Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice

Tsgabu Yohannes Araya,1 Aman Karim,2 Gebremedhin Solomon Hailu,1 Gomathi Periasamy,2 Getu Kahsay3 1Department of Medicinal Chemistry, School of Pharmacy, Mekelle University, Mekelle, Ethiopia; 2Department of Pharmacognosy, School of Pharmacy, Mekelle University, Mekelle, Ethiopia; 3Department of Pha...

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Autores principales:	Araya TY, Karim A, Hailu GS, Periasamy G, Kahsay G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	diabetes antidiabetic aloe megalacantha baker streptozotocin thin layer chromatography Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/4de236d1044d4ca390454f60369339af
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spelling	oai:doaj.org-article:4de236d1044d4ca390454f60369339af2021-12-02T13:34:18ZAntihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice1178-7007https://doaj.org/article/4de236d1044d4ca390454f60369339af2021-03-01T00:00:00Zhttps://www.dovepress.com/antihyperglycemic-activity-of-tlc-isolates-from-the-leaves-of-aloe-meg-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Tsgabu Yohannes Araya,1 Aman Karim,2 Gebremedhin Solomon Hailu,1 Gomathi Periasamy,2 Getu Kahsay3 1Department of Medicinal Chemistry, School of Pharmacy, Mekelle University, Mekelle, Ethiopia; 2Department of Pharmacognosy, School of Pharmacy, Mekelle University, Mekelle, Ethiopia; 3Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Mekelle University, Mekelle, EthiopiaCorrespondence: Getu KahsayDepartment of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Mekelle University, P.O. Box 1871, Mekelle, EthiopiaFax +251 34 441 6681Email weldugetu@mu.edu.etBackground: Diabetes mellitus (DM) is a group of metabolic disorders that are characterized by hyperglycemia which results from defects in insulin release or its efficient use by the human body. Although significant progress has been made to manage DM and related complications, it remains a major global health problem. To this end, the search for new antidiabetic drugs from traditionally claimed medicinal plants is important. Aloe megalacantha Baker is an endemic plant used traditionally to treat diabetes in Ethiopia. This study aimed to investigate antidiabetic activity of isolates from the leaf of A. megalacantha Baker in streptozotocin-induced diabetic mice.Methods: The exudate of A. megalacantha was collected by cutting the leaves and scraping the yellow sap and then dried at room temperature. The dried exudate was subjected to repeated thin layer chromatographic (TLC) separations using a mixture of solvent system to isolate the major compounds identified on the TLC plate. A single dose of streptozotocin (50 mg/kg) was injected intraperitoneally to overnight fasted mice to induce diabetes. Antidiabetic activity of TLC isolates was assessed by in vivo methods including oral glucose tolerance test, antihyperglycemic and hypoglycemic activity tests.Results: Three major isolates were obtained from the TLC analysis of the exudate of A. megalacantha. Exudate and TLC isolates were found to be non-toxic up to a dose of 2000 mg/kg. The TLC isolates (Ia and Ib) significantly (p< 0.05) reduced blood glucose levels and also increased body weight change of the diabetic mice compared with control groups.Conclusion: The present study demonstrated the ability of the exudate of A. megalacantha and its TLC isolates to significantly decrease blood glucose levels and increase body weights in mice, thus strengthening the claim of its traditional use in DM-related disorders.Keywords: diabetes, antidiabetic, Aloe megalacantha Baker, streptozotocin, thin layer chromatographyAraya TYKarim AHailu GSPeriasamy GKahsay GDove Medical Pressarticlediabetesantidiabeticaloe megalacantha bakerstreptozotocinthin layer chromatographySpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 1153-1166 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetes antidiabetic aloe megalacantha baker streptozotocin thin layer chromatography Specialties of internal medicine RC581-951
spellingShingle	diabetes antidiabetic aloe megalacantha baker streptozotocin thin layer chromatography Specialties of internal medicine RC581-951 Araya TY Karim A Hailu GS Periasamy G Kahsay G Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice
description	Tsgabu Yohannes Araya,1 Aman Karim,2 Gebremedhin Solomon Hailu,1 Gomathi Periasamy,2 Getu Kahsay3 1Department of Medicinal Chemistry, School of Pharmacy, Mekelle University, Mekelle, Ethiopia; 2Department of Pharmacognosy, School of Pharmacy, Mekelle University, Mekelle, Ethiopia; 3Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Mekelle University, Mekelle, EthiopiaCorrespondence: Getu KahsayDepartment of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Mekelle University, P.O. Box 1871, Mekelle, EthiopiaFax +251 34 441 6681Email weldugetu@mu.edu.etBackground: Diabetes mellitus (DM) is a group of metabolic disorders that are characterized by hyperglycemia which results from defects in insulin release or its efficient use by the human body. Although significant progress has been made to manage DM and related complications, it remains a major global health problem. To this end, the search for new antidiabetic drugs from traditionally claimed medicinal plants is important. Aloe megalacantha Baker is an endemic plant used traditionally to treat diabetes in Ethiopia. This study aimed to investigate antidiabetic activity of isolates from the leaf of A. megalacantha Baker in streptozotocin-induced diabetic mice.Methods: The exudate of A. megalacantha was collected by cutting the leaves and scraping the yellow sap and then dried at room temperature. The dried exudate was subjected to repeated thin layer chromatographic (TLC) separations using a mixture of solvent system to isolate the major compounds identified on the TLC plate. A single dose of streptozotocin (50 mg/kg) was injected intraperitoneally to overnight fasted mice to induce diabetes. Antidiabetic activity of TLC isolates was assessed by in vivo methods including oral glucose tolerance test, antihyperglycemic and hypoglycemic activity tests.Results: Three major isolates were obtained from the TLC analysis of the exudate of A. megalacantha. Exudate and TLC isolates were found to be non-toxic up to a dose of 2000 mg/kg. The TLC isolates (Ia and Ib) significantly (p< 0.05) reduced blood glucose levels and also increased body weight change of the diabetic mice compared with control groups.Conclusion: The present study demonstrated the ability of the exudate of A. megalacantha and its TLC isolates to significantly decrease blood glucose levels and increase body weights in mice, thus strengthening the claim of its traditional use in DM-related disorders.Keywords: diabetes, antidiabetic, Aloe megalacantha Baker, streptozotocin, thin layer chromatography
format	article
author	Araya TY Karim A Hailu GS Periasamy G Kahsay G
author_facet	Araya TY Karim A Hailu GS Periasamy G Kahsay G
author_sort	Araya TY
title	Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice
title_short	Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice
title_full	Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice
title_fullStr	Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice
title_full_unstemmed	Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice
title_sort	antihyperglycemic activity of tlc isolates from the leaves of aloe megalacantha baker in streptozotocin-induced diabetic mice
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/4de236d1044d4ca390454f60369339af
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Antihyperglycemic Activity of TLC Isolates from the Leaves of Aloe megalacantha Baker in Streptozotocin-Induced Diabetic Mice

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