Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico

ABSTRACT High-throughput cultivation studies have been successful at bringing numerous important marine bacterioplankton lineages into culture, yet these frequently utilize natural seawater media that can hamper portability, reproducibility, and downstream characterization efforts. Here we report th...

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Autores principales:	Michael W. Henson, David M. Pitre, Jessica Lee Weckhorst, V. Celeste Lanclos, Austen T. Webber, J. Cameron Thrash
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Gulf of Mexico SAR11 artificial seawater coastal microbiology high-throughput culturing marine microbiology Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4e145527e60047c3bdb3b191f5a8ed73
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spelling	oai:doaj.org-article:4e145527e60047c3bdb3b191f5a8ed732021-11-15T15:21:22ZArtificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico10.1128/mSphere.00028-162379-5042https://doaj.org/article/4e145527e60047c3bdb3b191f5a8ed732016-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00028-16https://doaj.org/toc/2379-5042ABSTRACT High-throughput cultivation studies have been successful at bringing numerous important marine bacterioplankton lineages into culture, yet these frequently utilize natural seawater media that can hamper portability, reproducibility, and downstream characterization efforts. Here we report the results of seven experiments with a set of newly developed artificial seawater media and evaluation of cultivation success via comparison with community sequencing data from the inocula. Eighty-two new isolates represent highly important marine clades, including SAR116, OM60/NOR5, SAR92, Roseobacter, and SAR11. For many, isolation with an artificial seawater medium is unprecedented, and several organisms are also the first of their type from the Gulf of Mexico. Community analysis revealed that many isolates were among the 20 most abundant organisms in their source inoculum. This method will expand the accessibility of bacterioplankton cultivation experiments and improve repeatability by avoiding normal compositional changes in natural seawater. IMPORTANCE The difficulty in cultivating many microbial taxa vexes researchers intent on understanding the contributions of these organisms to natural systems, particularly when these organisms are numerically abundant, and many cultivation attempts recover only rare taxa. Efforts to improve this conundrum with marine bacterioplankton have been successful with natural seawater media, but that approach suffers from a number of drawbacks and there have been no comparable artificial alternatives created in the laboratory. This work demonstrates that a newly developed suite of artificial-seawater media can successfully cultivate many of the most abundant taxa from seawater samples and many taxa previously only cultivated with natural-seawater media. This methodology therefore significantly simplifies efforts to cultivate bacterioplankton and greatly improves our ability to perform physiological characterization of cultures postisolation.Michael W. HensonDavid M. PitreJessica Lee WeckhorstV. Celeste LanclosAusten T. WebberJ. Cameron ThrashAmerican Society for MicrobiologyarticleGulf of MexicoSAR11artificial seawatercoastal microbiologyhigh-throughput culturingmarine microbiologyMicrobiologyQR1-502ENmSphere, Vol 1, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gulf of Mexico SAR11 artificial seawater coastal microbiology high-throughput culturing marine microbiology Microbiology QR1-502
spellingShingle	Gulf of Mexico SAR11 artificial seawater coastal microbiology high-throughput culturing marine microbiology Microbiology QR1-502 Michael W. Henson David M. Pitre Jessica Lee Weckhorst V. Celeste Lanclos Austen T. Webber J. Cameron Thrash Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico
description	ABSTRACT High-throughput cultivation studies have been successful at bringing numerous important marine bacterioplankton lineages into culture, yet these frequently utilize natural seawater media that can hamper portability, reproducibility, and downstream characterization efforts. Here we report the results of seven experiments with a set of newly developed artificial seawater media and evaluation of cultivation success via comparison with community sequencing data from the inocula. Eighty-two new isolates represent highly important marine clades, including SAR116, OM60/NOR5, SAR92, Roseobacter, and SAR11. For many, isolation with an artificial seawater medium is unprecedented, and several organisms are also the first of their type from the Gulf of Mexico. Community analysis revealed that many isolates were among the 20 most abundant organisms in their source inoculum. This method will expand the accessibility of bacterioplankton cultivation experiments and improve repeatability by avoiding normal compositional changes in natural seawater. IMPORTANCE The difficulty in cultivating many microbial taxa vexes researchers intent on understanding the contributions of these organisms to natural systems, particularly when these organisms are numerically abundant, and many cultivation attempts recover only rare taxa. Efforts to improve this conundrum with marine bacterioplankton have been successful with natural seawater media, but that approach suffers from a number of drawbacks and there have been no comparable artificial alternatives created in the laboratory. This work demonstrates that a newly developed suite of artificial-seawater media can successfully cultivate many of the most abundant taxa from seawater samples and many taxa previously only cultivated with natural-seawater media. This methodology therefore significantly simplifies efforts to cultivate bacterioplankton and greatly improves our ability to perform physiological characterization of cultures postisolation.
format	article
author	Michael W. Henson David M. Pitre Jessica Lee Weckhorst V. Celeste Lanclos Austen T. Webber J. Cameron Thrash
author_facet	Michael W. Henson David M. Pitre Jessica Lee Weckhorst V. Celeste Lanclos Austen T. Webber J. Cameron Thrash
author_sort	Michael W. Henson
title	Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico
title_short	Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico
title_full	Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico
title_fullStr	Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico
title_full_unstemmed	Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico
title_sort	artificial seawater media facilitate cultivating members of the microbial majority from the gulf of mexico
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/4e145527e60047c3bdb3b191f5a8ed73
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Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico

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