Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology

Abstract We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for...

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Autores principales:	Andrew B. Matheson, Lynn Paterson, Amanda J. Wright, Tania Mendonca, Manlio Tassieri, Paul A. Dalgarno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4dc8345e8fbf436388f61e4d296fcf75
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spelling	oai:doaj.org-article:4dc8345e8fbf436388f61e4d296fcf752021-12-02T13:34:47ZOptical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology10.1038/s41598-021-85013-y2045-2322https://doaj.org/article/4dc8345e8fbf436388f61e4d296fcf752021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85013-yhttps://doaj.org/toc/2045-2322Abstract We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for a high precision z-stage, separate calibration sample, nor a priori knowledge of either the bead size or the optical properties of the suspending medium. Instead, we have developed a simple yet effective in situ spatial calibration method using image sharpness and exploiting the fact we image at multiple planes simultaneously. These features make OpTIMuM an ideal system for microrheology measurements, and we corroborate the effectiveness of this novel microrheology tool by measuring the viscosity of water in three dimensions, simultaneously.Andrew B. MathesonLynn PatersonAmanda J. WrightTania MendoncaManlio TassieriPaul A. DalgarnoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrew B. Matheson Lynn Paterson Amanda J. Wright Tania Mendonca Manlio Tassieri Paul A. Dalgarno Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
description	Abstract We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for a high precision z-stage, separate calibration sample, nor a priori knowledge of either the bead size or the optical properties of the suspending medium. Instead, we have developed a simple yet effective in situ spatial calibration method using image sharpness and exploiting the fact we image at multiple planes simultaneously. These features make OpTIMuM an ideal system for microrheology measurements, and we corroborate the effectiveness of this novel microrheology tool by measuring the viscosity of water in three dimensions, simultaneously.
format	article
author	Andrew B. Matheson Lynn Paterson Amanda J. Wright Tania Mendonca Manlio Tassieri Paul A. Dalgarno
author_facet	Andrew B. Matheson Lynn Paterson Amanda J. Wright Tania Mendonca Manlio Tassieri Paul A. Dalgarno
author_sort	Andrew B. Matheson
title	Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
title_short	Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
title_full	Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
title_fullStr	Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
title_full_unstemmed	Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology
title_sort	optical tweezers with integrated multiplane microscopy (optimum): a new tool for 3d microrheology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4dc8345e8fbf436388f61e4d296fcf75
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Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology

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