Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples

Ancylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was...

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Autores principales:	Héctor Gabriel Avila, Marikena Guadalupe Risso, Marta Cabrera, Paula Ruybal, Silvia Analía Repetto, Marcos Javier Butti, Marcos David Trangoni, Graciela Santillán, Verónica Mirtha Pérez, María Victoria Periago
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	loop mediated isothermal amplification Ancylostoma caninum copro-diagnosis ancylostomiasis molecular diagnosis Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/4dc72f8987ad4898937c92fd0424e038
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spelling	oai:doaj.org-article:4dc72f8987ad4898937c92fd0424e0382021-11-12T05:45:18ZDevelopment of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples2297-176910.3389/fvets.2021.770508https://doaj.org/article/4dc72f8987ad4898937c92fd0424e0382021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.770508/fullhttps://doaj.org/toc/2297-1769Ancylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the cox-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from A. caninum adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of A. caninum for epidemiological studies in endemic areas.Héctor Gabriel AvilaHéctor Gabriel AvilaMarikena Guadalupe RissoMarikena Guadalupe RissoMarta CabreraPaula RuybalPaula RuybalSilvia Analía RepettoSilvia Analía RepettoSilvia Analía RepettoMarcos Javier ButtiMarcos David TrangoniGraciela SantillánVerónica Mirtha PérezVerónica Mirtha PérezMaría Victoria PeriagoMaría Victoria PeriagoFrontiers Media S.A.articleloop mediated isothermal amplificationAncylostoma caninumcopro-diagnosisancylostomiasismolecular diagnosisVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	loop mediated isothermal amplification Ancylostoma caninum copro-diagnosis ancylostomiasis molecular diagnosis Veterinary medicine SF600-1100
spellingShingle	loop mediated isothermal amplification Ancylostoma caninum copro-diagnosis ancylostomiasis molecular diagnosis Veterinary medicine SF600-1100 Héctor Gabriel Avila Héctor Gabriel Avila Marikena Guadalupe Risso Marikena Guadalupe Risso Marta Cabrera Paula Ruybal Paula Ruybal Silvia Analía Repetto Silvia Analía Repetto Silvia Analía Repetto Marcos Javier Butti Marcos David Trangoni Graciela Santillán Verónica Mirtha Pérez Verónica Mirtha Pérez María Victoria Periago María Victoria Periago Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
description	Ancylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the cox-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from A. caninum adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of A. caninum for epidemiological studies in endemic areas.
format	article
author	Héctor Gabriel Avila Héctor Gabriel Avila Marikena Guadalupe Risso Marikena Guadalupe Risso Marta Cabrera Paula Ruybal Paula Ruybal Silvia Analía Repetto Silvia Analía Repetto Silvia Analía Repetto Marcos Javier Butti Marcos David Trangoni Graciela Santillán Verónica Mirtha Pérez Verónica Mirtha Pérez María Victoria Periago María Victoria Periago
author_facet	Héctor Gabriel Avila Héctor Gabriel Avila Marikena Guadalupe Risso Marikena Guadalupe Risso Marta Cabrera Paula Ruybal Paula Ruybal Silvia Analía Repetto Silvia Analía Repetto Silvia Analía Repetto Marcos Javier Butti Marcos David Trangoni Graciela Santillán Verónica Mirtha Pérez Verónica Mirtha Pérez María Victoria Periago María Victoria Periago
author_sort	Héctor Gabriel Avila
title	Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
title_short	Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
title_full	Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
title_fullStr	Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
title_full_unstemmed	Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
title_sort	development of a new lamp assay for the detection of ancylostoma caninum dna (copro-lampac) in dog fecal samples
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4dc72f8987ad4898937c92fd0424e038
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Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples

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