Microinjection Method for Analyzing Zebrafish Early Stage Oocytes

Maternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well understood due to the difficulty of accessing...

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Autores principales:	Manami Kobayashi, Allison Jamieson-Lucy, Mary C. Mullins
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	oocytes balbiani body zebrafish microinjection bucky ball oogenesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4d7c4bdcfcb24c4a8a1d199ca5ff22ea
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spelling	oai:doaj.org-article:4d7c4bdcfcb24c4a8a1d199ca5ff22ea2021-11-08T13:45:41ZMicroinjection Method for Analyzing Zebrafish Early Stage Oocytes2296-634X10.3389/fcell.2021.753642https://doaj.org/article/4d7c4bdcfcb24c4a8a1d199ca5ff22ea2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.753642/fullhttps://doaj.org/toc/2296-634XMaternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well understood due to the difficulty of accessing these oocytes and the lack of analytical methods. Here, we report on a microinjection method for analyzing zebrafish early-stage oocytes and some artifacts to be aware of when performing oocyte injections or analyzing oocytes. Using this method, we successfully injected mRNAs encoding fluorescent-tagged proteins into early-stage oocytes and observed subcellular localization in the live oocytes. This method is expected to advance the functional analysis of genes involved in oogenesis.Manami KobayashiAllison Jamieson-LucyMary C. MullinsFrontiers Media S.A.articleoocytesbalbiani bodyzebrafishmicroinjectionbucky balloogenesisBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oocytes balbiani body zebrafish microinjection bucky ball oogenesis Biology (General) QH301-705.5
spellingShingle	oocytes balbiani body zebrafish microinjection bucky ball oogenesis Biology (General) QH301-705.5 Manami Kobayashi Allison Jamieson-Lucy Mary C. Mullins Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
description	Maternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well understood due to the difficulty of accessing these oocytes and the lack of analytical methods. Here, we report on a microinjection method for analyzing zebrafish early-stage oocytes and some artifacts to be aware of when performing oocyte injections or analyzing oocytes. Using this method, we successfully injected mRNAs encoding fluorescent-tagged proteins into early-stage oocytes and observed subcellular localization in the live oocytes. This method is expected to advance the functional analysis of genes involved in oogenesis.
format	article
author	Manami Kobayashi Allison Jamieson-Lucy Mary C. Mullins
author_facet	Manami Kobayashi Allison Jamieson-Lucy Mary C. Mullins
author_sort	Manami Kobayashi
title	Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
title_short	Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
title_full	Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
title_fullStr	Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
title_full_unstemmed	Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
title_sort	microinjection method for analyzing zebrafish early stage oocytes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4d7c4bdcfcb24c4a8a1d199ca5ff22ea
work_keys_str_mv	AT manamikobayashi microinjectionmethodforanalyzingzebrafishearlystageoocytes AT allisonjamiesonlucy microinjectionmethodforanalyzingzebrafishearlystageoocytes AT marycmullins microinjectionmethodforanalyzingzebrafishearlystageoocytes
_version_	1718442233206669312

Microinjection Method for Analyzing Zebrafish Early Stage Oocytes

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