Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence

Abstract During antral folliculogenesis, developmental competence of prospective oocytes is regulated in large part by the follicular somatic component to prepare the oocyte for the final stage of maturation and subsequent embryo development. The underlying molecular mechanisms are poorly understood...

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Autores principales:	Satoshi Sugimura, Norio Kobayashi, Hiroaki Okae, Tadayuki Yamanouchi, Hideo Matsuda, Takumi Kojima, Akira Yajima, Yutaka Hashiyada, Masahiro Kaneda, Kan Sato, Kei Imai, Kentaro Tanemura, Takahiro Arima, Robert B. Gilchrist
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4dd4b98cf2a14a2297b005f70b5c0ca8
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spelling	oai:doaj.org-article:4dd4b98cf2a14a2297b005f70b5c0ca82021-12-02T15:05:42ZTranscriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence10.1038/s41598-017-07039-52045-2322https://doaj.org/article/4dd4b98cf2a14a2297b005f70b5c0ca82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07039-5https://doaj.org/toc/2045-2322Abstract During antral folliculogenesis, developmental competence of prospective oocytes is regulated in large part by the follicular somatic component to prepare the oocyte for the final stage of maturation and subsequent embryo development. The underlying molecular mechanisms are poorly understood. Oocytes reaching the advanced stage of follicular growth by administration of exogenous follicle-stimulating hormone (FSH) possess higher developmental competence than oocytes in FSH-untreated smaller follicles. In this study, the transcriptomic profile of the cumulus cells from cows receiving FSH administration (FSH-priming) was compared, as a model of high oocyte competence, with that from untreated donor cows (control). Ingenuity Pathway Analysis showed that cumulus cells receiving FSH-priming were rich in down-regulated transcripts associated with cell movement and migration, including the extracellular matrix-related transcripts, probably preventing the disruption of cell-to-cell contacts. Interestingly, the transcriptomic profile of up-regulated genes in the control group was similar to that of granulosa cells from atretic follicles. Interferon regulatory factor 7 was activated as the key upstream regulator of FSH-priming. Thus, acquisition of developmental competence by oocytes can be ensured by the integrity of cumulus cells involved in cell-to-cell communication and cell survival, which may help achieve enhanced oocyte-somatic cell coupling.Satoshi SugimuraNorio KobayashiHiroaki OkaeTadayuki YamanouchiHideo MatsudaTakumi KojimaAkira YajimaYutaka HashiyadaMasahiro KanedaKan SatoKei ImaiKentaro TanemuraTakahiro ArimaRobert B. GilchristNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoshi Sugimura Norio Kobayashi Hiroaki Okae Tadayuki Yamanouchi Hideo Matsuda Takumi Kojima Akira Yajima Yutaka Hashiyada Masahiro Kaneda Kan Sato Kei Imai Kentaro Tanemura Takahiro Arima Robert B. Gilchrist Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
description	Abstract During antral folliculogenesis, developmental competence of prospective oocytes is regulated in large part by the follicular somatic component to prepare the oocyte for the final stage of maturation and subsequent embryo development. The underlying molecular mechanisms are poorly understood. Oocytes reaching the advanced stage of follicular growth by administration of exogenous follicle-stimulating hormone (FSH) possess higher developmental competence than oocytes in FSH-untreated smaller follicles. In this study, the transcriptomic profile of the cumulus cells from cows receiving FSH administration (FSH-priming) was compared, as a model of high oocyte competence, with that from untreated donor cows (control). Ingenuity Pathway Analysis showed that cumulus cells receiving FSH-priming were rich in down-regulated transcripts associated with cell movement and migration, including the extracellular matrix-related transcripts, probably preventing the disruption of cell-to-cell contacts. Interestingly, the transcriptomic profile of up-regulated genes in the control group was similar to that of granulosa cells from atretic follicles. Interferon regulatory factor 7 was activated as the key upstream regulator of FSH-priming. Thus, acquisition of developmental competence by oocytes can be ensured by the integrity of cumulus cells involved in cell-to-cell communication and cell survival, which may help achieve enhanced oocyte-somatic cell coupling.
format	article
author	Satoshi Sugimura Norio Kobayashi Hiroaki Okae Tadayuki Yamanouchi Hideo Matsuda Takumi Kojima Akira Yajima Yutaka Hashiyada Masahiro Kaneda Kan Sato Kei Imai Kentaro Tanemura Takahiro Arima Robert B. Gilchrist
author_facet	Satoshi Sugimura Norio Kobayashi Hiroaki Okae Tadayuki Yamanouchi Hideo Matsuda Takumi Kojima Akira Yajima Yutaka Hashiyada Masahiro Kaneda Kan Sato Kei Imai Kentaro Tanemura Takahiro Arima Robert B. Gilchrist
author_sort	Satoshi Sugimura
title	Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
title_short	Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
title_full	Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
title_fullStr	Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
title_full_unstemmed	Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
title_sort	transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4dd4b98cf2a14a2297b005f70b5c0ca8
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Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence

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