Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood
Conjugated linoleic acid (CLA) isomers are potent health-promoting fatty acids. This study evaluated the probiotic properties of 10 strains of high CLA-producing lactic acid bacteria (LAB) isolated from <i>Jeot-gal</i>, a high-salt, fermented seafood. Two isolates, <i>Lactiplantiba...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/4dffbbecf957431fa8349f3670d97b62 |
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| Sumario: | Conjugated linoleic acid (CLA) isomers are potent health-promoting fatty acids. This study evaluated the probiotic properties of 10 strains of high CLA-producing lactic acid bacteria (LAB) isolated from <i>Jeot-gal</i>, a high-salt, fermented seafood. Two isolates, <i>Lactiplantibacillus plantarum</i> JBCC105683 and <i>Lactiplantibacillus pentosus</i> JBCC105676, produced the largest amounts of CLA (748.8 and 726.9 μg/mL, respectively). Five isolates, <i>L. plantarum</i> JBCC105675, <i>L. pentosus</i> JBCC105676, <i>L. pentosus</i> JBCC105674, <i>L. plantarum</i> JBCC105683, and <i>Lactiplantibacillus paraplantarum</i> JBCC105655 synthesized more <i>cis</i>-9, <i>trans</i>-11-CLA than <i>trans</i>-10, <i>cis</i>-12-CLA (approximately 80:20 ratio). All the strains survived severe artificial acidic environments and showed antimicrobial activity and strong adhesion capability to Caco-2 cells as compared to the commercial strain <i>Lactocaseibacillus rhamnosus</i> GG. Among them, <i>Pediococcus acidilactici</i> JBCC105117, <i>L. paraplantarum</i> JBCC105655, and <i>L. plantarum</i> JBCC105683 strongly stimulated the immunological regulatory gene PMK-1 and the host defense antimicrobial peptide gene <i>clec-60</i> in <i>Caenorhabditis elegans.</i> Moreover, three strains showed a significant induction of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-12, and IL-10 production in RAW 264.7 macrophages, indicating that they were promising candidates for probiotics with high CLA-converting activity. Our results indicate that the newly isolated CLA-producing LAB might be useful as a functional probiotic with beneficial health effects that modulate the immune system. |
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