BASP1 labels neural stem cells in the neurogenic niches of mammalian brain

Abstract The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistoche...

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Autores principales:	Louis N. Manganas, Irene Durá, Sivan Osenberg, Faith Semerci, Mehmet Tosun, Rachana Mishra, Luke Parkitny, Juan M. Encinas, Mirjana Maletic-Savatic
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e13a8dbe31b446bb00d9ddc980acb5a
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spelling	oai:doaj.org-article:4e13a8dbe31b446bb00d9ddc980acb5a2021-12-02T11:35:40ZBASP1 labels neural stem cells in the neurogenic niches of mammalian brain10.1038/s41598-021-85129-12045-2322https://doaj.org/article/4e13a8dbe31b446bb00d9ddc980acb5a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85129-1https://doaj.org/toc/2045-2322Abstract The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistochemical data showed that the NSC-6 antibody recognized NSCs in the developing and postnatal murine brains as well as in human brain organoids. Mass spectrometry revealed the identity of the NSC-6 epitope as brain abundant, membrane-attached signal protein 1 (BASP1), a signaling protein that plays a key role in neurite outgrowth and plasticity. Western blot analysis using the NSC-6 antibody demonstrated multiple BASP1 isoforms with varying degrees of expression and correlating with distinct developmental stages. Herein, we describe the expression of BASP1 in NSCs in the developing and postnatal mammalian brains and human brain organoids, and demonstrate that the NSC-6 antibody may be a useful marker of these cells.Louis N. ManganasIrene DuráSivan OsenbergFaith SemerciMehmet TosunRachana MishraLuke ParkitnyJuan M. EncinasMirjana Maletic-SavaticNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Louis N. Manganas Irene Durá Sivan Osenberg Faith Semerci Mehmet Tosun Rachana Mishra Luke Parkitny Juan M. Encinas Mirjana Maletic-Savatic BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
description	Abstract The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistochemical data showed that the NSC-6 antibody recognized NSCs in the developing and postnatal murine brains as well as in human brain organoids. Mass spectrometry revealed the identity of the NSC-6 epitope as brain abundant, membrane-attached signal protein 1 (BASP1), a signaling protein that plays a key role in neurite outgrowth and plasticity. Western blot analysis using the NSC-6 antibody demonstrated multiple BASP1 isoforms with varying degrees of expression and correlating with distinct developmental stages. Herein, we describe the expression of BASP1 in NSCs in the developing and postnatal mammalian brains and human brain organoids, and demonstrate that the NSC-6 antibody may be a useful marker of these cells.
format	article
author	Louis N. Manganas Irene Durá Sivan Osenberg Faith Semerci Mehmet Tosun Rachana Mishra Luke Parkitny Juan M. Encinas Mirjana Maletic-Savatic
author_facet	Louis N. Manganas Irene Durá Sivan Osenberg Faith Semerci Mehmet Tosun Rachana Mishra Luke Parkitny Juan M. Encinas Mirjana Maletic-Savatic
author_sort	Louis N. Manganas
title	BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
title_short	BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
title_full	BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
title_fullStr	BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
title_full_unstemmed	BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
title_sort	basp1 labels neural stem cells in the neurogenic niches of mammalian brain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4e13a8dbe31b446bb00d9ddc980acb5a
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BASP1 labels neural stem cells in the neurogenic niches of mammalian brain

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