Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment

The difficulty students have seeing the color associated with microbial growth in a traditional Winogradsky column can be overcome by substituting diatomaceous earth (DE) for sediment. Microbial growth in a DE column is visible from the early stages of ecological succession and the colors produced a...

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Autor principal: Thomas G. Benoit
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2015
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Acceso en línea:https://doaj.org/article/4ddf3eeea0874e5f9c5681b8302b2bd6
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Sumario:The difficulty students have seeing the color associated with microbial growth in a traditional Winogradsky column can be overcome by substituting diatomaceous earth (DE) for sediment. Microbial growth in a DE column is visible from the early stages of ecological succession and the colors produced appear more vibrant. A flat-sided tissue culture flask can be used as a column container to provide a large surface area for observation. The enhanced visual experience provided by a DE column increases student engagement and learning.Editor's Note:The ASM advocates that students must successfully demonstrate the ability to explain and practice safe laboratory techniques. For more information, read the laboratory safety section of the ASM Curriculum Recommendations: Introductory Course in Microbiology and the Guidelines for Biosafety in Teaching Laboratories, available at www.asm.org. The Editors of JMBE recommend that adopters of the protocols included in this article follow a minimum of Biosafety Level 2 practices.