Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from Jeot-Gal, High-Salt Fermented Seafood

Conjugated linoleic acid (CLA) isomers are potent health-promoting fatty acids. This study evaluated the probiotic properties of 10 strains of high CLA-producing lactic acid bacteria (LAB) isolated from Jeot-gal, a high-salt, fermented seafood. Two isolates, Lactiplantiba...

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Autores principales:	Nho-Eul Song, Na-Jeong Kim, Young-Hun Kim, Sang-Ho Baik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	conjugated linoleic acid lactic acid bacteria <i>Jeot-gal</i>s probiotics linoleic acid isomerase Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4dffbbecf957431fa8349f3670d97b62
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spelling	oai:doaj.org-article:4dffbbecf957431fa8349f3670d97b622021-11-25T18:24:35ZProbiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood10.3390/microorganisms91122472076-2607https://doaj.org/article/4dffbbecf957431fa8349f3670d97b622021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2247https://doaj.org/toc/2076-2607Conjugated linoleic acid (CLA) isomers are potent health-promoting fatty acids. This study evaluated the probiotic properties of 10 strains of high CLA-producing lactic acid bacteria (LAB) isolated from <i>Jeot-gal</i>, a high-salt, fermented seafood. Two isolates, <i>Lactiplantibacillus plantarum</i> JBCC105683 and <i>Lactiplantibacillus pentosus</i> JBCC105676, produced the largest amounts of CLA (748.8 and 726.9 μg/mL, respectively). Five isolates, <i>L. plantarum</i> JBCC105675, <i>L. pentosus</i> JBCC105676, <i>L. pentosus</i> JBCC105674, <i>L. plantarum</i> JBCC105683, and <i>Lactiplantibacillus paraplantarum</i> JBCC105655 synthesized more <i>cis</i>-9, <i>trans</i>-11-CLA than <i>trans</i>-10, <i>cis</i>-12-CLA (approximately 80:20 ratio). All the strains survived severe artificial acidic environments and showed antimicrobial activity and strong adhesion capability to Caco-2 cells as compared to the commercial strain <i>Lactocaseibacillus rhamnosus</i> GG. Among them, <i>Pediococcus acidilactici</i> JBCC105117, <i>L. paraplantarum</i> JBCC105655, and <i>L. plantarum</i> JBCC105683 strongly stimulated the immunological regulatory gene PMK-1 and the host defense antimicrobial peptide gene <i>clec-60</i> in <i>Caenorhabditis elegans.</i> Moreover, three strains showed a significant induction of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-12, and IL-10 production in RAW 264.7 macrophages, indicating that they were promising candidates for probiotics with high CLA-converting activity. Our results indicate that the newly isolated CLA-producing LAB might be useful as a functional probiotic with beneficial health effects that modulate the immune system.Nho-Eul SongNa-Jeong KimYoung-Hun KimSang-Ho BaikMDPI AGarticleconjugated linoleic acidlactic acid bacteria<i>Jeot-gal</i>sprobioticslinoleic acid isomeraseBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2247, p 2247 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	conjugated linoleic acid lactic acid bacteria <i>Jeot-gal</i>s probiotics linoleic acid isomerase Biology (General) QH301-705.5
spellingShingle	conjugated linoleic acid lactic acid bacteria <i>Jeot-gal</i>s probiotics linoleic acid isomerase Biology (General) QH301-705.5 Nho-Eul Song Na-Jeong Kim Young-Hun Kim Sang-Ho Baik Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood
description	Conjugated linoleic acid (CLA) isomers are potent health-promoting fatty acids. This study evaluated the probiotic properties of 10 strains of high CLA-producing lactic acid bacteria (LAB) isolated from <i>Jeot-gal</i>, a high-salt, fermented seafood. Two isolates, <i>Lactiplantibacillus plantarum</i> JBCC105683 and <i>Lactiplantibacillus pentosus</i> JBCC105676, produced the largest amounts of CLA (748.8 and 726.9 μg/mL, respectively). Five isolates, <i>L. plantarum</i> JBCC105675, <i>L. pentosus</i> JBCC105676, <i>L. pentosus</i> JBCC105674, <i>L. plantarum</i> JBCC105683, and <i>Lactiplantibacillus paraplantarum</i> JBCC105655 synthesized more <i>cis</i>-9, <i>trans</i>-11-CLA than <i>trans</i>-10, <i>cis</i>-12-CLA (approximately 80:20 ratio). All the strains survived severe artificial acidic environments and showed antimicrobial activity and strong adhesion capability to Caco-2 cells as compared to the commercial strain <i>Lactocaseibacillus rhamnosus</i> GG. Among them, <i>Pediococcus acidilactici</i> JBCC105117, <i>L. paraplantarum</i> JBCC105655, and <i>L. plantarum</i> JBCC105683 strongly stimulated the immunological regulatory gene PMK-1 and the host defense antimicrobial peptide gene <i>clec-60</i> in <i>Caenorhabditis elegans.</i> Moreover, three strains showed a significant induction of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-12, and IL-10 production in RAW 264.7 macrophages, indicating that they were promising candidates for probiotics with high CLA-converting activity. Our results indicate that the newly isolated CLA-producing LAB might be useful as a functional probiotic with beneficial health effects that modulate the immune system.
format	article
author	Nho-Eul Song Na-Jeong Kim Young-Hun Kim Sang-Ho Baik
author_facet	Nho-Eul Song Na-Jeong Kim Young-Hun Kim Sang-Ho Baik
author_sort	Nho-Eul Song
title	Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood
title_short	Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood
title_full	Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood
title_fullStr	Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood
title_full_unstemmed	Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood
title_sort	probiotic properties of lactic acid bacteria with high conjugated linoleic acid converting activity isolated from <i>jeot-gal</i>, high-salt fermented seafood
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4dffbbecf957431fa8349f3670d97b62
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Probiotic Properties of Lactic Acid Bacteria with High Conjugated Linoleic Acid Converting Activity Isolated from <i>Jeot-Gal</i>, High-Salt Fermented Seafood

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