Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix

Dong-Jin Lim,1,* Adinarayana Andukuri,1,* Jeremy B Vines,1,2 Shibli M Rahman,1 Patrick TJ Hwang,1 Jeonga Kim,3 Anath Shalev,4,6 John A Corbett,5 Ho-Wook Jun1,6 1Department of Biomedical Engineering, University of Alabama at Birmingham, 2Nutech Medical Inc, 3Department of Medicine, Division of Endoc...

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Autores principales:	Lim DJ, Andukuri A, Vines JB, Rahman SM, Hwang PTJ, Kim J, Shalev A, Corbett JA, Jun HW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4dd27510727042b6845154e6ddfbe095
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spelling	oai:doaj.org-article:4dd27510727042b6845154e6ddfbe0952021-12-02T01:50:20ZEnhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix1178-2013https://doaj.org/article/4dd27510727042b6845154e6ddfbe0952014-05-01T00:00:00Zhttp://www.dovepress.com/enhanced-min-6-beta-cell-survival-and-function-on-a-nitric-oxide-relea-a16688https://doaj.org/toc/1178-2013 Dong-Jin Lim,1,* Adinarayana Andukuri,1,* Jeremy B Vines,1,2 Shibli M Rahman,1 Patrick TJ Hwang,1 Jeonga Kim,3 Anath Shalev,4,6 John A Corbett,5 Ho-Wook Jun1,6 1Department of Biomedical Engineering, University of Alabama at Birmingham, 2Nutech Medical Inc, 3Department of Medicine, Division of Endocrinology, Diabetes and Metabolism, 4Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, 5Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, 6Comprehensive Diabetes Center, University of Alabama at Birmingham, Birmingham, AL, USA*These authors contributed equally to this workAbstract: Innovative biomaterial strategies are required to improve islet cell retention, viability, and functionality, and thereby obtain clinically successful outcomes from pancreatic islet cell transplantation. To address this need, we have developed a peptide amphiphile-based nanomatrix that incorporates multifunctional bioactive cues and sustained release of nitric oxide. The goal of this study was to evaluate the effect of this peptide amphiphile nanomatrix on the viability and functionality of MIN-6 islet cells. Additionally, this study provides insight into the role of nitric oxide in islet cell biology, given that conventional nitric oxide donors are unable to release nitric oxide in a controlled, sustained manner, leading to ambiguous results. It was hypothesized that controlled nitric oxide release in synergy with multifunctional bioactive cues would promote islet cell viability and functionality. Nitric oxide-releasing peptide amphiphile nanomatrices within the range of 16.25 µmol to 130 µmol were used to analyze MIN-6 cell behavior. Both 32.5 µmol and 65 µmol peptide amphiphiles showed improved MIN-6 functionality in response to glucose over a 7-day time period, and the elevated functionality was correlated with both PDX-1 and insulin gene expression. Our results demonstrate that nitric oxide has a beneficial effect on MIN-6 cells in a concentration-dependent manner.Keywords: nitric oxide, peptide amphiphile, biomimetic, diabetes, beta cellsLim DJAndukuri AVines JBRahman SMHwang PTJKim JShalev ACorbett JAJun HWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Supplement 1, Pp 13-21 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lim DJ Andukuri A Vines JB Rahman SM Hwang PTJ Kim J Shalev A Corbett JA Jun HW Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix
description	Dong-Jin Lim,1,* Adinarayana Andukuri,1,* Jeremy B Vines,1,2 Shibli M Rahman,1 Patrick TJ Hwang,1 Jeonga Kim,3 Anath Shalev,4,6 John A Corbett,5 Ho-Wook Jun1,6 1Department of Biomedical Engineering, University of Alabama at Birmingham, 2Nutech Medical Inc, 3Department of Medicine, Division of Endocrinology, Diabetes and Metabolism, 4Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, 5Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, 6Comprehensive Diabetes Center, University of Alabama at Birmingham, Birmingham, AL, USA*These authors contributed equally to this workAbstract: Innovative biomaterial strategies are required to improve islet cell retention, viability, and functionality, and thereby obtain clinically successful outcomes from pancreatic islet cell transplantation. To address this need, we have developed a peptide amphiphile-based nanomatrix that incorporates multifunctional bioactive cues and sustained release of nitric oxide. The goal of this study was to evaluate the effect of this peptide amphiphile nanomatrix on the viability and functionality of MIN-6 islet cells. Additionally, this study provides insight into the role of nitric oxide in islet cell biology, given that conventional nitric oxide donors are unable to release nitric oxide in a controlled, sustained manner, leading to ambiguous results. It was hypothesized that controlled nitric oxide release in synergy with multifunctional bioactive cues would promote islet cell viability and functionality. Nitric oxide-releasing peptide amphiphile nanomatrices within the range of 16.25 µmol to 130 µmol were used to analyze MIN-6 cell behavior. Both 32.5 µmol and 65 µmol peptide amphiphiles showed improved MIN-6 functionality in response to glucose over a 7-day time period, and the elevated functionality was correlated with both PDX-1 and insulin gene expression. Our results demonstrate that nitric oxide has a beneficial effect on MIN-6 cells in a concentration-dependent manner.Keywords: nitric oxide, peptide amphiphile, biomimetic, diabetes, beta cells
format	article
author	Lim DJ Andukuri A Vines JB Rahman SM Hwang PTJ Kim J Shalev A Corbett JA Jun HW
author_facet	Lim DJ Andukuri A Vines JB Rahman SM Hwang PTJ Kim J Shalev A Corbett JA Jun HW
author_sort	Lim DJ
title	Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix
title_short	Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix
title_full	Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix
title_fullStr	Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix
title_full_unstemmed	Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix
title_sort	enhanced min-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/4dd27510727042b6845154e6ddfbe095
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Enhanced MIN-6 beta cell survival and function on a nitric oxide-releasing peptide amphiphile nanomatrix

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