Effects of a micro/nano rough strontium-loaded surface on osseointegration

Yongfeng Li,1,* Yaping Qi,2,* Qi Gao,3,* Qiang Niu,1 Mingming Shen,2 Qian Fu,1 Kaijin Hu,1 Liang Kong1 1State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi’an, 2Department of Oral and...

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Autores principales:	Li Y, Qi Y, Gao Q, Niu Q, Shen M, Fu Q, Hu K, Kong L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4e0c9147c7ec49af8971f5eef1846038
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spelling	oai:doaj.org-article:4e0c9147c7ec49af8971f5eef18460382021-12-02T02:32:06ZEffects of a micro/nano rough strontium-loaded surface on osseointegration1178-2013https://doaj.org/article/4e0c9147c7ec49af8971f5eef18460382015-07-01T00:00:00Zhttp://www.dovepress.com/effects-of-a-micronano-rough-strontium-loaded-surface-on-osseointegrat-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yongfeng Li,1,* Yaping Qi,2,* Qi Gao,3,* Qiang Niu,1 Mingming Shen,2 Qian Fu,1 Kaijin Hu,1 Liang Kong1 1State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi’an, 2Department of Oral and Maxillofacial Surgery, School of Stomatology, the Second Hospital of Hebei Medical University, Shijiazhuang, 3Department of Stomatology, PLA 458 Hospital, Guangzhou, People’s Republic of China *These authors contributed equally to this work Abstract: We developed a hierarchical hybrid micro/nanorough strontium-loaded Ti (MNT-Sr) surface fabricated through hydrofluoric acid etching followed by magnetron sputtering and evaluated the effects of this surface on osseointegration. Samples with a smooth Ti (ST) surface, micro Ti (MT) surface treated with hydrofluoric acid etching, and strontium-loaded nano Ti (NT-Sr) surface treated with SrTiO3 target deposited via magnetron sputtering technique were investigated in parallel for comparison. The results showed that MNT-Sr surfaces were prepared successfully and with high interface bonding strength. Moreover, slow Sr release could be detected when the MNT-Sr and NT-Sr samples were immersed in phosphate-buffered saline. In in vitro experiments, the MNT-Sr surface significantly improved the proliferation and differentiation of osteoblasts compared with the other three groups. Twelve weeks after the four different surface implants were inserted into the distal femurs of 40 rats, the bone–implant contact in the ST, MT, NT-Sr, and MNT-Sr groups were 39.70%±6.00%, 57.60%±7.79%, 46.10%±5.51%, and 70.38%±8.61%, respectively. In terms of the mineral apposition ratio, the MNT-Sr group increased by 129%, 58%, and 25% compared with the values of the ST, MT, and NT-Sr groups, respectively. Moreover, the maximal pullout force in the MNT-Sr group was 1.12-, 0.31-, and 0.69-fold higher than the values of the ST, MT, and NT-Sr groups, respectively. These results suggested that the MNT-Sr surface has a synergistic effect of hierarchical micro/nano-topography and strontium for enhanced osseointegration, and it may be a promising option for clinical use. Compared with the MT surface, the NT-Sr surface significantly improved the differentiation of osteoblasts in vitro. In the in vivo animal experiment, the MT surface significantly enhanced the bone-implant contact and maximal pullout force than the NT-Sr surface. Keywords: surface modification, micro/nanorough, implantLi YQi YGao QNiu QShen MFu QHu KKong LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 4549-4563 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li Y Qi Y Gao Q Niu Q Shen M Fu Q Hu K Kong L Effects of a micro/nano rough strontium-loaded surface on osseointegration
description	Yongfeng Li,1,* Yaping Qi,2,* Qi Gao,3,* Qiang Niu,1 Mingming Shen,2 Qian Fu,1 Kaijin Hu,1 Liang Kong1 1State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi’an, 2Department of Oral and Maxillofacial Surgery, School of Stomatology, the Second Hospital of Hebei Medical University, Shijiazhuang, 3Department of Stomatology, PLA 458 Hospital, Guangzhou, People’s Republic of China *These authors contributed equally to this work Abstract: We developed a hierarchical hybrid micro/nanorough strontium-loaded Ti (MNT-Sr) surface fabricated through hydrofluoric acid etching followed by magnetron sputtering and evaluated the effects of this surface on osseointegration. Samples with a smooth Ti (ST) surface, micro Ti (MT) surface treated with hydrofluoric acid etching, and strontium-loaded nano Ti (NT-Sr) surface treated with SrTiO3 target deposited via magnetron sputtering technique were investigated in parallel for comparison. The results showed that MNT-Sr surfaces were prepared successfully and with high interface bonding strength. Moreover, slow Sr release could be detected when the MNT-Sr and NT-Sr samples were immersed in phosphate-buffered saline. In in vitro experiments, the MNT-Sr surface significantly improved the proliferation and differentiation of osteoblasts compared with the other three groups. Twelve weeks after the four different surface implants were inserted into the distal femurs of 40 rats, the bone–implant contact in the ST, MT, NT-Sr, and MNT-Sr groups were 39.70%±6.00%, 57.60%±7.79%, 46.10%±5.51%, and 70.38%±8.61%, respectively. In terms of the mineral apposition ratio, the MNT-Sr group increased by 129%, 58%, and 25% compared with the values of the ST, MT, and NT-Sr groups, respectively. Moreover, the maximal pullout force in the MNT-Sr group was 1.12-, 0.31-, and 0.69-fold higher than the values of the ST, MT, and NT-Sr groups, respectively. These results suggested that the MNT-Sr surface has a synergistic effect of hierarchical micro/nano-topography and strontium for enhanced osseointegration, and it may be a promising option for clinical use. Compared with the MT surface, the NT-Sr surface significantly improved the differentiation of osteoblasts in vitro. In the in vivo animal experiment, the MT surface significantly enhanced the bone-implant contact and maximal pullout force than the NT-Sr surface. Keywords: surface modification, micro/nanorough, implant
format	article
author	Li Y Qi Y Gao Q Niu Q Shen M Fu Q Hu K Kong L
author_facet	Li Y Qi Y Gao Q Niu Q Shen M Fu Q Hu K Kong L
author_sort	Li Y
title	Effects of a micro/nano rough strontium-loaded surface on osseointegration
title_short	Effects of a micro/nano rough strontium-loaded surface on osseointegration
title_full	Effects of a micro/nano rough strontium-loaded surface on osseointegration
title_fullStr	Effects of a micro/nano rough strontium-loaded surface on osseointegration
title_full_unstemmed	Effects of a micro/nano rough strontium-loaded surface on osseointegration
title_sort	effects of a micro/nano rough strontium-loaded surface on osseointegration
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/4e0c9147c7ec49af8971f5eef1846038
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Effects of a micro/nano rough strontium-loaded surface on osseointegration

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