Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants

Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants

High-throughput genomics of SARS-CoV-2 is essential to characterize virus evolution and to identify adaptations that affect pathogenicity or transmission. While single-nucleotide variations (SNVs) are commonly considered as driving virus adaption, RNA recombination events that delete or insert nucle...

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Auteurs principaux: Elizabeth Jaworski, Rose M Langsjoen, Brooke Mitchell, Barbara Judy, Patrick Newman, Jessica A Plante, Kenneth S Plante, Aaron L Miller, Yiyang Zhou, Daniele Swetnam, Stephanea Sotcheff, Victoria Morris, Nehad Saada, Rafael RG Machado, Allan McConnell, Steven G Widen, Jill Thompson, Jianli Dong, Ping Ren, Rick B Pyles, Thomas G Ksiazek, Vineet D Menachery, Scott C Weaver, Andrew L Routh
Format: article
Langue:EN
Publié: eLife Sciences Publications Ltd 2021
Sujets:
SARS-CoV-2
ClickSeq
Genomics
Nanopore Sequencing
Defective RNAs
Next-Generation Sequencing
Medicine
R
Science
Q
Biology (General)
QH301-705.5
Accès en ligne:https://doaj.org/article/4d7ebe7fd8ba48a3ba01181583b2b50c
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