Targeted nanoparticle-mediated LHPP for melanoma treatment

Qianqian Zhang,1,2,* Meimei Xiong,2,* Jinlu Liu,1,2 Shuai Wang,2 Ting Du,2 Tianyi Kang,2 Yu Liu,2 Hao Cheng,2 Meijuan Huang,1 Maling Gou21Department of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China; 2State Key Laboratory of Biothera...

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Autores principales:	Zhang Q, Xiong M, Liu J, Wang S, Du T, Kang T, Liu Y, Cheng H, Huang M, Gou M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Melanoma LHPP Nanoparticle Gene therapy Epigenetics Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4db646cab4524bd68a2d1c6af180b190
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spelling	oai:doaj.org-article:4db646cab4524bd68a2d1c6af180b1902021-12-02T05:40:28ZTargeted nanoparticle-mediated LHPP for melanoma treatment1178-2013https://doaj.org/article/4db646cab4524bd68a2d1c6af180b1902019-05-01T00:00:00Zhttps://www.dovepress.com/targeted-nanoparticle-mediated-lhpp-for-melanoma-treatment-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qianqian Zhang,1,2,* Meimei Xiong,2,* Jinlu Liu,1,2 Shuai Wang,2 Ting Du,2 Tianyi Kang,2 Yu Liu,2 Hao Cheng,2 Meijuan Huang,1 Maling Gou21Department of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China; 2State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China*These authors contributed equally to this workBackground: Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) is a novel tumor suppressor. However, whether LHPP is effective to melanoma has not been investigated. Gene therapy provides a new strategy for the treatment of melanoma. Currently, it suffers from the lack of safe and effective gene delivery systems. Methods: A CRGDKGPDC peptide (iRGD) modified hybrid monomethoxy poly(ethylene glycol)-poly(D,L-lactide) nanoparticle (iDPP) was prepared and complexed with a LHPP plasmid, forming an iDPP/LHPP nanocomplex. The iDPP/LHPP nanocomplex was characterized by particle size distribution, zeta potential, morphology, cytotoxicity, and transfection efficiency. The antitumor efficacy of the nanocomplex against melanoma was studied both in vitro and in vivo. Further, the potential epigenetic changes in melanoma induced by iDPP/LHPP nanocomplex were evaluated.Results: The iDPP/LHPP nanocomplex showed high transfection efficiency and low toxicity. Moreover, the nanocomplex displayed a neutral charge that can meet the requirement of intravenous injection for targeted gene therapy. In vitro and in vivo experiments indicated that the iDPP/LHPP nanocomplex significantly inhibited the melanoma growth without causing notable adverse effects. We also found that LHPP played an important role in epigenetics. It regulated the expression of genes related to the proliferation and apoptosis chiefly at the level of transcription.Conclusion: This work demonstrates that the iDPP nanoparticle-delivered LHPP gene has a potential application in melanoma therapy through regulation of the genes associated with epigenetics.Keywords: melanoma, LHPP, nanoparticle, gene therapy, epigeneticsZhang QXiong MLiu JWang SDu TKang TLiu YCheng HHuang MGou MDove Medical PressarticleMelanomaLHPPNanoparticleGene therapyEpigeneticsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3455-3468 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Melanoma LHPP Nanoparticle Gene therapy Epigenetics Medicine (General) R5-920
spellingShingle	Melanoma LHPP Nanoparticle Gene therapy Epigenetics Medicine (General) R5-920 Zhang Q Xiong M Liu J Wang S Du T Kang T Liu Y Cheng H Huang M Gou M Targeted nanoparticle-mediated LHPP for melanoma treatment
description	Qianqian Zhang,1,2,* Meimei Xiong,2,* Jinlu Liu,1,2 Shuai Wang,2 Ting Du,2 Tianyi Kang,2 Yu Liu,2 Hao Cheng,2 Meijuan Huang,1 Maling Gou21Department of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China; 2State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China*These authors contributed equally to this workBackground: Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) is a novel tumor suppressor. However, whether LHPP is effective to melanoma has not been investigated. Gene therapy provides a new strategy for the treatment of melanoma. Currently, it suffers from the lack of safe and effective gene delivery systems. Methods: A CRGDKGPDC peptide (iRGD) modified hybrid monomethoxy poly(ethylene glycol)-poly(D,L-lactide) nanoparticle (iDPP) was prepared and complexed with a LHPP plasmid, forming an iDPP/LHPP nanocomplex. The iDPP/LHPP nanocomplex was characterized by particle size distribution, zeta potential, morphology, cytotoxicity, and transfection efficiency. The antitumor efficacy of the nanocomplex against melanoma was studied both in vitro and in vivo. Further, the potential epigenetic changes in melanoma induced by iDPP/LHPP nanocomplex were evaluated.Results: The iDPP/LHPP nanocomplex showed high transfection efficiency and low toxicity. Moreover, the nanocomplex displayed a neutral charge that can meet the requirement of intravenous injection for targeted gene therapy. In vitro and in vivo experiments indicated that the iDPP/LHPP nanocomplex significantly inhibited the melanoma growth without causing notable adverse effects. We also found that LHPP played an important role in epigenetics. It regulated the expression of genes related to the proliferation and apoptosis chiefly at the level of transcription.Conclusion: This work demonstrates that the iDPP nanoparticle-delivered LHPP gene has a potential application in melanoma therapy through regulation of the genes associated with epigenetics.Keywords: melanoma, LHPP, nanoparticle, gene therapy, epigenetics
format	article
author	Zhang Q Xiong M Liu J Wang S Du T Kang T Liu Y Cheng H Huang M Gou M
author_facet	Zhang Q Xiong M Liu J Wang S Du T Kang T Liu Y Cheng H Huang M Gou M
author_sort	Zhang Q
title	Targeted nanoparticle-mediated LHPP for melanoma treatment
title_short	Targeted nanoparticle-mediated LHPP for melanoma treatment
title_full	Targeted nanoparticle-mediated LHPP for melanoma treatment
title_fullStr	Targeted nanoparticle-mediated LHPP for melanoma treatment
title_full_unstemmed	Targeted nanoparticle-mediated LHPP for melanoma treatment
title_sort	targeted nanoparticle-mediated lhpp for melanoma treatment
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/4db646cab4524bd68a2d1c6af180b190
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Targeted nanoparticle-mediated LHPP for melanoma treatment

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