Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes

Abstract Clostridioides difficile infection (CDI) is a major healthcare-associated diarrheal disease. Consistent with trends across the United States, C. difficile RT106 was the second-most prevalent molecular type in our surveillance in Arizona from 2015 to 2018. A representative RT106 strain displ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bryan Angelo P. Roxas, Jennifer Lising Roxas, Rachel Claus-Walker, Anusha Harishankar, Asad Mansoor, Farhan Anwar, Shobitha Jillella, Alison Williams, Jason Lindsey, Sean P. Elliott, Kareem W. Shehab, V. K. Viswanathan, Gayatri Vedantam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4dc744b1c5ff47959c42188205aea95f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4dc744b1c5ff47959c42188205aea95f
record_format	dspace
spelling	oai:doaj.org-article:4dc744b1c5ff47959c42188205aea95f2021-12-02T13:34:10ZPhylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes10.1038/s41598-020-79123-22045-2322https://doaj.org/article/4dc744b1c5ff47959c42188205aea95f2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79123-2https://doaj.org/toc/2045-2322Abstract Clostridioides difficile infection (CDI) is a major healthcare-associated diarrheal disease. Consistent with trends across the United States, C. difficile RT106 was the second-most prevalent molecular type in our surveillance in Arizona from 2015 to 2018. A representative RT106 strain displayed robust virulence and 100% lethality in the hamster model of acute CDI. We identified a unique 46 KB genomic island (GI1) in all RT106 strains sequenced to date, including those in public databases. GI1 was not found in its entirety in any other C. difficile clade, or indeed, in any other microbial genome; however, smaller segments were detected in Enterococcus faecium strains. Molecular clock analyses suggested that GI1 was horizontally acquired and sequentially assembled over time. GI1 encodes homologs of VanZ and a SrtB-anchored collagen-binding adhesin, and correspondingly, all tested RT106 strains had increased teicoplanin resistance, and a majority displayed collagen-dependent biofilm formation. Two additional genomic islands (GI2 and GI3) were also present in a subset of RT106 strains. All three islands are predicted to encode mobile genetic elements as well as virulence factors. Emergent phenotypes associated with these genetic islands may have contributed to the relatively rapid expansion of RT106 in US healthcare and community settings.Bryan Angelo P. RoxasJennifer Lising RoxasRachel Claus-WalkerAnusha HarishankarAsad MansoorFarhan AnwarShobitha JillellaAlison WilliamsJason LindseySean P. ElliottKareem W. ShehabV. K. ViswanathanGayatri VedantamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bryan Angelo P. Roxas Jennifer Lising Roxas Rachel Claus-Walker Anusha Harishankar Asad Mansoor Farhan Anwar Shobitha Jillella Alison Williams Jason Lindsey Sean P. Elliott Kareem W. Shehab V. K. Viswanathan Gayatri Vedantam Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes
description	Abstract Clostridioides difficile infection (CDI) is a major healthcare-associated diarrheal disease. Consistent with trends across the United States, C. difficile RT106 was the second-most prevalent molecular type in our surveillance in Arizona from 2015 to 2018. A representative RT106 strain displayed robust virulence and 100% lethality in the hamster model of acute CDI. We identified a unique 46 KB genomic island (GI1) in all RT106 strains sequenced to date, including those in public databases. GI1 was not found in its entirety in any other C. difficile clade, or indeed, in any other microbial genome; however, smaller segments were detected in Enterococcus faecium strains. Molecular clock analyses suggested that GI1 was horizontally acquired and sequentially assembled over time. GI1 encodes homologs of VanZ and a SrtB-anchored collagen-binding adhesin, and correspondingly, all tested RT106 strains had increased teicoplanin resistance, and a majority displayed collagen-dependent biofilm formation. Two additional genomic islands (GI2 and GI3) were also present in a subset of RT106 strains. All three islands are predicted to encode mobile genetic elements as well as virulence factors. Emergent phenotypes associated with these genetic islands may have contributed to the relatively rapid expansion of RT106 in US healthcare and community settings.
format	article
author	Bryan Angelo P. Roxas Jennifer Lising Roxas Rachel Claus-Walker Anusha Harishankar Asad Mansoor Farhan Anwar Shobitha Jillella Alison Williams Jason Lindsey Sean P. Elliott Kareem W. Shehab V. K. Viswanathan Gayatri Vedantam
author_facet	Bryan Angelo P. Roxas Jennifer Lising Roxas Rachel Claus-Walker Anusha Harishankar Asad Mansoor Farhan Anwar Shobitha Jillella Alison Williams Jason Lindsey Sean P. Elliott Kareem W. Shehab V. K. Viswanathan Gayatri Vedantam
author_sort	Bryan Angelo P. Roxas
title	Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes
title_short	Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes
title_full	Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes
title_fullStr	Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes
title_full_unstemmed	Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes
title_sort	phylogenomic analysis of clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4dc744b1c5ff47959c42188205aea95f
work_keys_str_mv	AT bryanangeloproxas phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT jenniferlisingroxas phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT rachelclauswalker phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT anushaharishankar phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT asadmansoor phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT farhananwar phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT shobithajillella phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT alisonwilliams phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT jasonlindsey phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT seanpelliott phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT kareemwshehab phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT vkviswanathan phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes AT gayatrivedantam phylogenomicanalysisofclostridioidesdifficileribotype106strainsrevealsnovelgeneticislandsandemergentphenotypes
_version_	1718392792488607744

Phylogenomic analysis of Clostridioides difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes

Ejemplares similares