Upregulation of vitamin D-related genes in schizophrenic patients

Fateme Asadzadeh Manjili,1 Seyed Mehdi Kalantar,2 Shahram Arsang-Jang,3 Soudeh Ghafouri-Fard,4 Mohammad Taheri,4,5 Arezou Sayad4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Reproductive and Genetic Unit, Recurrent Abortion Research Center, Yazd Repr...

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Autores principales:	Asadzadeh Manjili F, Kalantar SM, Arsang-Jang S, Ghafouri-Fard S, Taheri M, Sayad A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	CYP27B1 CYP24A1 VDR schizophrenia Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/4e0000c691684a25987fd214b445ea90
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spelling	oai:doaj.org-article:4e0000c691684a25987fd214b445ea902021-12-02T04:38:35ZUpregulation of vitamin D-related genes in schizophrenic patients1178-2021https://doaj.org/article/4e0000c691684a25987fd214b445ea902018-10-01T00:00:00Zhttps://www.dovepress.com/upregulation-of-vitamin-d-related-genes-in-schizophrenic-patients-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Fateme Asadzadeh Manjili,1 Seyed Mehdi Kalantar,2 Shahram Arsang-Jang,3 Soudeh Ghafouri-Fard,4 Mohammad Taheri,4,5 Arezou Sayad4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Reproductive and Genetic Unit, Recurrent Abortion Research Center, Yazd Reproductive Science Institute, Yazd University of Medical Sciences, Yazd, Iran; 3Clinical Research Development Center (CRDU), Qom University of Medical Sciences, Qom, Iran; 4Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Introduction: Low level of vitamin D is a potential risk factor for developing schizophrenia. Through interaction with its receptor (VDR) and the related enzymes (CYP27B1, CYP24A1), vitamin D modulates neurodevelopment, neuroprotection, and immunomodulation. Its deficiency leads to aberrant neurodevelopment in schizophrenic patients. Methods: In this case–control study, relative expression of VDR, CYP27B1, and CYP24A1 in schizophrenic patients was compared with healthy individuals. Total RNA was extracted from whole blood of 50 patients with schizophrenia and 50 healthy controls. Real-time PCR was used to determine relative gene expression levels of VDR, CYP27B1, and CYP24A1. Results: Significant upregulations were observed in VDR (P=0.004, 95% CI=0.77, 0.86), CYP27B1 (P=0.002, 95% CI=1.22, 4.98), and CYP24A1 (P≤0.0001, 95% CI=-2.721, 1.061) expressions in peripheral blood of schizophrenic patients compared with controls. Moreover, the gender-based analysis revealed upregulation of all genes in all the categories of male and female except for VDR gene in male group (P=0.234, 95% CI=-0.79, 3.35) and CYP27B1 gene in the female group (P=0.09, 95% CI=-0.21, 6.55). The age-based analysis demonstrated overexpression of VDR and CYP27B1 genes in all categories. Finally, there were significant correlations between expression levels of all genes (P<0.0001), while no correlation was found between age and expression of genes. Conclusion: We hypothesized that the observed upregulation of the mentioned genes in schizophrenia patients might be the result of a compensatory mechanism to protect the affected individuals against adverse consequences of this disorder. Such imbalance in vitamin D processing pathway might also be implicated in the pathogenesis of schizophrenia. However, future studies should be designed to confirm the results of the current study. Keywords: CYP27B1, CYP24A1, VDR, schizophreniaAsadzadeh Manjili FKalantar SMArsang-Jang SGhafouri-Fard STaheri MSayad ADove Medical PressarticleCYP27B1CYP24A1VDRschizophreniaNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 14, Pp 2583-2591 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	CYP27B1 CYP24A1 VDR schizophrenia Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	CYP27B1 CYP24A1 VDR schizophrenia Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Asadzadeh Manjili F Kalantar SM Arsang-Jang S Ghafouri-Fard S Taheri M Sayad A Upregulation of vitamin D-related genes in schizophrenic patients
