Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice

Vaccination strategies for rapid protection against multidrug-resistant bacterial infection are very important, especially for hospitalized patients who have high risk of exposure to these bacteria. However, few such vaccination strategies exist due to a shortage of knowledge supporting their rapid...

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Autores principales:	Hao Gu, Xi Zeng, Liusheng Peng, Chuanying Xiang, Yangyang Zhou, Xiaomin Zhang, Jixin Zhang, Ning Wang, Gang Guo, Yan Li, Kaiyun Liu, Jiang Gu, Hao Zeng, Yuan Zhuang, Haibo Li, Jinyong Zhang, Weijun Zhang, Quanming Zou, Yun Shi
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	multidrug-resistant bacteria vaccine alveolar macrophage Trained immunity Acinetobacter baumannii rapid effect Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4dfda56e312643cf88f336477e954a50
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spelling	oai:doaj.org-article:4dfda56e312643cf88f336477e954a502021-11-30T10:05:54ZVaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice10.7554/eLife.699512050-084Xe69951https://doaj.org/article/4dfda56e312643cf88f336477e954a502021-09-01T00:00:00Zhttps://elifesciences.org/articles/69951https://doaj.org/toc/2050-084XVaccination strategies for rapid protection against multidrug-resistant bacterial infection are very important, especially for hospitalized patients who have high risk of exposure to these bacteria. However, few such vaccination strategies exist due to a shortage of knowledge supporting their rapid effect. Here, we demonstrated that a single intranasal immunization of inactivated whole cell of Acinetobacter baumannii elicits rapid protection against broad A. baumannii-infected pneumonia via training of innate immune response in Rag1-/- mice. Immunization-trained alveolar macrophages (AMs) showed enhanced TNF-α production upon restimulation. Adoptive transfer of immunization-trained AMs into naive mice mediated rapid protection against infection. Elevated TLR4 expression on vaccination-trained AMs contributed to rapid protection. Moreover, immunization-induced rapid protection was also seen in Pseudomonas aeruginosa and Klebsiella pneumoniae pneumonia models, but not in Staphylococcus aureus and Streptococcus pneumoniae model. Our data reveal that a single intranasal immunization induces rapid and efficient protection against certain Gram-negative bacterial pneumonia via training AMs response, which highlights the importance and the possibility of harnessing trained immunity of AMs to design rapid-effecting vaccine.Hao GuXi ZengLiusheng PengChuanying XiangYangyang ZhouXiaomin ZhangJixin ZhangNing WangGang GuoYan LiKaiyun LiuJiang GuHao ZengYuan ZhuangHaibo LiJinyong ZhangWeijun ZhangQuanming ZouYun ShieLife Sciences Publications Ltdarticlemultidrug-resistant bacteriavaccinealveolar macrophageTrained immunityAcinetobacter baumanniirapid effectMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	multidrug-resistant bacteria vaccine alveolar macrophage Trained immunity Acinetobacter baumannii rapid effect Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	multidrug-resistant bacteria vaccine alveolar macrophage Trained immunity Acinetobacter baumannii rapid effect Medicine R Science Q Biology (General) QH301-705.5 Hao Gu Xi Zeng Liusheng Peng Chuanying Xiang Yangyang Zhou Xiaomin Zhang Jixin Zhang Ning Wang Gang Guo Yan Li Kaiyun Liu Jiang Gu Hao Zeng Yuan Zhuang Haibo Li Jinyong Zhang Weijun Zhang Quanming Zou Yun Shi Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice
description	Vaccination strategies for rapid protection against multidrug-resistant bacterial infection are very important, especially for hospitalized patients who have high risk of exposure to these bacteria. However, few such vaccination strategies exist due to a shortage of knowledge supporting their rapid effect. Here, we demonstrated that a single intranasal immunization of inactivated whole cell of Acinetobacter baumannii elicits rapid protection against broad A. baumannii-infected pneumonia via training of innate immune response in Rag1-/- mice. Immunization-trained alveolar macrophages (AMs) showed enhanced TNF-α production upon restimulation. Adoptive transfer of immunization-trained AMs into naive mice mediated rapid protection against infection. Elevated TLR4 expression on vaccination-trained AMs contributed to rapid protection. Moreover, immunization-induced rapid protection was also seen in Pseudomonas aeruginosa and Klebsiella pneumoniae pneumonia models, but not in Staphylococcus aureus and Streptococcus pneumoniae model. Our data reveal that a single intranasal immunization induces rapid and efficient protection against certain Gram-negative bacterial pneumonia via training AMs response, which highlights the importance and the possibility of harnessing trained immunity of AMs to design rapid-effecting vaccine.
format	article
author	Hao Gu Xi Zeng Liusheng Peng Chuanying Xiang Yangyang Zhou Xiaomin Zhang Jixin Zhang Ning Wang Gang Guo Yan Li Kaiyun Liu Jiang Gu Hao Zeng Yuan Zhuang Haibo Li Jinyong Zhang Weijun Zhang Quanming Zou Yun Shi
author_facet	Hao Gu Xi Zeng Liusheng Peng Chuanying Xiang Yangyang Zhou Xiaomin Zhang Jixin Zhang Ning Wang Gang Guo Yan Li Kaiyun Liu Jiang Gu Hao Zeng Yuan Zhuang Haibo Li Jinyong Zhang Weijun Zhang Quanming Zou Yun Shi
author_sort	Hao Gu
title	Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice
title_short	Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice
title_full	Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice
title_fullStr	Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice
title_full_unstemmed	Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice
title_sort	vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/4dfda56e312643cf88f336477e954a50
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Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice

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