The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized

ABSTRACT The genome of the bacterium Burkholderia thailandensis encodes three complete LuxI/LuxR-type quorum sensing (QS) systems: BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). The LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3 modulate the expression of target genes...

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Autores principales:	Servane Le Guillouzer, Marie-Christine Groleau, Eric Déziel
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Burkholderia acyl-homoserine lactone gene regulation LuxR/LuxI quorum sensing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4d89b3ec62a04d0dbc23de2eae171b61
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spelling	oai:doaj.org-article:4d89b3ec62a04d0dbc23de2eae171b612021-11-15T15:51:55ZThe Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized10.1128/mBio.01861-172150-7511https://doaj.org/article/4d89b3ec62a04d0dbc23de2eae171b612017-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01861-17https://doaj.org/toc/2150-7511ABSTRACT The genome of the bacterium Burkholderia thailandensis encodes three complete LuxI/LuxR-type quorum sensing (QS) systems: BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). The LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3 modulate the expression of target genes in association with various N-acyl-l-homoserine lactones (AHLs) as signaling molecules produced by the LuxI-type synthases BtaI1, BtaI2, and BtaI3. We have systematically dissected the complex QS circuitry of B. thailandensis strain E264. Direct quantification of N-octanoyl-homoserine lactone (C8-HSL), N-3-hydroxy-decanoyl-homoserine lactone (3OHC10-HSL), and N-3-hydroxy-octanoyl-homoserine lactone (3OHC8-HSL), the primary AHLs produced by this bacterium, was performed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in the wild-type strain and in QS deletion mutants. This was compared to the transcription of btaI1, btaI2, and btaI3 using chromosomal mini-CTX-lux transcriptional reporters. Furthermore, the levels of expression of btaR1, btaR2, and btaR3 were monitored by quantitative reverse transcription-PCR (qRT-PCR). We observed that C8-HSL, 3OHC10-HSL, and 3OHC8-HSL are differentially produced over time during bacterial growth and correlate with the btaI1, btaI2, and btaI3 gene expression profiles, revealing a successive activation of the corresponding QS systems. Moreover, the transcription of the btaR1, btaR2, and btaR3 genes is modulated by cognate and noncognate AHLs, showing that their regulation depends on themselves and on other QS systems. We conclude that the three QS systems in B. thailandensis are interdependent, suggesting that they cooperate dynamically and function in a concerted manner in modulating the expression of QS target genes through a successive regulatory network. IMPORTANCE Quorum sensing (QS) is a widespread bacterial communication system coordinating the expression of specific genes in a cell density-dependent manner and allowing bacteria to synchronize their activities and to function as multicellular communities. QS plays a crucial role in bacterial pathogenicity by regulating the expression of a wide spectrum of virulence/survival factors and is essential to environmental adaptation. The results presented here demonstrate that the multiple QS systems coexisting in the bacterium Burkholderia thailandensis, which is considered the avirulent version of the human pathogen Burkholderia pseudomallei and thus commonly used as an alternative study model, are hierarchically and homeostatically organized. We found these QS systems to be finely integrated into a complex regulatory network, including transcriptional and posttranscriptional interactions, and further incorporating growth stages and temporal expression. These results provide a unique, comprehensive illustration of a sophisticated QS network and will contribute to a better comprehension of the regulatory mechanisms that can be involved in the expression of QS-controlled genes, in particular those associated with the establishment of host-pathogen interactions and acclimatization to the environment.Servane Le GuillouzerMarie-Christine GroleauEric DézielAmerican Society for MicrobiologyarticleBurkholderiaacyl-homoserine lactonegene regulationLuxR/LuxIquorum sensingMicrobiologyQR1-502ENmBio, Vol 8, Iss 6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Burkholderia acyl-homoserine lactone gene regulation LuxR/LuxI quorum sensing Microbiology QR1-502
spellingShingle	Burkholderia acyl-homoserine lactone gene regulation LuxR/LuxI quorum sensing Microbiology QR1-502 Servane Le Guillouzer Marie-Christine Groleau Eric Déziel The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized
description	ABSTRACT The genome of the bacterium Burkholderia thailandensis encodes three complete LuxI/LuxR-type quorum sensing (QS) systems: BtaI1/BtaR1 (QS-1), BtaI2/BtaR2 (QS-2), and BtaI3/BtaR3 (QS-3). The LuxR-type transcriptional regulators BtaR1, BtaR2, and BtaR3 modulate the expression of target genes in association with various N-acyl-l-homoserine lactones (AHLs) as signaling molecules produced by the LuxI-type synthases BtaI1, BtaI2, and BtaI3. We have systematically dissected the complex QS circuitry of B. thailandensis strain E264. Direct quantification of N-octanoyl-homoserine lactone (C8-HSL), N-3-hydroxy-decanoyl-homoserine lactone (3OHC10-HSL), and N-3-hydroxy-octanoyl-homoserine lactone (3OHC8-HSL), the primary AHLs produced by this bacterium, was performed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in the wild-type strain and in QS deletion mutants. This was compared to the transcription of btaI1, btaI2, and btaI3 using chromosomal mini-CTX-lux transcriptional reporters. Furthermore, the levels of expression of btaR1, btaR2, and btaR3 were monitored by quantitative reverse transcription-PCR (qRT-PCR). We observed that C8-HSL, 3OHC10-HSL, and 3OHC8-HSL are differentially produced over time during bacterial growth and correlate with the btaI1, btaI2, and btaI3 gene expression profiles, revealing a successive activation of the corresponding QS systems. Moreover, the transcription of the btaR1, btaR2, and btaR3 genes is modulated by cognate and noncognate AHLs, showing that their regulation depends on themselves and on other QS systems. We conclude that the three QS systems in B. thailandensis are interdependent, suggesting that they cooperate dynamically and function in a concerted manner in modulating the expression of QS target genes through a successive regulatory network. IMPORTANCE Quorum sensing (QS) is a widespread bacterial communication system coordinating the expression of specific genes in a cell density-dependent manner and allowing bacteria to synchronize their activities and to function as multicellular communities. QS plays a crucial role in bacterial pathogenicity by regulating the expression of a wide spectrum of virulence/survival factors and is essential to environmental adaptation. The results presented here demonstrate that the multiple QS systems coexisting in the bacterium Burkholderia thailandensis, which is considered the avirulent version of the human pathogen Burkholderia pseudomallei and thus commonly used as an alternative study model, are hierarchically and homeostatically organized. We found these QS systems to be finely integrated into a complex regulatory network, including transcriptional and posttranscriptional interactions, and further incorporating growth stages and temporal expression. These results provide a unique, comprehensive illustration of a sophisticated QS network and will contribute to a better comprehension of the regulatory mechanisms that can be involved in the expression of QS-controlled genes, in particular those associated with the establishment of host-pathogen interactions and acclimatization to the environment.
format	article
author	Servane Le Guillouzer Marie-Christine Groleau Eric Déziel
author_facet	Servane Le Guillouzer Marie-Christine Groleau Eric Déziel
author_sort	Servane Le Guillouzer
title	The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized
title_short	The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized
title_full	The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized
title_fullStr	The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized
title_full_unstemmed	The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized
title_sort	complex quorum sensing circuitry of <italic toggle="yes">burkholderia thailandensis</italic> is both hierarchically and homeostatically organized
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/4d89b3ec62a04d0dbc23de2eae171b61
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The Complex Quorum Sensing Circuitry of <italic toggle="yes">Burkholderia thailandensis</italic> Is Both Hierarchically and Homeostatically Organized

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