Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

CRISPR-Cas9 has been utilized to screen the genome in loss-of-function studies to identify genetic interactions. Here the authors compare catalytically active and dead Cas9 and observe different off-target effects in a screen for essential genes.

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Autores principales:	Joseph Rosenbluh, Han Xu, William Harrington, Stanley Gill, Xiaoxing Wang, Francisca Vazquez, David E. Root, Aviad Tsherniak, William C. Hahn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4df7e335ce2740e5852e4891179365f8
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spelling	oai:doaj.org-article:4df7e335ce2740e5852e4891179365f82021-12-02T14:42:04ZComplementary information derived from CRISPR Cas9 mediated gene deletion and suppression10.1038/ncomms154032041-1723https://doaj.org/article/4df7e335ce2740e5852e4891179365f82017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15403https://doaj.org/toc/2041-1723CRISPR-Cas9 has been utilized to screen the genome in loss-of-function studies to identify genetic interactions. Here the authors compare catalytically active and dead Cas9 and observe different off-target effects in a screen for essential genes.Joseph RosenbluhHan XuWilliam HarringtonStanley GillXiaoxing WangFrancisca VazquezDavid E. RootAviad TsherniakWilliam C. HahnNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Joseph Rosenbluh Han Xu William Harrington Stanley Gill Xiaoxing Wang Francisca Vazquez David E. Root Aviad Tsherniak William C. Hahn Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
description	CRISPR-Cas9 has been utilized to screen the genome in loss-of-function studies to identify genetic interactions. Here the authors compare catalytically active and dead Cas9 and observe different off-target effects in a screen for essential genes.
format	article
author	Joseph Rosenbluh Han Xu William Harrington Stanley Gill Xiaoxing Wang Francisca Vazquez David E. Root Aviad Tsherniak William C. Hahn
author_facet	Joseph Rosenbluh Han Xu William Harrington Stanley Gill Xiaoxing Wang Francisca Vazquez David E. Root Aviad Tsherniak William C. Hahn
author_sort	Joseph Rosenbluh
title	Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
title_short	Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
title_full	Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
title_fullStr	Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
title_full_unstemmed	Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
title_sort	complementary information derived from crispr cas9 mediated gene deletion and suppression
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4df7e335ce2740e5852e4891179365f8
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Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

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