description	Fateme Asadzadeh Manjili,1 Seyed Mehdi Kalantar,2 Shahram Arsang-Jang,3 Soudeh Ghafouri-Fard,4 Mohammad Taheri,4,5 Arezou Sayad4 1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Reproductive and Genetic Unit, Recurrent Abortion Research Center, Yazd Reproductive Science Institute, Yazd University of Medical Sciences, Yazd, Iran; 3Clinical Research Development Center (CRDU), Qom University of Medical Sciences, Qom, Iran; 4Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Introduction: Low level of vitamin D is a potential risk factor for developing schizophrenia. Through interaction with its receptor (VDR) and the related enzymes (CYP27B1, CYP24A1), vitamin D modulates neurodevelopment, neuroprotection, and immunomodulation. Its deficiency leads to aberrant neurodevelopment in schizophrenic patients. Methods: In this case–control study, relative expression of VDR, CYP27B1, and CYP24A1 in schizophrenic patients was compared with healthy individuals. Total RNA was extracted from whole blood of 50 patients with schizophrenia and 50 healthy controls. Real-time PCR was used to determine relative gene expression levels of VDR, CYP27B1, and CYP24A1. Results: Significant upregulations were observed in VDR (P=0.004, 95% CI=0.77, 0.86), CYP27B1 (P=0.002, 95% CI=1.22, 4.98), and CYP24A1 (P≤0.0001, 95% CI=-2.721, 1.061) expressions in peripheral blood of schizophrenic patients compared with controls. Moreover, the gender-based analysis revealed upregulation of all genes in all the categories of male and female except for VDR gene in male group (P=0.234, 95% CI=-0.79, 3.35) and CYP27B1 gene in the female group (P=0.09, 95% CI=-0.21, 6.55). The age-based analysis demonstrated overexpression of VDR and CYP27B1 genes in all categories. Finally, there were significant correlations between expression levels of all genes (P<0.0001), while no correlation was found between age and expression of genes. Conclusion: We hypothesized that the observed upregulation of the mentioned genes in schizophrenia patients might be the result of a compensatory mechanism to protect the affected individuals against adverse consequences of this disorder. Such imbalance in vitamin D processing pathway might also be implicated in the pathogenesis of schizophrenia. However, future studies should be designed to confirm the results of the current study. Keywords: CYP27B1, CYP24A1, VDR, schizophrenia
format	article
author	Asadzadeh Manjili F Kalantar SM Arsang-Jang S Ghafouri-Fard S Taheri M Sayad A
author_facet	Asadzadeh Manjili F Kalantar SM Arsang-Jang S Ghafouri-Fard S Taheri M Sayad A
author_sort	Asadzadeh Manjili F
title	Upregulation of vitamin D-related genes in schizophrenic patients
title_short	Upregulation of vitamin D-related genes in schizophrenic patients
title_full	Upregulation of vitamin D-related genes in schizophrenic patients
title_fullStr	Upregulation of vitamin D-related genes in schizophrenic patients
title_full_unstemmed	Upregulation of vitamin D-related genes in schizophrenic patients
title_sort	upregulation of vitamin d-related genes in schizophrenic patients
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/4e0000c691684a25987fd214b445ea90
work_keys_str_mv	AT asadzadehmanjilif upregulationofvitamindrelatedgenesinschizophrenicpatients AT kalantarsm upregulationofvitamindrelatedgenesinschizophrenicpatients AT arsangjangs upregulationofvitamindrelatedgenesinschizophrenicpatients AT ghafourifards upregulationofvitamindrelatedgenesinschizophrenicpatients AT taherim upregulationofvitamindrelatedgenesinschizophrenicpatients AT sayada upregulationofvitamindrelatedgenesinschizophrenicpatients
_version_	1718401118568972288

Upregulation of vitamin D-related genes in schizophrenic patients

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