N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.

It is known that pigs acted as "mixing vessels" for genesis of a new reassortant influenza strain responsible for pandemic H1N1 2009. However, the host factors driving the evolution of a reassorted virus in pigs to 'jump species' resulting in a human outbreak remain unclear. N-gl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nongluk Sriwilaijaroen, Sachiko Kondo, Hirokazu Yagi, Nobuhiro Takemae, Takehiko Saito, Hiroaki Hiramatsu, Koichi Kato, Yasuo Suzuki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d7b0d0eea0d47ba8650098d3af44de0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d7b0d0eea0d47ba8650098d3af44de0
record_format	dspace
spelling	oai:doaj.org-article:4d7b0d0eea0d47ba8650098d3af44de02021-11-18T06:59:02ZN-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.1932-620310.1371/journal.pone.0016302https://doaj.org/article/4d7b0d0eea0d47ba8650098d3af44de02011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21347401/?tool=EBIhttps://doaj.org/toc/1932-6203It is known that pigs acted as "mixing vessels" for genesis of a new reassortant influenza strain responsible for pandemic H1N1 2009. However, the host factors driving the evolution of a reassorted virus in pigs to 'jump species' resulting in a human outbreak remain unclear. N-glycans derived from the porcine respiratory tract were enzymatically released, fluorescent labeled with 2-aminopyridine, separated according to charge, size and hydrophobicity, and structurally identified by a two-dimensional (size and hydrophobicity) HPLC mapping technique and MALDI-TOF mass spectrometry before and after exo-glycosidase digestion. We found a 3-, 5-, and 13-fold increases in NeuAcα2-6, a preferable human influenza receptor, over NeuAcα2-3, an avian influenza receptor, from upper and lower parts of the porcine trachea towards the porcine lung, a major target organ for swine virus replication. The large proportion of NeuAcα2-6 may exert selective pressure for selection of influenza variants with altered receptor preference for this human-type α2-6 receptor, a crucial first step for generating a human pandemic.Nongluk SriwilaijaroenSachiko KondoHirokazu YagiNobuhiro TakemaeTakehiko SaitoHiroaki HiramatsuKoichi KatoYasuo SuzukiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e16302 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nongluk Sriwilaijaroen Sachiko Kondo Hirokazu Yagi Nobuhiro Takemae Takehiko Saito Hiroaki Hiramatsu Koichi Kato Yasuo Suzuki N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.
description	It is known that pigs acted as "mixing vessels" for genesis of a new reassortant influenza strain responsible for pandemic H1N1 2009. However, the host factors driving the evolution of a reassorted virus in pigs to 'jump species' resulting in a human outbreak remain unclear. N-glycans derived from the porcine respiratory tract were enzymatically released, fluorescent labeled with 2-aminopyridine, separated according to charge, size and hydrophobicity, and structurally identified by a two-dimensional (size and hydrophobicity) HPLC mapping technique and MALDI-TOF mass spectrometry before and after exo-glycosidase digestion. We found a 3-, 5-, and 13-fold increases in NeuAcα2-6, a preferable human influenza receptor, over NeuAcα2-3, an avian influenza receptor, from upper and lower parts of the porcine trachea towards the porcine lung, a major target organ for swine virus replication. The large proportion of NeuAcα2-6 may exert selective pressure for selection of influenza variants with altered receptor preference for this human-type α2-6 receptor, a crucial first step for generating a human pandemic.
format	article
author	Nongluk Sriwilaijaroen Sachiko Kondo Hirokazu Yagi Nobuhiro Takemae Takehiko Saito Hiroaki Hiramatsu Koichi Kato Yasuo Suzuki
author_facet	Nongluk Sriwilaijaroen Sachiko Kondo Hirokazu Yagi Nobuhiro Takemae Takehiko Saito Hiroaki Hiramatsu Koichi Kato Yasuo Suzuki
author_sort	Nongluk Sriwilaijaroen
title	N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.
title_short	N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.
title_full	N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.
title_fullStr	N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.
title_full_unstemmed	N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.
title_sort	n-glycans from porcine trachea and lung: predominant neuacα2-6gal could be a selective pressure for influenza variants in favor of human-type receptor.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/4d7b0d0eea0d47ba8650098d3af44de0
work_keys_str_mv	AT nongluksriwilaijaroen nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor AT sachikokondo nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor AT hirokazuyagi nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor AT nobuhirotakemae nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor AT takehikosaito nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor AT hiroakihiramatsu nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor AT koichikato nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor AT yasuosuzuki nglycansfromporcinetracheaandlungpredominantneuaca26galcouldbeaselectivepressureforinfluenzavariantsinfavorofhumantypereceptor
_version_	1718424100882350080

N-glycans from porcine trachea and lung: predominant NeuAcα2-6Gal could be a selective pressure for influenza variants in favor of human-type receptor.

Ejemplares similares