131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy

Na Sun,1,* Lingzhou Zhao,1,* Jingyi Zhu,2,* Yujie Li,1 Ningning Song,1 Yan Xing,1 Wenli Qiao,1 He Huang,2 Jinhua Zhao11Department of Nuclear Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, People’s Republic of China; 2State Key Labora...

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Autores principales:	Sun N, Zhao L, Zhu J, Li Y, Song N, Xing Y, Qiao W, Huang H, Zhao J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Polyethylenimine BmK CT gold nanoparticles SPECT/CT imaging radionuclide therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4df2c196df614991b38a8cb69add9d87
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spelling	oai:doaj.org-article:4df2c196df614991b38a8cb69add9d872021-12-02T10:55:30Z131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy1178-2013https://doaj.org/article/4df2c196df614991b38a8cb69add9d872019-06-01T00:00:00Zhttps://www.dovepress.com/131i-labeled-polyethylenimine-entrapped-gold-nanoparticles-for-targete-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Na Sun,1,* Lingzhou Zhao,1,* Jingyi Zhu,2,* Yujie Li,1 Ningning Song,1 Yan Xing,1 Wenli Qiao,1 He Huang,2 Jinhua Zhao11Department of Nuclear Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, People’s Republic of China; 2State Key Laboratory of Material-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, People’s Republic of China*These authors contributed equally to this work Purpose: Polyethylenimine (PEI) has been widely used as a versatile template to develop multifunctional nanosystems for disease diagnosis and treatment. In this study, we manufactured iodine-131 (131I)-labeled PEI-entrapped gold nanoparticles (Au PENPs) as a novel nanoprobe for single-photon emission computed tomography/computed tomography (SPECT/CT) imaging and radionuclide therapy.Materials and methods: PEI was PEGylated and sequentially conjugated with Buthus martensii Karsch chlorotoxin (BmK CT, a tumor-specific ligand which can selectively bind to MMP2), 3-(4′-hydroxyphenyl)propionic acid-OSu (HPAO), and fluorescein isothiocyanate to form the multifunctional PEI template for entrapment of Au NPs. Then, the PEI surface was radiolabeled with 131I via HPAO to produce the novel nanoprobe (BmK CT-Au PENPs-131I).Results: The synthesized multifunctional Au PENPs before and after 131I radiolabeling were well-characterized as follows: structure, X-ray attenuation coefficient, colloid stability, cytocompatibility, and radiochemical stability in vitro. Furthermore, BmK CT-Au PENPs-131I were suitable for targeted SPECT/CT imaging and radionuclide therapy of tumor cells in vitro and in a xenograft tumor model in vivo.Conclusion: The developed multifunctional Au PENPs are a promising theranostic platform for targeted imaging and treatment of different MMP2-overexpressing tumors.Keywords: polyethylenimine, BmK CT, gold nanoparticles, SPECT/CT imaging, radionuclide therapySun NZhao LZhu JLi YSong NXing YQiao WHuang HZhao JDove Medical PressarticlePolyethylenimineBmK CTgold nanoparticlesSPECT/CT imagingradionuclide therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4367-4381 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Polyethylenimine BmK CT gold nanoparticles SPECT/CT imaging radionuclide therapy Medicine (General) R5-920
spellingShingle	Polyethylenimine BmK CT gold nanoparticles SPECT/CT imaging radionuclide therapy Medicine (General) R5-920 Sun N Zhao L Zhu J Li Y Song N Xing Y Qiao W Huang H Zhao J 131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy
description	Na Sun,1,* Lingzhou Zhao,1,* Jingyi Zhu,2,* Yujie Li,1 Ningning Song,1 Yan Xing,1 Wenli Qiao,1 He Huang,2 Jinhua Zhao11Department of Nuclear Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, People’s Republic of China; 2State Key Laboratory of Material-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, People’s Republic of China*These authors contributed equally to this work Purpose: Polyethylenimine (PEI) has been widely used as a versatile template to develop multifunctional nanosystems for disease diagnosis and treatment. In this study, we manufactured iodine-131 (131I)-labeled PEI-entrapped gold nanoparticles (Au PENPs) as a novel nanoprobe for single-photon emission computed tomography/computed tomography (SPECT/CT) imaging and radionuclide therapy.Materials and methods: PEI was PEGylated and sequentially conjugated with Buthus martensii Karsch chlorotoxin (BmK CT, a tumor-specific ligand which can selectively bind to MMP2), 3-(4′-hydroxyphenyl)propionic acid-OSu (HPAO), and fluorescein isothiocyanate to form the multifunctional PEI template for entrapment of Au NPs. Then, the PEI surface was radiolabeled with 131I via HPAO to produce the novel nanoprobe (BmK CT-Au PENPs-131I).Results: The synthesized multifunctional Au PENPs before and after 131I radiolabeling were well-characterized as follows: structure, X-ray attenuation coefficient, colloid stability, cytocompatibility, and radiochemical stability in vitro. Furthermore, BmK CT-Au PENPs-131I were suitable for targeted SPECT/CT imaging and radionuclide therapy of tumor cells in vitro and in a xenograft tumor model in vivo.Conclusion: The developed multifunctional Au PENPs are a promising theranostic platform for targeted imaging and treatment of different MMP2-overexpressing tumors.Keywords: polyethylenimine, BmK CT, gold nanoparticles, SPECT/CT imaging, radionuclide therapy
format	article
author	Sun N Zhao L Zhu J Li Y Song N Xing Y Qiao W Huang H Zhao J
author_facet	Sun N Zhao L Zhu J Li Y Song N Xing Y Qiao W Huang H Zhao J
author_sort	Sun N
title	131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy
title_short	131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy
title_full	131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy
title_fullStr	131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy
title_full_unstemmed	131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy
title_sort	131i-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor spect/ct imaging and radionuclide therapy
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/4df2c196df614991b38a8cb69add9d87
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131I-labeled polyethylenimine-entrapped gold nanoparticles for targeted tumor SPECT/CT imaging and radionuclide therapy

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