The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid

Salicylic acid (SA) and jasmonic acid (JA) are essential plant immune hormones, which could induce plant resistance to multiple pathogens. However, whether common components are employed by both SA and JA to induce defense is largely unknown. In this study, we found that the <i>enhanced diseas...

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Autores principales:	Nengxu Sun, Xiangjiu Kong, Yueyan Liu, Tingting Gong, Xiaoyong Gu, Lijing Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	salicylic acid jasmonic acid THO/TREX complex alternative splicing induced defense Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4e1f8113d74f4ab6abd31c06b760b531
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spelling	oai:doaj.org-article:4e1f8113d74f4ab6abd31c06b760b5312021-11-25T17:54:14ZThe THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid10.3390/ijms2222121971422-00671661-6596https://doaj.org/article/4e1f8113d74f4ab6abd31c06b760b5312021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12197https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Salicylic acid (SA) and jasmonic acid (JA) are essential plant immune hormones, which could induce plant resistance to multiple pathogens. However, whether common components are employed by both SA and JA to induce defense is largely unknown. In this study, we found that the <i>enhanced disease susceptibility 8</i> (<i>EDS8</i>) mutant was compromised in plant defenses to hemibiotrophic pathogen <i>Pseudomonas syringae pv. maculicola</i> ES4326 and necrotrophic pathogen <i>Botrytis cinerea</i>, and was deficient in plant responses to both SA and JA. The <i>EDS8</i> was identified to be <i>THO1</i>, which encodes a subunit of the THO/TREX complex, by using mapping-by-sequencing. To check whether the EDS8 itself or the THO/TREX complex mediates SA and JA signaling, the mutant of another subunit of the THO/TREX complex, THO3, was tested. <i>THO3</i> mutation reduced both SA and JA induced defenses, indicating that the THO/TREX complex is critical for plant responses to these two hormones. We further proved that the THO/TREX interacting protein SERRATE, a factor regulating alternative splicing (AS), was involved in plant responses to SA and JA. Thus, the AS events in the <i>eds</i>8 mutant after SA or JA treatment were determined, and we found that the SA and JA induced different alternative splicing events were majorly modulated by EDS8. In summary, our study proves that the THO/TREX complex active in AS is involved in both SA and JA induced plant defenses.Nengxu SunXiangjiu KongYueyan LiuTingting GongXiaoyong GuLijing LiuMDPI AGarticlesalicylic acidjasmonic acidTHO/TREX complexalternative splicinginduced defenseBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12197, p 12197 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	salicylic acid jasmonic acid THO/TREX complex alternative splicing induced defense Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	salicylic acid jasmonic acid THO/TREX complex alternative splicing induced defense Biology (General) QH301-705.5 Chemistry QD1-999 Nengxu Sun Xiangjiu Kong Yueyan Liu Tingting Gong Xiaoyong Gu Lijing Liu The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid
description	Salicylic acid (SA) and jasmonic acid (JA) are essential plant immune hormones, which could induce plant resistance to multiple pathogens. However, whether common components are employed by both SA and JA to induce defense is largely unknown. In this study, we found that the <i>enhanced disease susceptibility 8</i> (<i>EDS8</i>) mutant was compromised in plant defenses to hemibiotrophic pathogen <i>Pseudomonas syringae pv. maculicola</i> ES4326 and necrotrophic pathogen <i>Botrytis cinerea</i>, and was deficient in plant responses to both SA and JA. The <i>EDS8</i> was identified to be <i>THO1</i>, which encodes a subunit of the THO/TREX complex, by using mapping-by-sequencing. To check whether the EDS8 itself or the THO/TREX complex mediates SA and JA signaling, the mutant of another subunit of the THO/TREX complex, THO3, was tested. <i>THO3</i> mutation reduced both SA and JA induced defenses, indicating that the THO/TREX complex is critical for plant responses to these two hormones. We further proved that the THO/TREX interacting protein SERRATE, a factor regulating alternative splicing (AS), was involved in plant responses to SA and JA. Thus, the AS events in the <i>eds</i>8 mutant after SA or JA treatment were determined, and we found that the SA and JA induced different alternative splicing events were majorly modulated by EDS8. In summary, our study proves that the THO/TREX complex active in AS is involved in both SA and JA induced plant defenses.
format	article
author	Nengxu Sun Xiangjiu Kong Yueyan Liu Tingting Gong Xiaoyong Gu Lijing Liu
author_facet	Nengxu Sun Xiangjiu Kong Yueyan Liu Tingting Gong Xiaoyong Gu Lijing Liu
author_sort	Nengxu Sun
title	The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid
title_short	The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid
title_full	The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid
title_fullStr	The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid
title_full_unstemmed	The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid
title_sort	tho/trex complex active in alternative splicing mediates plant responses to salicylic acid and jasmonic acid
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4e1f8113d74f4ab6abd31c06b760b531
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The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid

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