Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences

Degradation of messenger RNA is a key regulatory step in controlling eukaryotic gene expression. Here the authors present xrFrag, a molecular tool to interrogate the extent and directionality of mRNA turnover by the detection of stabilized decay intermediates produced by several common decay pathway...

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Autores principales: Volker Boehm, Jennifer V. Gerbracht, Marie-Charlotte Marx, Niels H. Gehring
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/4df3f5547cd041fa9a60258e7949e822
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