Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes

BackgroundIdiopathic dilated cardiomyopathy (IDCM) myocardial inflammation may be associated with external triggering factors such as infectious agents. Here, we searched if moderate/severe heart transplantation rejection is related to the presence of myocardial inflammation in IDCM explanted hearts...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jaqueline de Jesus Pereira, Renata Nishiyama Ikegami, Joyce Tiyeko Kawakami, Shérrira Menezes Garavelo, Marcia Martins Reis, Suely Aparecida Pinheiro Palomino, Sandrigo Mangini, Camila Rodrigues Moreno, Samar Freschi de Barros, Aline Rodrigues Souza, Maria de Lourdes Higuchi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cardiomyopathy infectious agents transplantation myocardial rejection Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4d96e0e27a7d40578fa1525e3509c6c5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4d96e0e27a7d40578fa1525e3509c6c5
record_format	dspace
spelling	oai:doaj.org-article:4d96e0e27a7d40578fa1525e3509c6c52021-12-01T13:54:50ZDistinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes2235-298810.3389/fcimb.2021.732276https://doaj.org/article/4d96e0e27a7d40578fa1525e3509c6c52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.732276/fullhttps://doaj.org/toc/2235-2988BackgroundIdiopathic dilated cardiomyopathy (IDCM) myocardial inflammation may be associated with external triggering factors such as infectious agents. Here, we searched if moderate/severe heart transplantation rejection is related to the presence of myocardial inflammation in IDCM explanted hearts, associated with microbial communities.MethodReceptor myocardial samples from 18 explanted hearts were separated into groups according to post-transplant outcome: persistent moderate rejection (PMR; n = 6), moderate rejection (MR; n = 7) that regressed after pulse therapy, and no rejection (NR; n = 5)/light intensity rejection. Inflammation was quantified through immunohistochemistry (IHC), and infectious agents were evaluated by IHC, molecular biology, in situ hybridization technique, and transmission electron microscopy (TEM).ResultsNR presented lower numbers of macrophages, as well as B cells (p = 0.0001), and higher HLA class II expression (p ≤ 0.0001). PMR and MR showed higher levels of Mycoplasma pneumoniae (p = 0.003) and hepatitis B core (p = 0.0009) antigens. NR presented higher levels of parvovirus B19 (PVB19) and human herpes virus 6 (HHV6) and a positive correlation between Borrelia burgdorferi (Bb) and enterovirus genes. Molecular biology demonstrated the presence of M. pneumoniae, Bb, HHV6, and PVB19 genes in all studied groups. TEM revealed structures compatible with the cited microorganisms.ConclusionsThis initial study investigating on infectious agents and inflammation in the IDCM explanted hearts showed that the association between M. pneumoniae and hepatitis B core was associated with a worse outcome after HT, represented by MR and PMR, suggesting that different IDCM microbial communities may be contributing to post-transplant myocardial rejection.Jaqueline de Jesus PereiraJaqueline de Jesus PereiraRenata Nishiyama IkegamiRenata Nishiyama IkegamiJoyce Tiyeko KawakamiJoyce Tiyeko KawakamiShérrira Menezes GaraveloShérrira Menezes GaraveloMarcia Martins ReisMarcia Martins ReisSuely Aparecida Pinheiro PalominoSuely Aparecida Pinheiro PalominoSandrigo ManginiCamila Rodrigues MorenoCamila Rodrigues MorenoSamar Freschi de BarrosAline Rodrigues SouzaAline Rodrigues SouzaMaria de Lourdes HiguchiMaria de Lourdes HiguchiFrontiers Media S.A.articlecardiomyopathyinfectious agentstransplantationmyocardialrejectionMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cardiomyopathy infectious agents transplantation myocardial rejection Microbiology QR1-502
spellingShingle	cardiomyopathy infectious agents transplantation myocardial rejection Microbiology QR1-502 Jaqueline de Jesus Pereira Jaqueline de Jesus Pereira Renata Nishiyama Ikegami Renata Nishiyama Ikegami Joyce Tiyeko Kawakami Joyce Tiyeko Kawakami Shérrira Menezes Garavelo Shérrira Menezes Garavelo Marcia Martins Reis Marcia Martins Reis Suely Aparecida Pinheiro Palomino Suely Aparecida Pinheiro Palomino Sandrigo Mangini Camila Rodrigues Moreno Camila Rodrigues Moreno Samar Freschi de Barros Aline Rodrigues Souza Aline Rodrigues Souza Maria de Lourdes Higuchi Maria de Lourdes Higuchi Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes
