Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette

Abstract Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effe...

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Autores principales:	Yuanyuan Huang, Lu Li, Shan Xie, Nannan Zhao, Shuangyan Han, Ying Lin, Suiping Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e203a7658e84e73b75e6f0c252d6460
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spelling	oai:doaj.org-article:4e203a7658e84e73b75e6f0c252d64602021-12-02T16:08:22ZRecombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette10.1038/s41598-017-08352-92045-2322https://doaj.org/article/4e203a7658e84e73b75e6f0c252d64602017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08352-9https://doaj.org/toc/2045-2322Abstract Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effective and sequential deletion method for C. glutamicum ATCC14067 using the exonuclease-recombinase pair RecE + RecT (RecET) for recombineering via a designed self-excisable linear double-strand DNA (dsDNA) cassette, which contains the Cre/loxP system, to accomplish markerless deletion. To the best of our knowledge, this is the first effective and simple strategy for recombination with markerless deletion in C. glutamicum ATCC14067. This strategy provides a simple markerless deletion strategy for C. glutamicum and builds a solid basis for producer construction.Yuanyuan HuangLu LiShan XieNannan ZhaoShuangyan HanYing LinSuiping ZhengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuanyuan Huang Lu Li Shan Xie Nannan Zhao Shuangyan Han Ying Lin Suiping Zheng Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
description	Abstract Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effective and sequential deletion method for C. glutamicum ATCC14067 using the exonuclease-recombinase pair RecE + RecT (RecET) for recombineering via a designed self-excisable linear double-strand DNA (dsDNA) cassette, which contains the Cre/loxP system, to accomplish markerless deletion. To the best of our knowledge, this is the first effective and simple strategy for recombination with markerless deletion in C. glutamicum ATCC14067. This strategy provides a simple markerless deletion strategy for C. glutamicum and builds a solid basis for producer construction.
format	article
author	Yuanyuan Huang Lu Li Shan Xie Nannan Zhao Shuangyan Han Ying Lin Suiping Zheng
author_facet	Yuanyuan Huang Lu Li Shan Xie Nannan Zhao Shuangyan Han Ying Lin Suiping Zheng
author_sort	Yuanyuan Huang
title	Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
title_short	Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
title_full	Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
title_fullStr	Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
title_full_unstemmed	Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
title_sort	recombineering using recet in corynebacterium glutamicum atcc14067 via a self-excisable cassette
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4e203a7658e84e73b75e6f0c252d6460
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Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette

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