Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.

Frequent treatment of the growth-restricted (IUGR) ovine fetus with intra-amniotic IGF-1 increases fetal growth. We aimed to determine whether increased growth was maintained with an extended dosing interval and to examine possible mechanisms. Pregnant ewes were allocated to three groups: Control, a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jibran A Wali, Hendrina A de Boo, José G B Derraik, Hui Hui Phua, Mark H Oliver, Frank H Bloomfield, Jane E Harding
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d83986a43544c68860ddb6cf9b67d2d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d83986a43544c68860ddb6cf9b67d2d
record_format	dspace
spelling	oai:doaj.org-article:4d83986a43544c68860ddb6cf9b67d2d2021-11-18T07:17:46ZWeekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.1932-620310.1371/journal.pone.0037899https://doaj.org/article/4d83986a43544c68860ddb6cf9b67d2d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22629469/?tool=EBIhttps://doaj.org/toc/1932-6203Frequent treatment of the growth-restricted (IUGR) ovine fetus with intra-amniotic IGF-1 increases fetal growth. We aimed to determine whether increased growth was maintained with an extended dosing interval and to examine possible mechanisms. Pregnant ewes were allocated to three groups: Control, and two IUGR groups (induced by placental embolization) treated with weekly intra-amniotic injections of either saline (IUGR) or 360 µg IGF-1 (IGF1). IUGR fetuses were hypoxic, hyperuremic, hypoglycemic, and grew more slowly than controls. Placental glucose uptake and SLC2A1 (GLUT2) mRNA levels decreased in IUGR fetuses, but SLC2A3 (GLUT3) and SLC2A4 (GLUT4) levels were unaffected. IGF-1 treatment increased fetal growth rate, did not alter uterine blood flow or placental glucose uptake, and increased placental SLC2A1 and SLC2A4 (but not SLC2A3) mRNA levels compared with saline-treated IUGR animals. Following IGF-1 treatment, placental mRNA levels of isoforms of the system A, y(+), and L amino acid transporters increased 1.3 to 5.0 fold, while the ratio of phosphorylated-mTOR to total mTOR also tended to increase. Weekly intra-amniotic IGF-1 treatment provides a promising avenue for intra-uterine treatment of IUGR babies, and may act via increased fetal substrate supply, up-regulating placental transporters for neutral, cationic, and branched-chain amino acids, possibly via increased activation of the mTOR pathway.Jibran A WaliHendrina A de BooJosé G B DerraikHui Hui PhuaMark H OliverFrank H BloomfieldJane E HardingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37899 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jibran A Wali Hendrina A de Boo José G B Derraik Hui Hui Phua Mark H Oliver Frank H Bloomfield Jane E Harding Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.
description	Frequent treatment of the growth-restricted (IUGR) ovine fetus with intra-amniotic IGF-1 increases fetal growth. We aimed to determine whether increased growth was maintained with an extended dosing interval and to examine possible mechanisms. Pregnant ewes were allocated to three groups: Control, and two IUGR groups (induced by placental embolization) treated with weekly intra-amniotic injections of either saline (IUGR) or 360 µg IGF-1 (IGF1). IUGR fetuses were hypoxic, hyperuremic, hypoglycemic, and grew more slowly than controls. Placental glucose uptake and SLC2A1 (GLUT2) mRNA levels decreased in IUGR fetuses, but SLC2A3 (GLUT3) and SLC2A4 (GLUT4) levels were unaffected. IGF-1 treatment increased fetal growth rate, did not alter uterine blood flow or placental glucose uptake, and increased placental SLC2A1 and SLC2A4 (but not SLC2A3) mRNA levels compared with saline-treated IUGR animals. Following IGF-1 treatment, placental mRNA levels of isoforms of the system A, y(+), and L amino acid transporters increased 1.3 to 5.0 fold, while the ratio of phosphorylated-mTOR to total mTOR also tended to increase. Weekly intra-amniotic IGF-1 treatment provides a promising avenue for intra-uterine treatment of IUGR babies, and may act via increased fetal substrate supply, up-regulating placental transporters for neutral, cationic, and branched-chain amino acids, possibly via increased activation of the mTOR pathway.
format	article
author	Jibran A Wali Hendrina A de Boo José G B Derraik Hui Hui Phua Mark H Oliver Frank H Bloomfield Jane E Harding
author_facet	Jibran A Wali Hendrina A de Boo José G B Derraik Hui Hui Phua Mark H Oliver Frank H Bloomfield Jane E Harding
author_sort	Jibran A Wali
title	Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.
title_short	Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.
title_full	Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.
title_fullStr	Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.
title_full_unstemmed	Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.
title_sort	weekly intra-amniotic igf-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/4d83986a43544c68860ddb6cf9b67d2d
work_keys_str_mv	AT jibranawali weeklyintraamnioticigf1treatmentincreasesgrowthofgrowthrestrictedovinefetusesandupregulatesplacentalaminoacidtransporters AT hendrinaadeboo weeklyintraamnioticigf1treatmentincreasesgrowthofgrowthrestrictedovinefetusesandupregulatesplacentalaminoacidtransporters AT josegbderraik weeklyintraamnioticigf1treatmentincreasesgrowthofgrowthrestrictedovinefetusesandupregulatesplacentalaminoacidtransporters AT huihuiphua weeklyintraamnioticigf1treatmentincreasesgrowthofgrowthrestrictedovinefetusesandupregulatesplacentalaminoacidtransporters AT markholiver weeklyintraamnioticigf1treatmentincreasesgrowthofgrowthrestrictedovinefetusesandupregulatesplacentalaminoacidtransporters AT frankhbloomfield weeklyintraamnioticigf1treatmentincreasesgrowthofgrowthrestrictedovinefetusesandupregulatesplacentalaminoacidtransporters AT janeeharding weeklyintraamnioticigf1treatmentincreasesgrowthofgrowthrestrictedovinefetusesandupregulatesplacentalaminoacidtransporters
_version_	1718423662054342656

Weekly intra-amniotic IGF-1 treatment increases growth of growth-restricted ovine fetuses and up-regulates placental amino acid transporters.

Ejemplares similares