Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.

A Resequencing Pathogen Microarray (RPM) is a single, highly multiplexed assay for detecting and differentiating similarly related pathogens by using closely overlapping probe sets to determine a target organism's nucleotide sequence. In this study, a new RPM (RPM-IVDC1) that consisted of 224-b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hongwei Shen, Weixian Shi, Ji Wang, Miao Wang, Jin Li, Chen Zhang, Kai Nie, Mengjie Yang, Yi Zhang, Aihua Li, Wenjie Tan, Xuejun Ma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e04706415e84ed189932b1cd3154d8d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4e04706415e84ed189932b1cd3154d8d
record_format	dspace
spelling	oai:doaj.org-article:4e04706415e84ed189932b1cd3154d8d2021-11-18T08:53:29ZDevelopment of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.1932-620310.1371/journal.pone.0075704https://doaj.org/article/4e04706415e84ed189932b1cd3154d8d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086618/?tool=EBIhttps://doaj.org/toc/1932-6203A Resequencing Pathogen Microarray (RPM) is a single, highly multiplexed assay for detecting and differentiating similarly related pathogens by using closely overlapping probe sets to determine a target organism's nucleotide sequence. In this study, a new RPM (RPM-IVDC1) that consisted of 224-bp detector tiles corresponding to 9 influenza A subtypes, 11 rhinoviruses, 28 enteroviruses and 38 other respiratory viruses was developed and optimized to provide individual and simultaneous detection sensitivities ranging from 15 to 750 genomic copies for 16 common respiratory pathogens. A total of 110 consecutive patients with community-acquired pneumonia (CAP) admitted to 5 district general hospitals in Beijing during a 1-year period were assessed using the new assay. Among the children (under age 5) and adult patients (above age 18), respiratory syncytial virus (RSV) and rhinovirus (RV) were the most common etiological agents, respectively, which is consistent with reference assays. Atypical pathogens that may cause CAP-like illness, including rubella virus, measles virus, influenza type C virus, human herpesvirus (HHV) were also detected. The results show the capability of RPM-IVDC1 for the accurate detection and identification of multiple virus types, which may be of significant use in epidemic surveillance and outbreak investigations of atypical pathogens.Hongwei ShenWeixian ShiJi WangMiao WangJin LiChen ZhangKai NieMengjie YangYi ZhangAihua LiWenjie TanXuejun MaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75704 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongwei Shen Weixian Shi Ji Wang Miao Wang Jin Li Chen Zhang Kai Nie Mengjie Yang Yi Zhang Aihua Li Wenjie Tan Xuejun Ma Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.
description	A Resequencing Pathogen Microarray (RPM) is a single, highly multiplexed assay for detecting and differentiating similarly related pathogens by using closely overlapping probe sets to determine a target organism's nucleotide sequence. In this study, a new RPM (RPM-IVDC1) that consisted of 224-bp detector tiles corresponding to 9 influenza A subtypes, 11 rhinoviruses, 28 enteroviruses and 38 other respiratory viruses was developed and optimized to provide individual and simultaneous detection sensitivities ranging from 15 to 750 genomic copies for 16 common respiratory pathogens. A total of 110 consecutive patients with community-acquired pneumonia (CAP) admitted to 5 district general hospitals in Beijing during a 1-year period were assessed using the new assay. Among the children (under age 5) and adult patients (above age 18), respiratory syncytial virus (RSV) and rhinovirus (RV) were the most common etiological agents, respectively, which is consistent with reference assays. Atypical pathogens that may cause CAP-like illness, including rubella virus, measles virus, influenza type C virus, human herpesvirus (HHV) were also detected. The results show the capability of RPM-IVDC1 for the accurate detection and identification of multiple virus types, which may be of significant use in epidemic surveillance and outbreak investigations of atypical pathogens.
format	article
author	Hongwei Shen Weixian Shi Ji Wang Miao Wang Jin Li Chen Zhang Kai Nie Mengjie Yang Yi Zhang Aihua Li Wenjie Tan Xuejun Ma
author_facet	Hongwei Shen Weixian Shi Ji Wang Miao Wang Jin Li Chen Zhang Kai Nie Mengjie Yang Yi Zhang Aihua Li Wenjie Tan Xuejun Ma
author_sort	Hongwei Shen
title	Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.
title_short	Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.
title_full	Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.
title_fullStr	Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.
title_full_unstemmed	Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.
title_sort	development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4e04706415e84ed189932b1cd3154d8d
work_keys_str_mv	AT hongweishen developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT weixianshi developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT jiwang developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT miaowang developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT jinli developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT chenzhang developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT kainie developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT mengjieyang developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT yizhang developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT aihuali developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT wenjietan developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia AT xuejunma developmentofanewresequencingpathogenmicroarraybasedassayfordetectionofbroadspectrumrespiratorytractvirusesinpatientswithcommunityacquiredpneumonia
_version_	1718421199222996992

Development of a new resequencing pathogen microarray based assay for detection of broad-spectrum respiratory tract viruses in patients with community-acquired pneumonia.

Ejemplares similares