Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4

Xianglei Fu,1 Yanbin Shi,2 Hui Wang,1 Xiaogang Zhao,3 Qifeng Sun,3 Yi Huang,1 Tongtong Qi,1 Guimei Lin1 1School of Pharmaceutical Science, Shandong University, Jinan 250012,People’s Republic of China; 2School of Mechanical & Automotive Engineering, Qilu University of Technology (Sh...

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Autores principales:	Fu X, Shi Y, Wang H, Zhao X, Sun Q, Huang Y, Qi T, Lin G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	ethosomes transdermal drug delivery system macromolecular protein drugs skin wound healing Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4e0a5b8faaa3432ea0d02389cb1b2b23
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spelling	oai:doaj.org-article:4e0a5b8faaa3432ea0d02389cb1b2b232021-12-02T04:38:01ZEthosomal Gel for Improving Transdermal Delivery of Thymosin β-41178-2013https://doaj.org/article/4e0a5b8faaa3432ea0d02389cb1b2b232019-11-01T00:00:00Zhttps://www.dovepress.com/ethosomal-gel-for-improving-transdermal-delivery-of-thymosin-beta-4-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xianglei Fu,1 Yanbin Shi,2 Hui Wang,1 Xiaogang Zhao,3 Qifeng Sun,3 Yi Huang,1 Tongtong Qi,1 Guimei Lin1 1School of Pharmaceutical Science, Shandong University, Jinan 250012,People’s Republic of China; 2School of Mechanical & Automotive Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People’s Republic of China; 3The Second Hospital of Shandong University, Jinan 250033, People’s Republic of ChinaCorrespondence: Guimei LinSchool of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan 250012, People’s Republic of ChinaTel +86-531- 88382548Fax +86-531-89631517Email guimeilin@sdu.edu.cnPurpose: Thymosin β-4(Tβ-4) is a macromolecular protein drug with potential for drug development in wound repair but is limited by the shortcomings of macromolecular protein, such as large volumes, poor membrane permeability, and unstable physicochemical characteristics. Ethosomes could enhance cell membrane fluidity and reduce epidermal membrane density to make macromolecular drugs through the stratum corneum into the deeper layers of the skin easily. Herein, we developed and characterized a novel transdermal delivery vehicle to load macromolecular protein peptides and use Tβ-4 as a model drug wrapped into ethosomes.Methods: We used the orthogonal method to optimize the formulation of the ethosome preparation prepared by the ethonal infusion method. Ethosomal gels were characterized by using different analytical methods. Transdermal release rate in vitro have been demonstrated in Franz diffusion cells and the efficacy of drug-loaded nanocarriers in vivo was investigated in a mouse model.Results: Optimized Tβ-4 ethosomal gels have good physicochemical properties. The drug amounts of the cumulative release in the ethosomal gel within 5 hours were 1.67 times that of the T-β4 gel in vitro release study, and the wound healing time of ethosomal gel group was only half of the T-β4 gel group in vivo pharmacokinetic study. Compared with the free drug group, the ethosome preparation not only promotes the percutaneous absorption process of the macromolecular protein drugs but also shortened wound recovery time.Conclusion: Hence, we provide a possible good design for ethosomal gel system that can load macromolecular protein peptide drugs to achieve transdermal drug administration, promoting the percutaneous absorption of the drug and improving the effect.Keywords: ethosomes, transdermal drug delivery system, macromolecular protein drugs, skin wound healingFu XShi YWang HZhao XSun QHuang YQi TLin GDove Medical Pressarticleethosomestransdermal drug delivery systemmacromolecular protein drugsskin wound healingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9275-9284 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ethosomes transdermal drug delivery system macromolecular protein drugs skin wound healing Medicine (General) R5-920
spellingShingle	ethosomes transdermal drug delivery system macromolecular protein drugs skin wound healing Medicine (General) R5-920 Fu X Shi Y Wang H Zhao X Sun Q Huang Y Qi T Lin G Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4
description	Xianglei Fu,1 Yanbin Shi,2 Hui Wang,1 Xiaogang Zhao,3 Qifeng Sun,3 Yi Huang,1 Tongtong Qi,1 Guimei Lin1 1School of Pharmaceutical Science, Shandong University, Jinan 250012,People’s Republic of China; 2School of Mechanical & Automotive Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People’s Republic of China; 3The Second Hospital of Shandong University, Jinan 250033, People’s Republic of ChinaCorrespondence: Guimei LinSchool of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan 250012, People’s Republic of ChinaTel +86-531- 88382548Fax +86-531-89631517Email guimeilin@sdu.edu.cnPurpose: Thymosin β-4(Tβ-4) is a macromolecular protein drug with potential for drug development in wound repair but is limited by the shortcomings of macromolecular protein, such as large volumes, poor membrane permeability, and unstable physicochemical characteristics. Ethosomes could enhance cell membrane fluidity and reduce epidermal membrane density to make macromolecular drugs through the stratum corneum into the deeper layers of the skin easily. Herein, we developed and characterized a novel transdermal delivery vehicle to load macromolecular protein peptides and use Tβ-4 as a model drug wrapped into ethosomes.Methods: We used the orthogonal method to optimize the formulation of the ethosome preparation prepared by the ethonal infusion method. Ethosomal gels were characterized by using different analytical methods. Transdermal release rate in vitro have been demonstrated in Franz diffusion cells and the efficacy of drug-loaded nanocarriers in vivo was investigated in a mouse model.Results: Optimized Tβ-4 ethosomal gels have good physicochemical properties. The drug amounts of the cumulative release in the ethosomal gel within 5 hours were 1.67 times that of the T-β4 gel in vitro release study, and the wound healing time of ethosomal gel group was only half of the T-β4 gel group in vivo pharmacokinetic study. Compared with the free drug group, the ethosome preparation not only promotes the percutaneous absorption process of the macromolecular protein drugs but also shortened wound recovery time.Conclusion: Hence, we provide a possible good design for ethosomal gel system that can load macromolecular protein peptide drugs to achieve transdermal drug administration, promoting the percutaneous absorption of the drug and improving the effect.Keywords: ethosomes, transdermal drug delivery system, macromolecular protein drugs, skin wound healing
format	article
author	Fu X Shi Y Wang H Zhao X Sun Q Huang Y Qi T Lin G
author_facet	Fu X Shi Y Wang H Zhao X Sun Q Huang Y Qi T Lin G
author_sort	Fu X
title	Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4
title_short	Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4
title_full	Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4
title_fullStr	Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4
title_full_unstemmed	Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4
title_sort	ethosomal gel for improving transdermal delivery of thymosin β-4
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/4e0a5b8faaa3432ea0d02389cb1b2b23
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Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4

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