Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles

Abstract The determination of the chemical nature of the organic matter associated with phytoliths remains a challenge. This difficulty mainly stems from amounts of organic carbon (C) that are often well below the detection limit of traditional spectroscopic tools. Conventional solid-state 13C Nucle...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Armand Masion, Anne Alexandre, Fabio Ziarelli, Stéphane Viel, Guaciara M. Santos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4daaa52d1a8445c29e643323b605d5cd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4daaa52d1a8445c29e643323b605d5cd
record_format	dspace
spelling	oai:doaj.org-article:4daaa52d1a8445c29e643323b605d5cd2021-12-02T12:32:39ZDynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles10.1038/s41598-017-03659-z2045-2322https://doaj.org/article/4daaa52d1a8445c29e643323b605d5cd2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03659-zhttps://doaj.org/toc/2045-2322Abstract The determination of the chemical nature of the organic matter associated with phytoliths remains a challenge. This difficulty mainly stems from amounts of organic carbon (C) that are often well below the detection limit of traditional spectroscopic tools. Conventional solid-state 13C Nuclear Magnetic Resonance (NMR) is widely used to examine the nature and structure of organic molecules, but its inherent low sensitivity prohibits the observation of diluted samples. The recent advent of commercial microwave source in the terahertz range triggered a renewed interest in the Dynamic Nuclear Polarization (DNP) technique to improve the signal to noise ratio of solid-state NMR experiments. With this technique, the 13C spectrum of a phytolith sample containing 0.1% w/w C was obtained overnight with sufficient quality to permit a semi-quantitative analysis of the organic matter, showing the presence of peptides and carbohydrates as predominant compounds. Considering the natural abundance of the 13C isotope, this experiment demonstrates that DNP NMR is sufficiently sensitive to observe spin systems present in amounts as low as a few tens of ppm.Armand MasionAnne AlexandreFabio ZiarelliStéphane VielGuaciara M. SantosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Armand Masion Anne Alexandre Fabio Ziarelli Stéphane Viel Guaciara M. Santos Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles
description	Abstract The determination of the chemical nature of the organic matter associated with phytoliths remains a challenge. This difficulty mainly stems from amounts of organic carbon (C) that are often well below the detection limit of traditional spectroscopic tools. Conventional solid-state 13C Nuclear Magnetic Resonance (NMR) is widely used to examine the nature and structure of organic molecules, but its inherent low sensitivity prohibits the observation of diluted samples. The recent advent of commercial microwave source in the terahertz range triggered a renewed interest in the Dynamic Nuclear Polarization (DNP) technique to improve the signal to noise ratio of solid-state NMR experiments. With this technique, the 13C spectrum of a phytolith sample containing 0.1% w/w C was obtained overnight with sufficient quality to permit a semi-quantitative analysis of the organic matter, showing the presence of peptides and carbohydrates as predominant compounds. Considering the natural abundance of the 13C isotope, this experiment demonstrates that DNP NMR is sufficiently sensitive to observe spin systems present in amounts as low as a few tens of ppm.
format	article
author	Armand Masion Anne Alexandre Fabio Ziarelli Stéphane Viel Guaciara M. Santos
author_facet	Armand Masion Anne Alexandre Fabio Ziarelli Stéphane Viel Guaciara M. Santos
author_sort	Armand Masion
title	Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles
title_short	Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles
title_full	Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles
title_fullStr	Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles
title_full_unstemmed	Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles
title_sort	dynamic nuclear polarization nmr as a new tool to investigate the nature of organic compounds occluded in plant silica particles
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4daaa52d1a8445c29e643323b605d5cd
work_keys_str_mv	AT armandmasion dynamicnuclearpolarizationnmrasanewtooltoinvestigatethenatureoforganiccompoundsoccludedinplantsilicaparticles AT annealexandre dynamicnuclearpolarizationnmrasanewtooltoinvestigatethenatureoforganiccompoundsoccludedinplantsilicaparticles AT fabioziarelli dynamicnuclearpolarizationnmrasanewtooltoinvestigatethenatureoforganiccompoundsoccludedinplantsilicaparticles AT stephaneviel dynamicnuclearpolarizationnmrasanewtooltoinvestigatethenatureoforganiccompoundsoccludedinplantsilicaparticles AT guaciaramsantos dynamicnuclearpolarizationnmrasanewtooltoinvestigatethenatureoforganiccompoundsoccludedinplantsilicaparticles
_version_	1718393976574181376

Dynamic Nuclear Polarization NMR as a new tool to investigate the nature of organic compounds occluded in plant silica particles

Ejemplares similares