description	BackgroundIdiopathic dilated cardiomyopathy (IDCM) myocardial inflammation may be associated with external triggering factors such as infectious agents. Here, we searched if moderate/severe heart transplantation rejection is related to the presence of myocardial inflammation in IDCM explanted hearts, associated with microbial communities.MethodReceptor myocardial samples from 18 explanted hearts were separated into groups according to post-transplant outcome: persistent moderate rejection (PMR; n = 6), moderate rejection (MR; n = 7) that regressed after pulse therapy, and no rejection (NR; n = 5)/light intensity rejection. Inflammation was quantified through immunohistochemistry (IHC), and infectious agents were evaluated by IHC, molecular biology, in situ hybridization technique, and transmission electron microscopy (TEM).ResultsNR presented lower numbers of macrophages, as well as B cells (p = 0.0001), and higher HLA class II expression (p ≤ 0.0001). PMR and MR showed higher levels of Mycoplasma pneumoniae (p = 0.003) and hepatitis B core (p = 0.0009) antigens. NR presented higher levels of parvovirus B19 (PVB19) and human herpes virus 6 (HHV6) and a positive correlation between Borrelia burgdorferi (Bb) and enterovirus genes. Molecular biology demonstrated the presence of M. pneumoniae, Bb, HHV6, and PVB19 genes in all studied groups. TEM revealed structures compatible with the cited microorganisms.ConclusionsThis initial study investigating on infectious agents and inflammation in the IDCM explanted hearts showed that the association between M. pneumoniae and hepatitis B core was associated with a worse outcome after HT, represented by MR and PMR, suggesting that different IDCM microbial communities may be contributing to post-transplant myocardial rejection.
format	article
author	Jaqueline de Jesus Pereira Jaqueline de Jesus Pereira Renata Nishiyama Ikegami Renata Nishiyama Ikegami Joyce Tiyeko Kawakami Joyce Tiyeko Kawakami Shérrira Menezes Garavelo Shérrira Menezes Garavelo Marcia Martins Reis Marcia Martins Reis Suely Aparecida Pinheiro Palomino Suely Aparecida Pinheiro Palomino Sandrigo Mangini Camila Rodrigues Moreno Camila Rodrigues Moreno Samar Freschi de Barros Aline Rodrigues Souza Aline Rodrigues Souza Maria de Lourdes Higuchi Maria de Lourdes Higuchi
author_facet	Jaqueline de Jesus Pereira Jaqueline de Jesus Pereira Renata Nishiyama Ikegami Renata Nishiyama Ikegami Joyce Tiyeko Kawakami Joyce Tiyeko Kawakami Shérrira Menezes Garavelo Shérrira Menezes Garavelo Marcia Martins Reis Marcia Martins Reis Suely Aparecida Pinheiro Palomino Suely Aparecida Pinheiro Palomino Sandrigo Mangini Camila Rodrigues Moreno Camila Rodrigues Moreno Samar Freschi de Barros Aline Rodrigues Souza Aline Rodrigues Souza Maria de Lourdes Higuchi Maria de Lourdes Higuchi
author_sort	Jaqueline de Jesus Pereira
title	Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes
title_short	Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes
title_full	Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes
title_fullStr	Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes
title_full_unstemmed	Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes
title_sort	distinct microbial communities in dilated cardiomyopathy explanted hearts are associated with different myocardial rejection outcomes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4d96e0e27a7d40578fa1525e3509c6c5
work_keys_str_mv	AT jaquelinedejesuspereira distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT jaquelinedejesuspereira distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT renatanishiyamaikegami distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT renatanishiyamaikegami distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT joycetiyekokawakami distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT joycetiyekokawakami distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT sherriramenezesgaravelo distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT sherriramenezesgaravelo distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT marciamartinsreis distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT marciamartinsreis distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT suelyaparecidapinheiropalomino distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT suelyaparecidapinheiropalomino distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT sandrigomangini distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT camilarodriguesmoreno distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT camilarodriguesmoreno distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT samarfreschidebarros distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT alinerodriguessouza distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT alinerodriguessouza distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT mariadelourdeshiguchi distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes AT mariadelourdeshiguchi distinctmicrobialcommunitiesindilatedcardiomyopathyexplantedheartsareassociatedwithdifferentmyocardialrejectionoutcomes
_version_	1718405055769477120

Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes

Ejemplares similares