Characterization of Enterococcus faecalis in different culture conditions

Abstract The aim of this study was to investigate how carbohydrates (glucose or sucrose) affect the characteristics of Enterococcus faecalis (E. faecalis) planktonic and biofilm in vitro. For this study, E. faecalis was cultured in tryptone-yeast extract broth with 0% glucose + 0% sucrose, 0.5% gluc...

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Autores principales:	Mi-Ah Kim, Vinicius Rosa, Kyung-San Min
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e20dcdad49c4d1aacec848de89159af
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spelling	oai:doaj.org-article:4e20dcdad49c4d1aacec848de89159af2021-12-02T12:03:15ZCharacterization of Enterococcus faecalis in different culture conditions10.1038/s41598-020-78998-52045-2322https://doaj.org/article/4e20dcdad49c4d1aacec848de89159af2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78998-5https://doaj.org/toc/2045-2322Abstract The aim of this study was to investigate how carbohydrates (glucose or sucrose) affect the characteristics of Enterococcus faecalis (E. faecalis) planktonic and biofilm in vitro. For this study, E. faecalis was cultured in tryptone-yeast extract broth with 0% glucose + 0% sucrose, 0.5% glucose, 1% glucose, 0.5% sucrose, or 1% sucrose. Viability of E. faecalis was examined by colony forming unit counting assays. Biofilm formation was assessed by measuring extracellular DNA (eDNA), a component of the biofilm matrix. Quantitative real-time PCR (qRT-PCR) was performed to investigate the expression of virulence-associated genes. Field emission scanning electron microscopy analysis, confocal laser scanning microscopy analysis, and crystal violet colorimetric assay were conducted to study E. faecalis biofilms. E. faecalis showed the highest viability and eDNA levels in 1% sucrose medium in biofilms. The result of qRT-PCR showed that the virulence-associated genes expressed highest in 1% sucrose-grown biofilms and in 1% glucose-grown planktonic cultures. E. faecalis showed highly aggregated biofilms and higher bacteria and exopolysaccharide (EPS) bio-volume in sucrose than in 0% glucose + 0% sucrose or glucose. The results indicate that the production of eDNA and EPS and expression of virulence-associated genes in E. faecalis are affected by the concentration of carbohydrates in biofilm or planktonic culture.Mi-Ah KimVinicius RosaKyung-San MinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mi-Ah Kim Vinicius Rosa Kyung-San Min Characterization of Enterococcus faecalis in different culture conditions
description	Abstract The aim of this study was to investigate how carbohydrates (glucose or sucrose) affect the characteristics of Enterococcus faecalis (E. faecalis) planktonic and biofilm in vitro. For this study, E. faecalis was cultured in tryptone-yeast extract broth with 0% glucose + 0% sucrose, 0.5% glucose, 1% glucose, 0.5% sucrose, or 1% sucrose. Viability of E. faecalis was examined by colony forming unit counting assays. Biofilm formation was assessed by measuring extracellular DNA (eDNA), a component of the biofilm matrix. Quantitative real-time PCR (qRT-PCR) was performed to investigate the expression of virulence-associated genes. Field emission scanning electron microscopy analysis, confocal laser scanning microscopy analysis, and crystal violet colorimetric assay were conducted to study E. faecalis biofilms. E. faecalis showed the highest viability and eDNA levels in 1% sucrose medium in biofilms. The result of qRT-PCR showed that the virulence-associated genes expressed highest in 1% sucrose-grown biofilms and in 1% glucose-grown planktonic cultures. E. faecalis showed highly aggregated biofilms and higher bacteria and exopolysaccharide (EPS) bio-volume in sucrose than in 0% glucose + 0% sucrose or glucose. The results indicate that the production of eDNA and EPS and expression of virulence-associated genes in E. faecalis are affected by the concentration of carbohydrates in biofilm or planktonic culture.
format	article
author	Mi-Ah Kim Vinicius Rosa Kyung-San Min
author_facet	Mi-Ah Kim Vinicius Rosa Kyung-San Min
author_sort	Mi-Ah Kim
title	Characterization of Enterococcus faecalis in different culture conditions
title_short	Characterization of Enterococcus faecalis in different culture conditions
title_full	Characterization of Enterococcus faecalis in different culture conditions
title_fullStr	Characterization of Enterococcus faecalis in different culture conditions
title_full_unstemmed	Characterization of Enterococcus faecalis in different culture conditions
title_sort	characterization of enterococcus faecalis in different culture conditions
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4e20dcdad49c4d1aacec848de89159af
work_keys_str_mv	AT miahkim characterizationofenterococcusfaecalisindifferentcultureconditions AT viniciusrosa characterizationofenterococcusfaecalisindifferentcultureconditions AT kyungsanmin characterizationofenterococcusfaecalisindifferentcultureconditions
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Characterization of Enterococcus faecalis in different culture conditions

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