Evaluation of transplantation sites for human intestinal organoids.

Our group has developed two transplantation models for the engraftment of Human Intestinal Organoids (HIOs): the renal subcapsular space (RSS) and the mesentery each with specific benefits for study. While engraftment at both sites generates laminated intestinal structures, a direct comparison betwe...

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Autores principales:	Akaljot Singh, Holly M Poling, Nambirajan Sundaram, Nicole Brown, James M Wells, Michael A Helmrath
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4df5319f91e4406ea55d08281fbc7442
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spelling	oai:doaj.org-article:4df5319f91e4406ea55d08281fbc74422021-12-02T20:05:46ZEvaluation of transplantation sites for human intestinal organoids.1932-620310.1371/journal.pone.0237885https://doaj.org/article/4df5319f91e4406ea55d08281fbc74422020-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0237885https://doaj.org/toc/1932-6203Our group has developed two transplantation models for the engraftment of Human Intestinal Organoids (HIOs): the renal subcapsular space (RSS) and the mesentery each with specific benefits for study. While engraftment at both sites generates laminated intestinal structures, a direct comparison between models has not yet been performed. Embryonic stem cells were differentiated into HIOs, as previously described. HIOs from the same batch were transplanted on the same day into either the RSS or mesentery. 10 weeks were allowed for engraftment and differentiation, at which time they were harvested and assessed. Metrics for comparison included: mortality, engraftment rate, gross size, number and grade of lumens, and expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism. Mortality was significantly increased when undergoing mesentery transplantation, however engraftment was significantly higher. Graft sizes were similar between groups. Morphometric parameters were similar between groups, however m-tHIOs presented with significantly fewer lumens than k-tHIO. Transcript and protein level expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism were similar between groups. Transplantation into both sites yields viable tissue of similar quality based on our assessments with enhanced engraftment and a dominant lumen for uniform study benefiting the mesenteric site and survival benefiting RSS.Akaljot SinghHolly M PolingNambirajan SundaramNicole BrownJames M WellsMichael A HelmrathPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 15, Iss 8, p e0237885 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akaljot Singh Holly M Poling Nambirajan Sundaram Nicole Brown James M Wells Michael A Helmrath Evaluation of transplantation sites for human intestinal organoids.
description	Our group has developed two transplantation models for the engraftment of Human Intestinal Organoids (HIOs): the renal subcapsular space (RSS) and the mesentery each with specific benefits for study. While engraftment at both sites generates laminated intestinal structures, a direct comparison between models has not yet been performed. Embryonic stem cells were differentiated into HIOs, as previously described. HIOs from the same batch were transplanted on the same day into either the RSS or mesentery. 10 weeks were allowed for engraftment and differentiation, at which time they were harvested and assessed. Metrics for comparison included: mortality, engraftment rate, gross size, number and grade of lumens, and expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism. Mortality was significantly increased when undergoing mesentery transplantation, however engraftment was significantly higher. Graft sizes were similar between groups. Morphometric parameters were similar between groups, however m-tHIOs presented with significantly fewer lumens than k-tHIO. Transcript and protein level expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism were similar between groups. Transplantation into both sites yields viable tissue of similar quality based on our assessments with enhanced engraftment and a dominant lumen for uniform study benefiting the mesenteric site and survival benefiting RSS.
format	article
author	Akaljot Singh Holly M Poling Nambirajan Sundaram Nicole Brown James M Wells Michael A Helmrath
author_facet	Akaljot Singh Holly M Poling Nambirajan Sundaram Nicole Brown James M Wells Michael A Helmrath
author_sort	Akaljot Singh
title	Evaluation of transplantation sites for human intestinal organoids.
title_short	Evaluation of transplantation sites for human intestinal organoids.
title_full	Evaluation of transplantation sites for human intestinal organoids.
title_fullStr	Evaluation of transplantation sites for human intestinal organoids.
title_full_unstemmed	Evaluation of transplantation sites for human intestinal organoids.
title_sort	evaluation of transplantation sites for human intestinal organoids.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/4df5319f91e4406ea55d08281fbc7442
work_keys_str_mv	AT akaljotsingh evaluationoftransplantationsitesforhumanintestinalorganoids AT hollympoling evaluationoftransplantationsitesforhumanintestinalorganoids AT nambirajansundaram evaluationoftransplantationsitesforhumanintestinalorganoids AT nicolebrown evaluationoftransplantationsitesforhumanintestinalorganoids AT jamesmwells evaluationoftransplantationsitesforhumanintestinalorganoids AT michaelahelmrath evaluationoftransplantationsitesforhumanintestinalorganoids
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Evaluation of transplantation sites for human intestinal organoids.

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