Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development

Introduction Aberrant expression of long non-coding RNAs (lncRNAs) has been implicated in various diseases, including cancer. However, little is known about lncRNAs in human brain gliomas. Material and methods We examined lncRNA profiles from three glioma specimens using lncRNA expression profiling...

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Autores principales:	Yi Ding, Xinfa Wang, Junchen Pan, Minjun Ji, Zhengxiang Luo, Penglai Zhao, Yansong Zhang, Gang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	microarrays glioma lncrnas Medicine R
Acceso en línea:	https://doaj.org/article/4e1b120e46fd4e48a5bacaca46e59877
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spelling	oai:doaj.org-article:4e1b120e46fd4e48a5bacaca46e598772021-12-02T16:59:25ZAberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development1734-19221896-915110.5114/aoms.2020.91290https://doaj.org/article/4e1b120e46fd4e48a5bacaca46e598772019-12-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Aberrant-expression-of-long-non-coding-RNAs-lncRNAs-is-involved-in-brain-glioma-development,76193,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Aberrant expression of long non-coding RNAs (lncRNAs) has been implicated in various diseases, including cancer. However, little is known about lncRNAs in human brain gliomas. Material and methods We examined lncRNA profiles from three glioma specimens using lncRNA expression profiling microarrays. Quantitative real-time RT-PCR was used to analyze the differential expression of raw intensities of lncRNA expression in glioma and peritumoral tissues. Results We found 4858 lncRNAs to be differentially expressed between tumor tissue and peritumoral tissue. Of these, 2845 lncRNAs were up-regulated (fold change > 3.0) and 2013 were down-regulated (fold change 3.0) and 1804 that were down-regulated (fold change < 1/3). Consistent with the microarray data, qPCR confirmed differential expression of these 6 lncRNAs (ak125809, ak098473, uc002ehu.1, bc043564, NR_027322, and uc003qmb.2) between tumor and peritumoral tissue. We next established co-expression networks of differentially expressed lncRNAs and mRNAs. Many mRNAs, such as LOC729991, NUDCD1, SHC3, PDGFA, and MDM2, and lncRNAs, such as ENST00000425922, ENST00000455568, uc002ukz.1, ENST00000502715, and NR_027873, have been shown to play important roles in glioma development. Consistent with this, pathway analysis revealed that “GLIOMA” (KEGG Pathway ID: hsa05214) was significantly enriched in tumor tissue. Conclusions Our data suggest that altered expression of lncRNAs may be a critical determinant of tumorigenesis in glioma patients.Yi DingXinfa WangJunchen PanMinjun JiZhengxiang LuoPenglai ZhaoYansong ZhangGang WangTermedia Publishing HousearticlemicroarraysgliomalncrnasMedicineRENArchives of Medical Science, Vol 16, Iss 1, Pp 177-188 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	microarrays glioma lncrnas Medicine R
spellingShingle	microarrays glioma lncrnas Medicine R Yi Ding Xinfa Wang Junchen Pan Minjun Ji Zhengxiang Luo Penglai Zhao Yansong Zhang Gang Wang Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development
description	Introduction Aberrant expression of long non-coding RNAs (lncRNAs) has been implicated in various diseases, including cancer. However, little is known about lncRNAs in human brain gliomas. Material and methods We examined lncRNA profiles from three glioma specimens using lncRNA expression profiling microarrays. Quantitative real-time RT-PCR was used to analyze the differential expression of raw intensities of lncRNA expression in glioma and peritumoral tissues. Results We found 4858 lncRNAs to be differentially expressed between tumor tissue and peritumoral tissue. Of these, 2845 lncRNAs were up-regulated (fold change > 3.0) and 2013 were down-regulated (fold change 3.0) and 1804 that were down-regulated (fold change < 1/3). Consistent with the microarray data, qPCR confirmed differential expression of these 6 lncRNAs (ak125809, ak098473, uc002ehu.1, bc043564, NR_027322, and uc003qmb.2) between tumor and peritumoral tissue. We next established co-expression networks of differentially expressed lncRNAs and mRNAs. Many mRNAs, such as LOC729991, NUDCD1, SHC3, PDGFA, and MDM2, and lncRNAs, such as ENST00000425922, ENST00000455568, uc002ukz.1, ENST00000502715, and NR_027873, have been shown to play important roles in glioma development. Consistent with this, pathway analysis revealed that “GLIOMA” (KEGG Pathway ID: hsa05214) was significantly enriched in tumor tissue. Conclusions Our data suggest that altered expression of lncRNAs may be a critical determinant of tumorigenesis in glioma patients.
format	article
author	Yi Ding Xinfa Wang Junchen Pan Minjun Ji Zhengxiang Luo Penglai Zhao Yansong Zhang Gang Wang
author_facet	Yi Ding Xinfa Wang Junchen Pan Minjun Ji Zhengxiang Luo Penglai Zhao Yansong Zhang Gang Wang
author_sort	Yi Ding
title	Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development
title_short	Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development
title_full	Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development
title_fullStr	Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development
title_full_unstemmed	Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development
title_sort	aberrant expression of long non-coding rnas (lncrnas) is involved in brain glioma development
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/4e1b120e46fd4e48a5bacaca46e59877
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Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development

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