Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.

Recently, numerous studies have identified that immature cell populations including stem cells and progenitor cells can be found among "side-population" (SP) cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trop...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tomoka Takao, Kazuo Asanoma, Kiyoko Kato, Kotaro Fukushima, Ryosuke Tsunematsu, Toshio Hirakawa, Sueo Matsumura, Hiroyuki Seki, Satoru Takeda, Norio Wake
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4db254af05de43dbab60454446de29c4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4db254af05de43dbab60454446de29c4
record_format	dspace
spelling	oai:doaj.org-article:4db254af05de43dbab60454446de29c42021-11-18T06:50:36ZIsolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.1932-620310.1371/journal.pone.0021990https://doaj.org/article/4db254af05de43dbab60454446de29c42011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21760941/?tool=EBIhttps://doaj.org/toc/1932-6203Recently, numerous studies have identified that immature cell populations including stem cells and progenitor cells can be found among "side-population" (SP) cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP remained to be reported. In this study, HTR-8/SVneo cells and human primary villous cytotrophoblasts (vCTBs) were stained with Hoechst 33342 and SP and non-SP (NSP) fractions were isolated using a cell sorter. A small population of SP cells was identified in HTR-8/SVneo cells and in vCTBs. SP cells expressed several vCTB-specific markers and failed to express syncytiotrophoblast (STB) or extravillous cytotrophopblast (EVT)-specific differentiation markers. SP cells formed colonies and proliferated on mouse embryonic fibroblast (MEF) feeder cells or in MEF conditioned medium supplemented with heparin/FGF2, and they also showed long-term repopulating property. SP cells could differentiate into both STB and EVT cell lineages and expressed several differentiation markers. Microarray analysis revealed that IL7R and IL1R2 were exclusively expressed in SP cells and not in NSP cells. vCTB cells sorted as positive for both IL7R and IL1R2 failed to express trophoblast differentiation markers and spontaneously differentiated into both STB and EVT in basal medium. These features shown by the SP cells suggested that IL7R and IL1R2 are available as markers to detect the SP cells and that vCTB progenitor cells and trophoblast stem cells were involved in the SP cell population.Tomoka TakaoKazuo AsanomaKiyoko KatoKotaro FukushimaRyosuke TsunematsuToshio HirakawaSueo MatsumuraHiroyuki SekiSatoru TakedaNorio WakePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e21990 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomoka Takao Kazuo Asanoma Kiyoko Kato Kotaro Fukushima Ryosuke Tsunematsu Toshio Hirakawa Sueo Matsumura Hiroyuki Seki Satoru Takeda Norio Wake Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
description	Recently, numerous studies have identified that immature cell populations including stem cells and progenitor cells can be found among "side-population" (SP) cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP remained to be reported. In this study, HTR-8/SVneo cells and human primary villous cytotrophoblasts (vCTBs) were stained with Hoechst 33342 and SP and non-SP (NSP) fractions were isolated using a cell sorter. A small population of SP cells was identified in HTR-8/SVneo cells and in vCTBs. SP cells expressed several vCTB-specific markers and failed to express syncytiotrophoblast (STB) or extravillous cytotrophopblast (EVT)-specific differentiation markers. SP cells formed colonies and proliferated on mouse embryonic fibroblast (MEF) feeder cells or in MEF conditioned medium supplemented with heparin/FGF2, and they also showed long-term repopulating property. SP cells could differentiate into both STB and EVT cell lineages and expressed several differentiation markers. Microarray analysis revealed that IL7R and IL1R2 were exclusively expressed in SP cells and not in NSP cells. vCTB cells sorted as positive for both IL7R and IL1R2 failed to express trophoblast differentiation markers and spontaneously differentiated into both STB and EVT in basal medium. These features shown by the SP cells suggested that IL7R and IL1R2 are available as markers to detect the SP cells and that vCTB progenitor cells and trophoblast stem cells were involved in the SP cell population.
format	article
author	Tomoka Takao Kazuo Asanoma Kiyoko Kato Kotaro Fukushima Ryosuke Tsunematsu Toshio Hirakawa Sueo Matsumura Hiroyuki Seki Satoru Takeda Norio Wake
author_facet	Tomoka Takao Kazuo Asanoma Kiyoko Kato Kotaro Fukushima Ryosuke Tsunematsu Toshio Hirakawa Sueo Matsumura Hiroyuki Seki Satoru Takeda Norio Wake
author_sort	Tomoka Takao
title	Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
title_short	Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
title_full	Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
title_fullStr	Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
title_full_unstemmed	Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
title_sort	isolation and characterization of human trophoblast side-population (sp) cells in primary villous cytotrophoblasts and htr-8/svneo cell line.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/4db254af05de43dbab60454446de29c4
work_keys_str_mv	AT tomokatakao isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT kazuoasanoma isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT kiyokokato isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT kotarofukushima isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT ryosuketsunematsu isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT toshiohirakawa isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT sueomatsumura isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT hiroyukiseki isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT satorutakeda isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline AT noriowake isolationandcharacterizationofhumantrophoblastsidepopulationspcellsinprimaryvillouscytotrophoblastsandhtr8svneocellline
_version_	1718424291151708160

Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.

Ejemplares similares