Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering

Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering

Rajendiran Rajesh, Y Dominic Ravichandran Organic Chemistry Division, School of Advanced Sciences, VIT University, Vellore, India Abstract: In recent times, tricomponent scaffolds prepared from naturally occurring polysaccharides, hydroxyapatite, and reinforcing materials have been gaining increas...

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Autores principales: Rajesh R, Dominic Ravichandran Y
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/4dabb7000b5f48e5a23c0b9de65efa19
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spelling oai:doaj.org-article:4dabb7000b5f48e5a23c0b9de65efa192021-12-02T00:45:56ZDevelopment of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering1178-2013https://doaj.org/article/4dabb7000b5f48e5a23c0b9de65efa192015-10-01T00:00:00Zhttps://www.dovepress.com/development-of-a-new-carbon-nanotubendashalginatendashhydroxyapatite-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rajendiran Rajesh, Y Dominic Ravichandran Organic Chemistry Division, School of Advanced Sciences, VIT University, Vellore, India Abstract: In recent times, tricomponent scaffolds prepared from naturally occurring polysaccharides, hydroxyapatite, and reinforcing materials have been gaining increased attention in the field of bone tissue engineering. In the current work, a tricomponent scaffold with an oxidized multiwalled carbon nanotube (fMWCNT)–alginate–hydroxyapatite with the required porosity was prepared for the first time by a freeze-drying method and characterized using analytical techniques. The hydroxyapatite for the scaffold was isolated from chicken bones by thermal calcination at 800°C. The Fourier transform infrared spectra and X-ray diffraction data confirmed ionic interactions and formation of the fMWCNT–alginate–hydroxyapatite scaffold. Interconnected porosity with a pore size of 130–170 µm was evident from field emission scanning electron microscopy. The total porosity calculated using the liquid displacement method was found to be 93.85%. In vitro biocompatibility and cell proliferation on the scaffold was checked using an MG-63 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell attachment by Hoechst stain assay. In vitro studies showed better cell proliferation, cell differentiation, and cell attachment on the prepared scaffold. These results indicate that this scaffold could be a promising candidate for bone tissue engineering. Keywords: chicken bone, hydroxyapatite, alginate, tissue engineeringRajesh RDominic Ravichandran YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Supplement 1 Challenges in biomaterials research, Pp 7-15 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Rajesh R
Dominic Ravichandran Y
Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering
description Rajendiran Rajesh, Y Dominic Ravichandran Organic Chemistry Division, School of Advanced Sciences, VIT University, Vellore, India Abstract: In recent times, tricomponent scaffolds prepared from naturally occurring polysaccharides, hydroxyapatite, and reinforcing materials have been gaining increased attention in the field of bone tissue engineering. In the current work, a tricomponent scaffold with an oxidized multiwalled carbon nanotube (fMWCNT)–alginate–hydroxyapatite with the required porosity was prepared for the first time by a freeze-drying method and characterized using analytical techniques. The hydroxyapatite for the scaffold was isolated from chicken bones by thermal calcination at 800°C. The Fourier transform infrared spectra and X-ray diffraction data confirmed ionic interactions and formation of the fMWCNT–alginate–hydroxyapatite scaffold. Interconnected porosity with a pore size of 130–170 µm was evident from field emission scanning electron microscopy. The total porosity calculated using the liquid displacement method was found to be 93.85%. In vitro biocompatibility and cell proliferation on the scaffold was checked using an MG-63 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell attachment by Hoechst stain assay. In vitro studies showed better cell proliferation, cell differentiation, and cell attachment on the prepared scaffold. These results indicate that this scaffold could be a promising candidate for bone tissue engineering. Keywords: chicken bone, hydroxyapatite, alginate, tissue engineering
format article
author Rajesh R
Dominic Ravichandran Y
author_facet Rajesh R
Dominic Ravichandran Y
author_sort Rajesh R
title Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering
title_short Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering
title_full Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering
title_fullStr Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering
title_full_unstemmed Development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering
title_sort development of a new carbon nanotube–alginate–hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/4dabb7000b5f48e5a23c0b9de65efa19
work_keys_str_mv AT rajeshr developmentofanewcarbonnanotubendashalginatendashhydroxyapatitetricomponentcompositescaffoldforapplicationinbonetissueengineering
AT dominicravichandrany developmentofanewcarbonnanotubendashalginatendashhydroxyapatitetricomponentcompositescaffoldforapplicationinbonetissueengineering
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