaya Andrey P Anisimov Ruifu Yang Gilles Vergnaud
author_sort	Yanjun Li
title	Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.
title_short	Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.
title_full	Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.
title_fullStr	Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.
title_full_unstemmed	Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.
title_sort	genotyping and phylogenetic analysis of yersinia pestis by mlva: insights into the worldwide expansion of central asia plague foci.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/4d78feca326f41848b61ddf2722c0708
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Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci.

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