ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer

Ovarian cancer has the highest mortality rate among gynecological tumors. This is based on late diagnosis and the lack of early symptoms. To improve early detection, it is essential to find reliable biomarkers. The metalloprotease ADAM17 could be a potential marker, as it is highly expressed in many...

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Autores principales:	Christoph Rogmans, Jan Dominik Kuhlmann, Gerrit Hugendieck, Theresa Link, Norbert Arnold, Jörg Paul Weimer, Inken Flörkemeier, Anna-Christina Rambow, Wolfgang Lieb, Nicolai Maass, Dirk O. Bauerschlag, Nina Hedemann
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ovarian cancer ADAM17 serum ascites tumor marker early detection Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/4e1ccf5ac3f349e3bb09092653ec41ae
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spelling	oai:doaj.org-article:4e1ccf5ac3f349e3bb09092653ec41ae2021-11-11T15:35:13ZADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer10.3390/cancers132155632072-6694https://doaj.org/article/4e1ccf5ac3f349e3bb09092653ec41ae2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5563https://doaj.org/toc/2072-6694Ovarian cancer has the highest mortality rate among gynecological tumors. This is based on late diagnosis and the lack of early symptoms. To improve early detection, it is essential to find reliable biomarkers. The metalloprotease ADAM17 could be a potential marker, as it is highly expressed in many solid tumors, including ovarian and breast cancer. The aim of this work is to evaluate the relevance of ADAM17 as a potential diagnostic blood-based biomarker in ovarian cancer. Ovarian cancer cell lines IGROV-1 and A2780, as well as primary patient-derived tumor cells obtained from tumor tissue and ascitic fluid, were cultured to analyze ADAM17 abundance in the culture supernatant. In a translational approach, a cohort of 117 well-characterized ovarian cancer patients was assembled and ADAM17 levels in serum and corresponding ascitic fluid were determined at primary diagnosis. ADAM17 was quantified by enzyme-linked immunosorbent assay (ELISA). In the present study, ADAM17 was detected in the culture supernatant of ovarian cancer cell lines and primary cells. In addition, ADAM17 was found in serum and ascites of ovarian cancer patients. ADAM17 level was significantly increased in ovarian cancer patients compared to an age-matched control group (<i>p</i> < 0.0001). Importantly early FIGO I/II stages, which would not have been detected by CA-125, were associated with higher ADAM17 concentrations (<i>p</i> = 0.007). This is the first study proposing ADAM17 as a serum tumor marker in the setting of a gynecological tumor disease. Usage of ADAM17 in combination with CA-125 and other markers could help detect early stages of ovarian cancer.Christoph RogmansJan Dominik KuhlmannGerrit HugendieckTheresa LinkNorbert ArnoldJörg Paul WeimerInken FlörkemeierAnna-Christina RambowWolfgang LiebNicolai MaassDirk O. BauerschlagNina HedemannMDPI AGarticleovarian cancerADAM17serumascitestumor markerearly detectionNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5563, p 5563 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ovarian cancer ADAM17 serum ascites tumor marker early detection Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	ovarian cancer ADAM17 serum ascites tumor marker early detection Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Christoph Rogmans Jan Dominik Kuhlmann Gerrit Hugendieck Theresa Link Norbert Arnold Jörg Paul Weimer Inken Flörkemeier Anna-Christina Rambow Wolfgang Lieb Nicolai Maass Dirk O. Bauerschlag Nina Hedemann ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer
description	Ovarian cancer has the highest mortality rate among gynecological tumors. This is based on late diagnosis and the lack of early symptoms. To improve early detection, it is essential to find reliable biomarkers. The metalloprotease ADAM17 could be a potential marker, as it is highly expressed in many solid tumors, including ovarian and breast cancer. The aim of this work is to evaluate the relevance of ADAM17 as a potential diagnostic blood-based biomarker in ovarian cancer. Ovarian cancer cell lines IGROV-1 and A2780, as well as primary patient-derived tumor cells obtained from tumor tissue and ascitic fluid, were cultured to analyze ADAM17 abundance in the culture supernatant. In a translational approach, a cohort of 117 well-characterized ovarian cancer patients was assembled and ADAM17 levels in serum and corresponding ascitic fluid were determined at primary diagnosis. ADAM17 was quantified by enzyme-linked immunosorbent assay (ELISA). In the present study, ADAM17 was detected in the culture supernatant of ovarian cancer cell lines and primary cells. In addition, ADAM17 was found in serum and ascites of ovarian cancer patients. ADAM17 level was significantly increased in ovarian cancer patients compared to an age-matched control group (<i>p</i> < 0.0001). Importantly early FIGO I/II stages, which would not have been detected by CA-125, were associated with higher ADAM17 concentrations (<i>p</i> = 0.007). This is the first study proposing ADAM17 as a serum tumor marker in the setting of a gynecological tumor disease. Usage of ADAM17 in combination with CA-125 and other markers could help detect early stages of ovarian cancer.
format	article
author	Christoph Rogmans Jan Dominik Kuhlmann Gerrit Hugendieck Theresa Link Norbert Arnold Jörg Paul Weimer Inken Flörkemeier Anna-Christina Rambow Wolfgang Lieb Nicolai Maass Dirk O. Bauerschlag Nina Hedemann
author_facet	Christoph Rogmans Jan Dominik Kuhlmann Gerrit Hugendieck Theresa Link Norbert Arnold Jörg Paul Weimer Inken Flörkemeier Anna-Christina Rambow Wolfgang Lieb Nicolai Maass Dirk O. Bauerschlag Nina Hedemann
author_sort	Christoph Rogmans
title	ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer
title_short	ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer
title_full	ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer
title_fullStr	ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer
title_full_unstemmed	ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer
title_sort	adam17—a potential blood-based biomarker for detection of early-stage ovarian cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4e1ccf5ac3f349e3bb09092653ec41ae
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ADAM17—A Potential Blood-Based Biomarker for Detection of Early-Stage Ovarian Cancer

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