Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment

The difficulty students have seeing the color associated with microbial growth in a traditional Winogradsky column can be overcome by substituting diatomaceous earth (DE) for sediment. Microbial growth in a DE column is visible from the early stages of ecological succession and the colors produced a...

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Autor principal:	Thomas G. Benoit
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4ddf3eeea0874e5f9c5681b8302b2bd6
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spelling	oai:doaj.org-article:4ddf3eeea0874e5f9c5681b8302b2bd62021-11-15T15:04:05ZIncrease the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment10.1128/jmbe.v16i1.8891935-78851935-7877https://doaj.org/article/4ddf3eeea0874e5f9c5681b8302b2bd62015-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v16i1.889https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885The difficulty students have seeing the color associated with microbial growth in a traditional Winogradsky column can be overcome by substituting diatomaceous earth (DE) for sediment. Microbial growth in a DE column is visible from the early stages of ecological succession and the colors produced appear more vibrant. A flat-sided tissue culture flask can be used as a column container to provide a large surface area for observation. The enhanced visual experience provided by a DE column increases student engagement and learning.Editor's Note:The ASM advocates that students must successfully demonstrate the ability to explain and practice safe laboratory techniques. For more information, read the laboratory safety section of the ASM Curriculum Recommendations: Introductory Course in Microbiology and the Guidelines for Biosafety in Teaching Laboratories, available at www.asm.org. The Editors of JMBE recommend that adopters of the protocols included in this article follow a minimum of Biosafety Level 2 practices.Thomas G. BenoitAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 16, Iss 1, Pp 85-86 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Thomas G. Benoit Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment
description	The difficulty students have seeing the color associated with microbial growth in a traditional Winogradsky column can be overcome by substituting diatomaceous earth (DE) for sediment. Microbial growth in a DE column is visible from the early stages of ecological succession and the colors produced appear more vibrant. A flat-sided tissue culture flask can be used as a column container to provide a large surface area for observation. The enhanced visual experience provided by a DE column increases student engagement and learning.Editor's Note:The ASM advocates that students must successfully demonstrate the ability to explain and practice safe laboratory techniques. For more information, read the laboratory safety section of the ASM Curriculum Recommendations: Introductory Course in Microbiology and the Guidelines for Biosafety in Teaching Laboratories, available at www.asm.org. The Editors of JMBE recommend that adopters of the protocols included in this article follow a minimum of Biosafety Level 2 practices.
format	article
author	Thomas G. Benoit
author_facet	Thomas G. Benoit
author_sort	Thomas G. Benoit
title	Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment
title_short	Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment
title_full	Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment
title_fullStr	Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment
title_full_unstemmed	Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment
title_sort	increase the visibility of microbial growth in a winogradsky column by substituting diatomaceous earth for sediment
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/4ddf3eeea0874e5f9c5681b8302b2bd6
work_keys_str_mv	AT thomasgbenoit increasethevisibilityofmicrobialgrowthinawinogradskycolumnbysubstitutingdiatomaceousearthforsediment
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Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment

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