Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping

Lina Fan,1,* Qiang Wu,1,* Maoquan Chu1,21School of Life Science and Technology, 2The Institute for Advanced Materials and Nano Biomedicine Tongji University, Shanghai, People&#39;s Republic of China *These authors contributed equally to this workBackground: Sentinel lymph node (SLN) mapping...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fan L, Wu Q, Chu M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4dd837a74fc14dcfb8e8db876b091297
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4dd837a74fc14dcfb8e8db876b091297
record_format	dspace
spelling	oai:doaj.org-article:4dd837a74fc14dcfb8e8db876b0912972021-12-02T07:45:58ZNear infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping1176-91141178-2013https://doaj.org/article/4dd837a74fc14dcfb8e8db876b0912972012-06-01T00:00:00Zhttp://www.dovepress.com/near-infrared-fluorescent-chlorophyll-nanoscale-liposomes-for-sentinel-a10171https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Lina Fan,1,* Qiang Wu,1,* Maoquan Chu1,21School of Life Science and Technology, 2The Institute for Advanced Materials and Nano Biomedicine Tongji University, Shanghai, People&#39;s Republic of China *These authors contributed equally to this workBackground: Sentinel lymph node (SLN) mapping using in vivo near infrared fluorescence imaging has attracted great attention during the past few years. Here we report on the early use of poorly water-soluble chlorophyll with near infrared fluorescence extracted from the leaf of Chimonanthus salicifolius, for mouse axillary SLN mapping.Methods and results: To improve the water solubility and SLN targeting of the chlorophyll, we encapsulated the chlorophyll in nanoscale liposomes. The liposome-coated chlorophyll nanocomposites obtained were spherical in shape and had an average diameter of 21.7 &plusmn; 6.0 nm. The nanocomposites dispersed well in water, and in aqueous suspension they exhibited brighter near infrared fluorescence than chlorophyll alone. After incubation of the nanocomposites with normal liver cells (QSG-7701) and macrophage cells (Ana-1) for no more than 48 hours, there was no obvious reduction in cell viability. When the nanocomposites were injected intradermally into the paw of a mouse, the axillary SLN was found to be strongly fluorescent and was easily visualized in real time without a requirement for surgery. The intensity of the near infrared fluorescence emitted by the SLN was obviously brighter than that emitted by the SLN of another mouse that had been intradermally injected with chlorophyll alone.Conclusion: Our data show that the liposome-coated chlorophyll nanocomposites could have great potential for clinical SLN mapping due to their lack of toxicity, bright near infrared fluorescence, and small diameter.Keywords: chlorophyll, liposomes, nanocomposites, near infrared fluorescence, sentinel lymph node mappingFan LWu QChu MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3071-3080 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Fan L Wu Q Chu M Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping
description	Lina Fan,1,* Qiang Wu,1,* Maoquan Chu1,21School of Life Science and Technology, 2The Institute for Advanced Materials and Nano Biomedicine Tongji University, Shanghai, People&#39;s Republic of China *These authors contributed equally to this workBackground: Sentinel lymph node (SLN) mapping using in vivo near infrared fluorescence imaging has attracted great attention during the past few years. Here we report on the early use of poorly water-soluble chlorophyll with near infrared fluorescence extracted from the leaf of Chimonanthus salicifolius, for mouse axillary SLN mapping.Methods and results: To improve the water solubility and SLN targeting of the chlorophyll, we encapsulated the chlorophyll in nanoscale liposomes. The liposome-coated chlorophyll nanocomposites obtained were spherical in shape and had an average diameter of 21.7 &plusmn; 6.0 nm. The nanocomposites dispersed well in water, and in aqueous suspension they exhibited brighter near infrared fluorescence than chlorophyll alone. After incubation of the nanocomposites with normal liver cells (QSG-7701) and macrophage cells (Ana-1) for no more than 48 hours, there was no obvious reduction in cell viability. When the nanocomposites were injected intradermally into the paw of a mouse, the axillary SLN was found to be strongly fluorescent and was easily visualized in real time without a requirement for surgery. The intensity of the near infrared fluorescence emitted by the SLN was obviously brighter than that emitted by the SLN of another mouse that had been intradermally injected with chlorophyll alone.Conclusion: Our data show that the liposome-coated chlorophyll nanocomposites could have great potential for clinical SLN mapping due to their lack of toxicity, bright near infrared fluorescence, and small diameter.Keywords: chlorophyll, liposomes, nanocomposites, near infrared fluorescence, sentinel lymph node mapping
format	article
author	Fan L Wu Q Chu M
author_facet	Fan L Wu Q Chu M
author_sort	Fan L
title	Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping
title_short	Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping
title_full	Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping
title_fullStr	Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping
title_full_unstemmed	Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping
title_sort	near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/4dd837a74fc14dcfb8e8db876b091297
work_keys_str_mv	AT fanl nearinfraredfluorescentchlorophyllnanoscaleliposomesforsentinellymphnodemapping AT wuq nearinfraredfluorescentchlorophyllnanoscaleliposomesforsentinellymphnodemapping AT chum nearinfraredfluorescentchlorophyllnanoscaleliposomesforsentinellymphnodemapping
_version_	1718399152527769600

Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping

Ejemplares similares