Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism

Abstract Regeneration failure after spinal cord injury (SCI) results in part from the lack of a pro-regenerative response in injured neurons, but the response to SCI has not been examined specifically in injured sensory neurons. Using RNA sequencing of dorsal root ganglion, we determined that thorac...

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Autores principales:	Eric E. Ewan, Oshri Avraham, Dan Carlin, Tassia Mangetti Gonçalves, Guoyan Zhao, Valeria Cavalli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4da2e4058a074aac9015ac59ef86e45a
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spelling	oai:doaj.org-article:4da2e4058a074aac9015ac59ef86e45a2021-12-02T15:23:03ZAscending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism10.1038/s41598-020-79624-02045-2322https://doaj.org/article/4da2e4058a074aac9015ac59ef86e45a2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79624-0https://doaj.org/toc/2045-2322Abstract Regeneration failure after spinal cord injury (SCI) results in part from the lack of a pro-regenerative response in injured neurons, but the response to SCI has not been examined specifically in injured sensory neurons. Using RNA sequencing of dorsal root ganglion, we determined that thoracic SCI elicits a transcriptional response distinct from sciatic nerve injury (SNI). Both SNI and SCI induced upregulation of ATF3 and Jun, yet this response failed to promote growth in sensory neurons after SCI. RNA sequencing of purified sensory neurons one and three days after injury revealed that unlike SNI, the SCI response is not sustained. Both SCI and SNI elicited the expression of ATF3 target genes, with very little overlap between conditions. Pathway analysis of differentially expressed ATF3 target genes revealed that fatty acid biosynthesis and terpenoid backbone synthesis were downregulated after SCI but not SNI. Pharmacologic inhibition of fatty acid synthase, the enzyme generating palmitic acid, decreased axon growth and regeneration in vitro. These results support the notion that decreased expression of lipid metabolism-related genes after SCI, including fatty acid synthase, may restrict axon regenerative capacity after SCI.Eric E. EwanOshri AvrahamDan CarlinTassia Mangetti GonçalvesGuoyan ZhaoValeria CavalliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eric E. Ewan Oshri Avraham Dan Carlin Tassia Mangetti Gonçalves Guoyan Zhao Valeria Cavalli Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism
description	Abstract Regeneration failure after spinal cord injury (SCI) results in part from the lack of a pro-regenerative response in injured neurons, but the response to SCI has not been examined specifically in injured sensory neurons. Using RNA sequencing of dorsal root ganglion, we determined that thoracic SCI elicits a transcriptional response distinct from sciatic nerve injury (SNI). Both SNI and SCI induced upregulation of ATF3 and Jun, yet this response failed to promote growth in sensory neurons after SCI. RNA sequencing of purified sensory neurons one and three days after injury revealed that unlike SNI, the SCI response is not sustained. Both SCI and SNI elicited the expression of ATF3 target genes, with very little overlap between conditions. Pathway analysis of differentially expressed ATF3 target genes revealed that fatty acid biosynthesis and terpenoid backbone synthesis were downregulated after SCI but not SNI. Pharmacologic inhibition of fatty acid synthase, the enzyme generating palmitic acid, decreased axon growth and regeneration in vitro. These results support the notion that decreased expression of lipid metabolism-related genes after SCI, including fatty acid synthase, may restrict axon regenerative capacity after SCI.
format	article
author	Eric E. Ewan Oshri Avraham Dan Carlin Tassia Mangetti Gonçalves Guoyan Zhao Valeria Cavalli
author_facet	Eric E. Ewan Oshri Avraham Dan Carlin Tassia Mangetti Gonçalves Guoyan Zhao Valeria Cavalli
author_sort	Eric E. Ewan
title	Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism
title_short	Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism
title_full	Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism
title_fullStr	Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism
title_full_unstemmed	Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism
title_sort	ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4da2e4058a074aac9015ac59ef86e45a
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Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism

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