FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS

Mechanical jaundice (MJ) of malignant genesis is a clinical syndrome caused by obturation of bile ducts due to malignant tumor growth. It is characterized by specific pigmentation of skin, mucous membranes and sclerae due to increased accumulation of bilirubin in blood serum and other fluids and tis...

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Autores principales:	O. V. Smirnova, V. V. Tsukanov, N. M. Titova, B. G. Gubanov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	cytokines enzyme immunoassay mechanical jaundice Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4de146fce5594ed084262bbc3cfaa0fc
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spelling	oai:doaj.org-article:4de146fce5594ed084262bbc3cfaa0fc2021-11-18T08:03:47ZFEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS1563-06252313-741X10.15789/1563-0625-2018-1-135-144https://doaj.org/article/4de146fce5594ed084262bbc3cfaa0fc2018-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1441https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XMechanical jaundice (MJ) of malignant genesis is a clinical syndrome caused by obturation of bile ducts due to malignant tumor growth. It is characterized by specific pigmentation of skin, mucous membranes and sclerae due to increased accumulation of bilirubin in blood serum and other fluids and tissues of the organism. Various immune disorders are detected in patients with mechanical jaundice. Immune reactivity in mechanical jaundice is affected by toxic, infectious, or tumor factors. In connection with these findings, the aim of our study was to measure levels of some pro-inflammatory (IL-2, IL-18, TNFα, IFNγ) and antiinflammatory (IL-4 IL-10) cytokines in patients with malignant disease, depending on serum bilirubin levels. The study included 50 patients with MJ by malignant origin. A control group consisted of 125 practically healthy volunteers comparable with the study group by sex and age. Blood serum cytokine contents in patients and controls was determined by enzyme immunoassay using reagent kits produced by ZAO Vector-Best. Statistical evaluation of data was carried out by means of Statistica for Windows 8.0 and Microsoft Excel software. The levels of IL-2, TNFα, IFNγ and IL-18 were increased before surgery in the patients with mechanical jaundice of malignant genesis, while IL-4 and IL-10 values were reduced in comparison with healthy controls. Altered cytokine regulation in the MJ group manifests as increase in proinflammatory and immunostimulatory cytokine levels, along with decrease in anti-inflammatory cytokine amounts. Predominant feature of immune response may be regarded as prevalence of Th1-immune response when taking into account cytokine levels in blood serum of patients with MJ of malignant genesis. The features of cytokine regulation of patients with malignant MJ depend on the bilirubin content in blood serum. These changes were most frequent in patients with bilirubin level exceeding 60 μmol/L. In general, the quantitative indices of cytokines exhibited unidirectional changes in the groups of MJ patients with different bilirubin levels, except of IL-10. This parameter showed almost 6-fold increase in MJ patients with bilirubin level < 60 μmol/L against control group, and approximately 40 times with respect to the remaining 2 groups. This biological effect may occur due to malignant etiology of the MJ.O. V. SmirnovaV. V. TsukanovN. M. TitovaB. G. GubanovSPb RAACIarticlecytokinesenzyme immunoassaymechanical jaundiceImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 1, Pp 135-144 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	cytokines enzyme immunoassay mechanical jaundice Immunologic diseases. Allergy RC581-607
spellingShingle	cytokines enzyme immunoassay mechanical jaundice Immunologic diseases. Allergy RC581-607 O. V. Smirnova V. V. Tsukanov N. M. Titova B. G. Gubanov FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS
description	Mechanical jaundice (MJ) of malignant genesis is a clinical syndrome caused by obturation of bile ducts due to malignant tumor growth. It is characterized by specific pigmentation of skin, mucous membranes and sclerae due to increased accumulation of bilirubin in blood serum and other fluids and tissues of the organism. Various immune disorders are detected in patients with mechanical jaundice. Immune reactivity in mechanical jaundice is affected by toxic, infectious, or tumor factors. In connection with these findings, the aim of our study was to measure levels of some pro-inflammatory (IL-2, IL-18, TNFα, IFNγ) and antiinflammatory (IL-4 IL-10) cytokines in patients with malignant disease, depending on serum bilirubin levels. The study included 50 patients with MJ by malignant origin. A control group consisted of 125 practically healthy volunteers comparable with the study group by sex and age. Blood serum cytokine contents in patients and controls was determined by enzyme immunoassay using reagent kits produced by ZAO Vector-Best. Statistical evaluation of data was carried out by means of Statistica for Windows 8.0 and Microsoft Excel software. The levels of IL-2, TNFα, IFNγ and IL-18 were increased before surgery in the patients with mechanical jaundice of malignant genesis, while IL-4 and IL-10 values were reduced in comparison with healthy controls. Altered cytokine regulation in the MJ group manifests as increase in proinflammatory and immunostimulatory cytokine levels, along with decrease in anti-inflammatory cytokine amounts. Predominant feature of immune response may be regarded as prevalence of Th1-immune response when taking into account cytokine levels in blood serum of patients with MJ of malignant genesis. The features of cytokine regulation of patients with malignant MJ depend on the bilirubin content in blood serum. These changes were most frequent in patients with bilirubin level exceeding 60 μmol/L. In general, the quantitative indices of cytokines exhibited unidirectional changes in the groups of MJ patients with different bilirubin levels, except of IL-10. This parameter showed almost 6-fold increase in MJ patients with bilirubin level < 60 μmol/L against control group, and approximately 40 times with respect to the remaining 2 groups. This biological effect may occur due to malignant etiology of the MJ.
format	article
author	O. V. Smirnova V. V. Tsukanov N. M. Titova B. G. Gubanov
author_facet	O. V. Smirnova V. V. Tsukanov N. M. Titova B. G. Gubanov
author_sort	O. V. Smirnova
title	FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS
title_short	FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS
title_full	FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS
title_fullStr	FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS
title_full_unstemmed	FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS
title_sort	features of cytokine regulation in patients with mechanical jaundice of malignant genesis
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/4de146fce5594ed084262bbc3cfaa0fc
work_keys_str_mv	AT ovsmirnova featuresofcytokineregulationinpatientswithmechanicaljaundiceofmalignantgenesis AT vvtsukanov featuresofcytokineregulationinpatientswithmechanicaljaundiceofmalignantgenesis AT nmtitova featuresofcytokineregulationinpatientswithmechanicaljaundiceofmalignantgenesis AT bggubanov featuresofcytokineregulationinpatientswithmechanicaljaundiceofmalignantgenesis
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FEATURES OF CYTOKINE REGULATION IN PATIENTS WITH MECHANICAL JAUNDICE OF MALIGNANT GENESIS

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