Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification

Abstract Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo experiments. BALB/c mice were used in...

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Autores principales:	Heekyoung Kang, Hae-Jin Sohn, A-Young Park, A-Jeong Ham, Jeong-Heon Lee, Young-Hwan Oh, Yong-Joon Chwae, Kyongmin Kim, Sun Park, Hongseok Yang, Suk-Yul Jung, Jong-Hyun Kim, Ho-Joon Shin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4dab0f660cf246e39d62d4cfaaedcfde
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spelling	oai:doaj.org-article:4dab0f660cf246e39d62d4cfaaedcfde2021-12-02T10:54:06ZEstablishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification10.1038/s41598-021-83738-42045-2322https://doaj.org/article/4dab0f660cf246e39d62d4cfaaedcfde2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83738-4https://doaj.org/toc/2045-2322Abstract Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo experiments. BALB/c mice were used in this study to establish in vivo AK model. A. castellanii cell suspensions (equal mixtures of trophozoites and cysts) were loaded onto 2-mm contact lens pieces and inserted into mouse eyes that were scratched using an ophthalmic surgical blade under anesthesia and the eyelids of the mice were sutured. The AK signs were grossly observed and PCR was performed using P-FLA primers to amplify the Acanthamoeba 18S-rRNA gene from mouse ocular tissue. The experimental AK mouse model was characterized by typical hazy blurring and melting of the mouse cornea established on day 1 post-inoculation. AK was induced with at least 0.3 × 105 A. castellanii cells (optimal number, 5 × 104), and the infection persisted for two months. The PCR products amplified from the extracted mouse eye DNA confirmed the development of Acanthamoeba-induced keratitis during the infection periods. In conclusion, the present AK mouse model may serve as an important in vivo model for the development of various therapeutic drugs against AK.Heekyoung KangHae-Jin SohnA-Young ParkA-Jeong HamJeong-Heon LeeYoung-Hwan OhYong-Joon ChwaeKyongmin KimSun ParkHongseok YangSuk-Yul JungJong-Hyun KimHo-Joon ShinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heekyoung Kang Hae-Jin Sohn A-Young Park A-Jeong Ham Jeong-Heon Lee Young-Hwan Oh Yong-Joon Chwae Kyongmin Kim Sun Park Hongseok Yang Suk-Yul Jung Jong-Hyun Kim Ho-Joon Shin Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
description	Abstract Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo experiments. BALB/c mice were used in this study to establish in vivo AK model. A. castellanii cell suspensions (equal mixtures of trophozoites and cysts) were loaded onto 2-mm contact lens pieces and inserted into mouse eyes that were scratched using an ophthalmic surgical blade under anesthesia and the eyelids of the mice were sutured. The AK signs were grossly observed and PCR was performed using P-FLA primers to amplify the Acanthamoeba 18S-rRNA gene from mouse ocular tissue. The experimental AK mouse model was characterized by typical hazy blurring and melting of the mouse cornea established on day 1 post-inoculation. AK was induced with at least 0.3 × 105 A. castellanii cells (optimal number, 5 × 104), and the infection persisted for two months. The PCR products amplified from the extracted mouse eye DNA confirmed the development of Acanthamoeba-induced keratitis during the infection periods. In conclusion, the present AK mouse model may serve as an important in vivo model for the development of various therapeutic drugs against AK.
format	article
author	Heekyoung Kang Hae-Jin Sohn A-Young Park A-Jeong Ham Jeong-Heon Lee Young-Hwan Oh Yong-Joon Chwae Kyongmin Kim Sun Park Hongseok Yang Suk-Yul Jung Jong-Hyun Kim Ho-Joon Shin
author_facet	Heekyoung Kang Hae-Jin Sohn A-Young Park A-Jeong Ham Jeong-Heon Lee Young-Hwan Oh Yong-Joon Chwae Kyongmin Kim Sun Park Hongseok Yang Suk-Yul Jung Jong-Hyun Kim Ho-Joon Shin
author_sort	Heekyoung Kang
title	Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
title_short	Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
title_full	Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
title_fullStr	Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
title_full_unstemmed	Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification
title_sort	establishment of an acanthamoeba keratitis mouse model confirmed by amoebic dna amplification
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4dab0f660cf246e39d62d4cfaaedcfde
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Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification

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