T cell stiffness is enhanced upon formation of immunological synapse

T cells are activated by target cells via an intimate contact, termed immunological synapse (IS). Cellular mechanical properties, especially stiffness, are essential to regulate cell functions. However, T cell stiffness at a subcellular level at the IS still remains largely elusive. In this work, we...

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Autores principales:	Philipp Jung, Xiangda Zhou, Sandra Iden, Markus Bischoff, Bin Qu
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	T cells stiffness immunological synapse lamellipodia calcium primary CD4+ T cells Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4d9ff2044e054937912db217b796d3fc
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id	oai:doaj.org-article:4d9ff2044e054937912db217b796d3fc
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spelling	oai:doaj.org-article:4d9ff2044e054937912db217b796d3fc2021-11-17T10:46:18ZT cell stiffness is enhanced upon formation of immunological synapse10.7554/eLife.666432050-084Xe66643https://doaj.org/article/4d9ff2044e054937912db217b796d3fc2021-07-01T00:00:00Zhttps://elifesciences.org/articles/66643https://doaj.org/toc/2050-084XT cells are activated by target cells via an intimate contact, termed immunological synapse (IS). Cellular mechanical properties, especially stiffness, are essential to regulate cell functions. However, T cell stiffness at a subcellular level at the IS still remains largely elusive. In this work, we established an atomic force microscopy (AFM)-based elasticity mapping method on whole T cells to obtain an overview of the stiffness with a resolution of ~60 nm. Using primary human CD4+ T cells, we show that when T cells form IS with stimulating antibody-coated surfaces, the lamellipodia are stiffer than the cell body. Upon IS formation, T cell stiffness is enhanced both at the lamellipodia and on the cell body. Chelation of intracellular Ca2+ abolishes IS-induced stiffening at the lamellipodia but has no influence on cell-body-stiffening, suggesting different regulatory mechanisms of IS-induced stiffening at the lamellipodia and the cell body.Philipp JungXiangda ZhouSandra IdenMarkus BischoffBin QueLife Sciences Publications LtdarticleT cellsstiffnessimmunological synapselamellipodiacalciumprimary CD4+ T cellsMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	T cells stiffness immunological synapse lamellipodia calcium primary CD4+ T cells Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	T cells stiffness immunological synapse lamellipodia calcium primary CD4+ T cells Medicine R Science Q Biology (General) QH301-705.5 Philipp Jung Xiangda Zhou Sandra Iden Markus Bischoff Bin Qu T cell stiffness is enhanced upon formation of immunological synapse
description	T cells are activated by target cells via an intimate contact, termed immunological synapse (IS). Cellular mechanical properties, especially stiffness, are essential to regulate cell functions. However, T cell stiffness at a subcellular level at the IS still remains largely elusive. In this work, we established an atomic force microscopy (AFM)-based elasticity mapping method on whole T cells to obtain an overview of the stiffness with a resolution of ~60 nm. Using primary human CD4+ T cells, we show that when T cells form IS with stimulating antibody-coated surfaces, the lamellipodia are stiffer than the cell body. Upon IS formation, T cell stiffness is enhanced both at the lamellipodia and on the cell body. Chelation of intracellular Ca2+ abolishes IS-induced stiffening at the lamellipodia but has no influence on cell-body-stiffening, suggesting different regulatory mechanisms of IS-induced stiffening at the lamellipodia and the cell body.
format	article
author	Philipp Jung Xiangda Zhou Sandra Iden Markus Bischoff Bin Qu
author_facet	Philipp Jung Xiangda Zhou Sandra Iden Markus Bischoff Bin Qu
author_sort	Philipp Jung
title	T cell stiffness is enhanced upon formation of immunological synapse
title_short	T cell stiffness is enhanced upon formation of immunological synapse
title_full	T cell stiffness is enhanced upon formation of immunological synapse
title_fullStr	T cell stiffness is enhanced upon formation of immunological synapse
title_full_unstemmed	T cell stiffness is enhanced upon formation of immunological synapse
title_sort	t cell stiffness is enhanced upon formation of immunological synapse
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/4d9ff2044e054937912db217b796d3fc
work_keys_str_mv	AT philippjung tcellstiffnessisenhanceduponformationofimmunologicalsynapse AT xiangdazhou tcellstiffnessisenhanceduponformationofimmunologicalsynapse AT sandraiden tcellstiffnessisenhanceduponformationofimmunologicalsynapse AT markusbischoff tcellstiffnessisenhanceduponformationofimmunologicalsynapse AT binqu tcellstiffnessisenhanceduponformationofimmunologicalsynapse
_version_	1718425609425649664

T cell stiffness is enhanced upon formation of immunological synapse

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