Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT

The large size of nanoparticles prevents rapid extravasation from blood vessels and diffusion into tumors. Multimodal imaging uses the physical properties of one modality to validate the results of another. We aim to demonstrate the use of a targeted thin layer-protected ultra-small gold nanoparticl...

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Autores principales:	Jing Chen, Van Phuc Nguyen, Sangeeta Jaiswal, Xiaoyu Kang, Miki Lee, Yannis M. Paulus, Thomas D. Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanoparticle multimodal imaging photoacoustic heterobivalent peptide Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/4dd0ec84d7c043dda799f4eea587d48f
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spelling	oai:doaj.org-article:4dd0ec84d7c043dda799f4eea587d48f2021-11-25T18:39:08ZThin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT10.3390/ph141110751424-8247https://doaj.org/article/4dd0ec84d7c043dda799f4eea587d48f2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1075https://doaj.org/toc/1424-8247The large size of nanoparticles prevents rapid extravasation from blood vessels and diffusion into tumors. Multimodal imaging uses the physical properties of one modality to validate the results of another. We aim to demonstrate the use of a targeted thin layer-protected ultra-small gold nanoparticles (Au-NPs) to detect cancer in vivo using multimodal imaging with photoacoustic and computed tomography (CT). The thin layer was produced using a mixed thiol-containing short ligands, including MUA, CVVVT-ol, and HS-(CH2)<sub>11</sub>-PEG<sub>4</sub>-OH. The gold nanoparticle was labeled with a heterobivalent (HB) peptide ligand that targets overexpression of epidermal growth factor receptors (EGFR) and ErbB2, hereafter HB-Au-NPs. A human xenograft model of esophageal cancer was used for imaging. HB-Au-NPs show spherical morphology, a core diameter of 4.47 ± 0.8 nm on transmission electron microscopy, and a hydrodynamic diameter of 6.41 ± 0.73 nm on dynamic light scattering. Uptake of HB-Au-NPs was observed only in cancer cells that overexpressed EGFR and ErbB2 using photoacoustic microscopy. Photoacoustic images of tumors in vivo showed peak HB-Au-NPs uptake at 8 h post-injection with systemic clearance by ~48 h. Whole-body images using CT validated specific tumor uptake of HB-Au-NPs in vivo. HB-Au-NPs showed good stability and biocompatibility with fast clearance and contrast-enhancing capability for both photoacoustic and CT imaging. A targeted thin layer-protected gold nanoprobe represents a new platform for molecular imaging and shows promise for early detection and staging of cancer.Jing ChenVan Phuc NguyenSangeeta JaiswalXiaoyu KangMiki LeeYannis M. PaulusThomas D. WangMDPI AGarticlenanoparticlemultimodal imagingphotoacousticheterobivalent peptideMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1075, p 1075 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticle multimodal imaging photoacoustic heterobivalent peptide Medicine R Pharmacy and materia medica RS1-441
spellingShingle	nanoparticle multimodal imaging photoacoustic heterobivalent peptide Medicine R Pharmacy and materia medica RS1-441 Jing Chen Van Phuc Nguyen Sangeeta Jaiswal Xiaoyu Kang Miki Lee Yannis M. Paulus Thomas D. Wang Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT
description	The large size of nanoparticles prevents rapid extravasation from blood vessels and diffusion into tumors. Multimodal imaging uses the physical properties of one modality to validate the results of another. We aim to demonstrate the use of a targeted thin layer-protected ultra-small gold nanoparticles (Au-NPs) to detect cancer in vivo using multimodal imaging with photoacoustic and computed tomography (CT). The thin layer was produced using a mixed thiol-containing short ligands, including MUA, CVVVT-ol, and HS-(CH2)<sub>11</sub>-PEG<sub>4</sub>-OH. The gold nanoparticle was labeled with a heterobivalent (HB) peptide ligand that targets overexpression of epidermal growth factor receptors (EGFR) and ErbB2, hereafter HB-Au-NPs. A human xenograft model of esophageal cancer was used for imaging. HB-Au-NPs show spherical morphology, a core diameter of 4.47 ± 0.8 nm on transmission electron microscopy, and a hydrodynamic diameter of 6.41 ± 0.73 nm on dynamic light scattering. Uptake of HB-Au-NPs was observed only in cancer cells that overexpressed EGFR and ErbB2 using photoacoustic microscopy. Photoacoustic images of tumors in vivo showed peak HB-Au-NPs uptake at 8 h post-injection with systemic clearance by ~48 h. Whole-body images using CT validated specific tumor uptake of HB-Au-NPs in vivo. HB-Au-NPs showed good stability and biocompatibility with fast clearance and contrast-enhancing capability for both photoacoustic and CT imaging. A targeted thin layer-protected gold nanoprobe represents a new platform for molecular imaging and shows promise for early detection and staging of cancer.
format	article
author	Jing Chen Van Phuc Nguyen Sangeeta Jaiswal Xiaoyu Kang Miki Lee Yannis M. Paulus Thomas D. Wang
author_facet	Jing Chen Van Phuc Nguyen Sangeeta Jaiswal Xiaoyu Kang Miki Lee Yannis M. Paulus Thomas D. Wang
author_sort	Jing Chen
title	Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT
title_short	Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT
title_full	Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT
title_fullStr	Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT
title_full_unstemmed	Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT
title_sort	thin layer-protected gold nanoparticles for targeted multimodal imaging with photoacoustic and ct
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4dd0ec84d7c043dda799f4eea587d48f
work_keys_str_mv	AT jingchen thinlayerprotectedgoldnanoparticlesfortargetedmultimodalimagingwithphotoacousticandct AT vanphucnguyen thinlayerprotectedgoldnanoparticlesfortargetedmultimodalimagingwithphotoacousticandct AT sangeetajaiswal thinlayerprotectedgoldnanoparticlesfortargetedmultimodalimagingwithphotoacousticandct AT xiaoyukang thinlayerprotectedgoldnanoparticlesfortargetedmultimodalimagingwithphotoacousticandct AT mikilee thinlayerprotectedgoldnanoparticlesfortargetedmultimodalimagingwithphotoacousticandct AT yannismpaulus thinlayerprotectedgoldnanoparticlesfortargetedmultimodalimagingwithphotoacousticandct AT thomasdwang thinlayerprotectedgoldnanoparticlesfortargetedmultimodalimagingwithphotoacousticandct
_version_	1718410832592764928

Thin Layer-Protected Gold Nanoparticles for Targeted Multimodal Imaging with Photoacoustic and CT

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