Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.

Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify indiv...

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Autores principales:	João Paulo de Castro Marcondes, Maria Luiza Cotrim Sartor de Oliveira, Alisson M Gontijo, João Lauro Viana de Camargo, Daisy Maria Fávero Salvadori
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4d8aef5b30fe4e60bd9bb3c5e3de912f
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spelling	oai:doaj.org-article:4d8aef5b30fe4e60bd9bb3c5e3de912f2021-11-18T08:36:29ZGenetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.1932-620310.1371/journal.pone.0086162https://doaj.org/article/4d8aef5b30fe4e60bd9bb3c5e3de912f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465937/?tool=EBIhttps://doaj.org/toc/1932-6203Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.João Paulo de Castro MarcondesMaria Luiza Cotrim Sartor de OliveiraAlisson M GontijoJoão Lauro Viana de CamargoDaisy Maria Fávero SalvadoriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86162 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q João Paulo de Castro Marcondes Maria Luiza Cotrim Sartor de Oliveira Alisson M Gontijo João Lauro Viana de Camargo Daisy Maria Fávero Salvadori Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.
description	Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.
format	article
author	João Paulo de Castro Marcondes Maria Luiza Cotrim Sartor de Oliveira Alisson M Gontijo João Lauro Viana de Camargo Daisy Maria Fávero Salvadori
author_facet	João Paulo de Castro Marcondes Maria Luiza Cotrim Sartor de Oliveira Alisson M Gontijo João Lauro Viana de Camargo Daisy Maria Fávero Salvadori
author_sort	João Paulo de Castro Marcondes
title	Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.
title_short	Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.
title_full	Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.
title_fullStr	Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.
title_full_unstemmed	Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.
title_sort	genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4d8aef5b30fe4e60bd9bb3c5e3de912f
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Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma.

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