First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)

Colorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients,...

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Autores principales:	Thais B. Rodrigues, Sambit K. Mishra, Krishnakumar Sridharan, Ethann R. Barnes, Andrei Alyokhin, Rich Tuttle, Wimalanathan Kokulapalan, David Garby, Nicholas J. Skizim, Yu-wen Tang, Brian Manley, Lorenzo Aulisa, Ronald D. Flannagan, Carole Cobb, Kenneth E. Narva
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	RNA interference CPB PSMB5 dsPSMB5 Ledprona dsRNA Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/4d88f8aec2394050ac0b2b2d4b7baf3d
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spelling	oai:doaj.org-article:4d88f8aec2394050ac0b2b2d4b7baf3d2021-11-30T14:49:02ZFirst Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)1664-462X10.3389/fpls.2021.728652https://doaj.org/article/4d88f8aec2394050ac0b2b2d4b7baf3d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.728652/fullhttps://doaj.org/toc/1664-462XColorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients, many chemicals are no longer effective in controlling CPB. Ledprona is a sprayable double-stranded RNA biopesticide with a new mode of action that triggers the RNA interference pathway. Laboratory assays with second instar larvae fed Ledprona showed a dose–response where 25×10−6g/L of dsPSMB5 caused 90% mortality after 6days of initial exposure. We also showed that exposure to Ledprona for 6h caused larval mortality and decreased target messenger RNA (mRNA) expression. Decrease in PSMB5 protein levels was observed after 48h of larval exposure to Ledprona. Both PSMB5 mRNA and protein levels did not recover over time. Ledprona efficacy was demonstrated in a whole plant greenhouse trial and performed similarly to spinosad. Ledprona, currently pending registration at EPA, represents a new biopesticide class integrated pest management and insecticide resistance management programs directed against CPB.Thais B. RodriguesSambit K. MishraKrishnakumar SridharanEthann R. BarnesAndrei AlyokhinRich TuttleWimalanathan KokulapalanDavid GarbyNicholas J. SkizimYu-wen TangBrian ManleyLorenzo AulisaRonald D. FlannaganCarole CobbKenneth E. NarvaFrontiers Media S.A.articleRNA interferenceCPBPSMB5dsPSMB5LedpronadsRNAPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA interference CPB PSMB5 dsPSMB5 Ledprona dsRNA Plant culture SB1-1110
spellingShingle	RNA interference CPB PSMB5 dsPSMB5 Ledprona dsRNA Plant culture SB1-1110 Thais B. Rodrigues Sambit K. Mishra Krishnakumar Sridharan Ethann R. Barnes Andrei Alyokhin Rich Tuttle Wimalanathan Kokulapalan David Garby Nicholas J. Skizim Yu-wen Tang Brian Manley Lorenzo Aulisa Ronald D. Flannagan Carole Cobb Kenneth E. Narva First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)
description	Colorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients, many chemicals are no longer effective in controlling CPB. Ledprona is a sprayable double-stranded RNA biopesticide with a new mode of action that triggers the RNA interference pathway. Laboratory assays with second instar larvae fed Ledprona showed a dose–response where 25×10−6g/L of dsPSMB5 caused 90% mortality after 6days of initial exposure. We also showed that exposure to Ledprona for 6h caused larval mortality and decreased target messenger RNA (mRNA) expression. Decrease in PSMB5 protein levels was observed after 48h of larval exposure to Ledprona. Both PSMB5 mRNA and protein levels did not recover over time. Ledprona efficacy was demonstrated in a whole plant greenhouse trial and performed similarly to spinosad. Ledprona, currently pending registration at EPA, represents a new biopesticide class integrated pest management and insecticide resistance management programs directed against CPB.
format	article
author	Thais B. Rodrigues Sambit K. Mishra Krishnakumar Sridharan Ethann R. Barnes Andrei Alyokhin Rich Tuttle Wimalanathan Kokulapalan David Garby Nicholas J. Skizim Yu-wen Tang Brian Manley Lorenzo Aulisa Ronald D. Flannagan Carole Cobb Kenneth E. Narva
author_facet	Thais B. Rodrigues Sambit K. Mishra Krishnakumar Sridharan Ethann R. Barnes Andrei Alyokhin Rich Tuttle Wimalanathan Kokulapalan David Garby Nicholas J. Skizim Yu-wen Tang Brian Manley Lorenzo Aulisa Ronald D. Flannagan Carole Cobb Kenneth E. Narva
author_sort	Thais B. Rodrigues
title	First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)
title_short	First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)
title_full	First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)
title_fullStr	First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)
title_full_unstemmed	First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)
title_sort	first sprayable double-stranded rna-based biopesticide product targets proteasome subunit beta type-5 in colorado potato beetle (leptinotarsa decemlineata)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4d88f8aec2394050ac0b2b2d4b7baf3d
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First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)

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