The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)

Lima Bean (Phaseolus lunatus L.) contains a lectin called lunatin which is an antinutrient with antioxidant, antifungal, and antiproliferative properties. The purpose of this study was to determine the effect of different processing of Lima bean seeds, namely crushed dry seeds, Lima bean flour, and...

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Autores principales:	Ahmad Nafi', Maria Belgis, Aisyah Fridannisa
Formato:	article
Lenguaje:	EN
Publicado:	Food, Agriculture, and Natural Resources (FANRes) - Networks 2021
Materias:	Agriculture S Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/4dcbd3d1ea804316af0f948ffd24e801
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spelling	oai:doaj.org-article:4dcbd3d1ea804316af0f948ffd24e8012021-12-01T01:17:29ZThe Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)2722-406610.46676/ij-fanres.v2i2.30https://doaj.org/article/4dcbd3d1ea804316af0f948ffd24e8012021-08-01T00:00:00Zhttps://journal.fanres.org/index.php/IJFANRES/article/view/30https://doaj.org/toc/2722-4066Lima Bean (Phaseolus lunatus L.) contains a lectin called lunatin which is an antinutrient with antioxidant, antifungal, and antiproliferative properties. The purpose of this study was to determine the effect of different processing of Lima bean seeds, namely crushed dry seeds, Lima bean flour, and Modified Legume Flour (MOLEF). In addition, it delved into the differences resulting from different extraction temperatures (27ºC, 40ºC, and 50ºC) on the lunatin extract obtained. The results show that the total protein in dry seeds, flour and MOLEF Lima beans are 23.18%, 24.20% and 17.12%, respectively. The molecular weight of lunatin detected is 30.25 kDa; The highest antioxidant activity, marked at 83.58% , is obtained by lunatin extract from samples of crushed dried Lima beans, with an extraction temperature of 50ºC, while the lowest activity, 45.97%, is indicated by lunatin extract from MOLEF samples of Lima beans, with extraction temperature of 40ºC. Temperature variations in general do not affect the protein bands detectable. Likewise, the antioxidant activity also does not show antifungal activity against Aspergillus niger.Ahmad Nafi'Maria BelgisAisyah FridannisaFood, Agriculture, and Natural Resources (FANRes) - NetworksarticleAgricultureSNutrition. Foods and food supplyTX341-641ENInternational Journal on Food, Agriculture and Natural Resources, Vol 2, Iss 2, Pp 7-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Agriculture S Nutrition. Foods and food supply TX341-641
spellingShingle	Agriculture S Nutrition. Foods and food supply TX341-641 Ahmad Nafi' Maria Belgis Aisyah Fridannisa The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)
description	Lima Bean (Phaseolus lunatus L.) contains a lectin called lunatin which is an antinutrient with antioxidant, antifungal, and antiproliferative properties. The purpose of this study was to determine the effect of different processing of Lima bean seeds, namely crushed dry seeds, Lima bean flour, and Modified Legume Flour (MOLEF). In addition, it delved into the differences resulting from different extraction temperatures (27ºC, 40ºC, and 50ºC) on the lunatin extract obtained. The results show that the total protein in dry seeds, flour and MOLEF Lima beans are 23.18%, 24.20% and 17.12%, respectively. The molecular weight of lunatin detected is 30.25 kDa; The highest antioxidant activity, marked at 83.58% , is obtained by lunatin extract from samples of crushed dried Lima beans, with an extraction temperature of 50ºC, while the lowest activity, 45.97%, is indicated by lunatin extract from MOLEF samples of Lima beans, with extraction temperature of 40ºC. Temperature variations in general do not affect the protein bands detectable. Likewise, the antioxidant activity also does not show antifungal activity against Aspergillus niger.
format	article
author	Ahmad Nafi' Maria Belgis Aisyah Fridannisa
author_facet	Ahmad Nafi' Maria Belgis Aisyah Fridannisa
author_sort	Ahmad Nafi'
title	The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)
title_short	The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)
title_full	The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)
title_fullStr	The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)
title_full_unstemmed	The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)
title_sort	extraction and partial characterization of lunatin from lima bean (phaseolus lunatus l.)
publisher	Food, Agriculture, and Natural Resources (FANRes) - Networks
publishDate	2021
url	https://doaj.org/article/4dcbd3d1ea804316af0f948ffd24e801
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The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)

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